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Proteins and peptidesOur latest ELISA kit: Human Tau (phospho T217) - Intracellular
Highly sensitive kit offering the most promising biomarkers for Alzheimer’s disease diagnostics. Learn about all product ranges with our product overviews.
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Recombinant Human Tau (mutated N352H) protein is a Human Full Length protein, in the 1 to 383 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Alternative names=Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPTL, MTBT1, MAPT, TAU
- Purity
>85% Densitometry
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
- Sequence
M A E P R Q E F E V M E D H A G T Y G L G D R K D Q G G Y T M H Q D Q E G D T D A G L K A E E A G I G D T P S L E D E A A G H V T Q A R M V S K S K D G T G S D D K K A K G A D G K T K I A T P R G A A P P G Q K G Q A N A T R I P A K T P P A P K T P P S S G E P P K S G D R S G Y S S P G S P G T P G S R S R T P S L P T P P T R E P K K V A V V R T P P K S P S S A K S R L Q T A P V P M P D L K N V K S K I G S T E N L K H Q P G G G K V Q I I N K K L D L S N V Q S K C G S K D N I K H V P G G G S V Q I V Y K P V D L S K V T S K C G S L G N I H H K P G G G Q V E V K S E K L D F K D R V Q S K I G S L D N I T H V P G G G N K K I E T H K L T F R E N A K A K T D H G A E I V Y K S P V V S G D T S P R H L S H V S S T G S I D M V D S P Q L A T L A D E V S A S L A K Q G L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Images
Target data
Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
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Recombinant Human Tau (mutated N352H) protein is a Human Full Length protein, in the 1 to 383 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Alternative names=Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPTL, MTBT1, MAPT, TAU
Key facts
- Purity
>85% Densitometry
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
- Predicted molecular weight
51 kDa
- Amino acids
1 to 383
- Sequence
M A E P R Q E F E V M E D H A G T Y G L G D R K D Q G G Y T M H Q D Q E G D T D A G L K A E E A G I G D T P S L E D E A A G H V T Q A R M V S K S K D G T G S D D K K A K G A D G K T K I A T P R G A A P P G Q K G Q A N A T R I P A K T P P A P K T P P S S G E P P K S G D R S G Y S S P G S P G T P G S R S R T P S L P T P P T R E P K K V A V V R T P P K S P S S A K S R L Q T A P V P M P D L K N V K S K I G S T E N L K H Q P G G G K V Q I I N K K L D L S N V Q S K C G S K D N I K H V P G G G S V Q I V Y K P V D L S K V T S K C G S L G N I H H K P G G G Q V E V K S E K L D F K D R V Q S K I G S L D N I T H V P G G G N K K I E T H K L T F R E N A K A K T D H G A E I V Y K S P V V S G D T S P R H L S H V S S T G S I D M V D S P Q L A T L A D E V S A S L A K Q G L
- Accession
P10636
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Specifications
- Form
Liquid
General info
- Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
- Post-translational modifications
Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK1, CDK1, CDK5, GSK3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in the form associated with paired helical filaments (PHF-tau)), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1, MARK2, MARK3 or MARK4), causing detachment from microtubules, and their disassembly (PubMed:7706316, PubMed:23666762). Phosphorylation decreases with age. Phosphorylation within tau/MAP's repeat domain or in flanking regions seems to reduce tau/MAP's interaction with, respectively, microtubules or plasma membrane components (PubMed:7706316). Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis. Phosphorylation at Ser-548 by GSK3B reduces ability to bind and stabilize microtubules. Phosphorylation at Ser-579 by BRSK1 and BRSK2 in neurons affects ability to bind microtubules and plays a role in neuron polarization. Phosphorylated at Ser-554, Ser-579, Ser-602, Ser-606 and Ser-669 by PHK. Phosphorylation at Ser-214 by SGK1 mediates microtubule depolymerization and neurite formation in hippocampal neurons. There is a reciprocal down-regulation of phosphorylation and O-GlcNAcylation. Phosphorylation on Ser-717 completely abolishes the O-GlcNAcylation on this site, while phosphorylation on Ser-713 and Ser-721 reduces glycosylation by a factor of 2 and 4 respectively. Phosphorylation on Ser-721 is reduced by about 41.5% by GlcNAcylation on Ser-717. Dephosphorylated at several serine and threonine residues by the serine/threonine phosphatase PPP5C.
- Subcellular localisation
Cytoskeleton
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Storage information
Avoid freeze / thaw cycle
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