JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB246006

Recombinant human Tau (K18) Mutant Monomers (P301L) (Active) (Tag Free)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human Tau (K18) Mutant Monomers (P301L) (Active) (Tag Free) is a Human Fragment protein, in the 241 to 380 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.
2 Images
Functional Studies - Recombinant human Tau (K18) Mutant Monomers (P301L) (Active) (Tag Free) (AB246006)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Tau (K18) Mutant Monomers (P301L) (Active) (Tag Free) (AB246006)

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) in active tau K18/PL monomers (ab246006) over time. Thioflavin T ex = 450 nm, em = 485 nm.

ab246006 (red).

SDS-PAGE - Recombinant human Tau (K18) Mutant Monomers (P301L) (Active) (Tag Free) (AB246006)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Tau (K18) Mutant Monomers (P301L) (Active) (Tag Free) (AB246006)

SDS-PAGE - Recombinant human Tau protein (Active) (ab246006)

Key facts

Purity

>95%

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when active tau PFFs are combined with active tau monomers.

Accession

P10636-8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 0.58% Sodium chloride, 0.24% HEPES

storage-buffer

style
left-column

Complementary Products

Reagents, Controls & Accessories

AB234592

Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)

  • 0
  • 0
Proteins & Peptides

AB269022

Recombinant Human Tau (phospho ) protein (Tag Free)

SDS-PAGE - Recombinant Human Tau (phospho ) protein (Tag Free) (AB269022)

  • 0
  • 0
Proteins & Peptides

AB254309

Recombinant human Alpha-Synuclein protein filament

Sandwich ELISA - Recombinant human Alpha-Synuclein protein filament (AB254309)

  • 0
  • 0
Primary Antibodies

AB281588

Anti-MAP2 antibody [RM1010] - Neuronal Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588)

  • 0
  • 0
Proteins & Peptides

AB114149

Recombinant Human GFAP protein (GST tag N-Terminus)

Western blot - Recombinant Human GFAP protein (GST tag N-Terminus) (AB114149)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"linker":null,"sequence":"MSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVLGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRE","proteinLength":"Fragment","predictedMolecularWeight":"15.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":380,"aminoAcidStart":241,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P10636","tags":[]}]
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
style
left-column

Product protocols

style
left-column

Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information Tau-F P301L

Alternative Names

MAPTL, MTBT1, TAU, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com