Recombinant Human Tau441 protein - BSA and Azide free is a Human Fragment protein, in the 50 to 391 aa range, expressed in Escherichia coli, with >95% purity and suitable for WB.
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Key facts
- Purity
- >95% Densitometry
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- WB
- Biologically active
- No
Amino acid sequence
T P T E D G S E E P G S E T S D A K S T P T A E D V T A P L V D E G A P G K Q A A A Q P H T E I P E G T T A E E A G I G D T P S L E D E A A G H V T Q A R M V S K S K D G T G S D D K K A K G A D G K T K I A T P R G A A P P G Q K G Q A N A T R I P A K T P P A P K T P P S S G E P P K S G D R S G Y S S P G S P G T P G S R S R T P S L P T P P T R E P K K V A V V R T P P K S P S S A K S R L Q T A P V P M P D L K N V K S K I G S T E N L K H Q P G G G K V Q I I N K K L D L S N V Q S K C G S K D N I K H V P G G G S V Q I V Y K P V D L S K V T S K C G S L G N I H H K P G G G Q V E V K S E K L D F K D R V Q S K I G S L D N I T H V P G G G N K K I E T H K L T F R E N A K A K T D H G A E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Recommended products
Recombinant Human Tau441 protein - BSA and Azide free is a Human Fragment protein, in the 50 to 391 aa range, expressed in Escherichia coli, with >95% purity and suitable for WB.
Key facts
- Purity
- >95% Densitometry
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- WB
- Biologically active
- No
- Accession
- P10636-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 0.88% Sodium chloride, 0.79% Tris HCl, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence
- T P T E D G S E E P G S E T S D A K S T P T A E D V T A P L V D E G A P G K Q A A A Q P H T E I P E G T T A E E A G I G D T P S L E D E A A G H V T Q A R M V S K S K D G T G S D D K K A K G A D G K T K I A T P R G A A P P G Q K G Q A N A T R I P A K T P P A P K T P P S S G E P P K S G D R S G Y S S P G S P G T P G S R S R T P S L P T P P T R E P K K V A V V R T P P K S P S S A K S R L Q T A P V P M P D L K N V K S K I G S T E N L K H Q P G G G K V Q I I N K K L D L S N V Q S K C G S K D N I K H V P G G G S V Q I V Y K P V D L S K V T S K C G S L G N I H H K P G G G Q V E V K S E K L D F K D R V Q S K I G S L D N I T H V P G G G N K K I E T H K L T F R E N A K A K T D H G A E
- Accession
- P10636
- Protein length
- Fragment
- Predicted molecular weight
- 35.3 kDa
- Amino acids
- 50 to 391
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
- Post-translational modifications
Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK1, CDK1, CDK5, GSK3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in the form associated with paired helical filaments (PHF-tau)), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1, MARK2, MARK3 or MARK4), causing detachment from microtubules, and their disassembly (PubMed:23666762, PubMed:7706316). Phosphorylation decreases with age. Phosphorylation within tau/MAP's repeat domain or in flanking regions seems to reduce tau/MAP's interaction with, respectively, microtubules or plasma membrane components (PubMed:7706316). Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis. Phosphorylation at Ser-548 by GSK3B reduces ability to bind and stabilize microtubules. Phosphorylation at Ser-579 by BRSK1 and BRSK2 in neurons affects ability to bind microtubules and plays a role in neuron polarization. Phosphorylated at Ser-554, Ser-579, Ser-602, Ser-606 and Ser-669 by PHK. Phosphorylation at Ser-214 by SGK1 mediates microtubule depolymerization and neurite formation in hippocampal neurons. There is a reciprocal down-regulation of phosphorylation and O-GlcNAcylation. Phosphorylation on Ser-717 completely abolishes the O-GlcNAcylation on this site, while phosphorylation on Ser-713 and Ser-721 reduces glycosylation by a factor of 2 and 4 respectively. Phosphorylation on Ser-721 is reduced by about 41.5% by GlcNAcylation on Ser-717. Dephosphorylated at several serine and threonine residues by the serine/threonine phosphatase PPP5C.
- Subcellular localisation
- Cytoskeleton
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tau441 also known as microtubule-associated protein tau (MAPT) plays an important role in stabilizing microtubules within neuronal cells. This protein has a molecular weight of approximately 50 kilodaltons. Tau441 is most highly expressed in neurons of the central nervous system. It assists in maintaining neuronal structure and due to its microtubule-binding domains contributes to proper axonal transport. The proper function of Tau441 is essential for neuronal health and function.
- Biological function summary
Tau441 is an important component in the assembly and stability of microtubule structures. It associates with other tau isoforms to form a microtubule lattice complex important for neuron polarization and intracellular transport. Tau441 influences cell signaling and synaptic function by regulating microtubule dynamics. It also interacts with motor proteins to facilitate the transport of organelles playing a significant role in maintaining neuron structure and functionality under physiological conditions.
- Pathways
Tau441 is involved in the MAP kinase signaling pathway and the regulation of cytoskeleton organization. It interacts with proteins like kinases that phosphorylate tau affecting its microtubule-binding capability highlighting its role in intracellular transport processes. Tau441 modulates the structure and dynamics of microtubules enabling effective transmission of cellular signals and materials throughout the neuron which is vital in maintaining neuronal integrity and function.
- Associated diseases and disorders
Tau441 is notably associated with Alzheimer's disease and frontotemporal dementia. Abnormal phosphorylation of Tau441 results in the formation of neurofibrillary tangles a hallmark of Alzheimer's disease. The dysregulated interaction between Tau441 and kinases like glycogen synthase kinase-3β (GSK-3β) leads to tau hyperphosphorylation contributing to neuronal degeneration. These pathological changes in Tau441 are linked to the onset and progression of neurodegenerative diseases emphasizing the importance of studying and targeting Tau441 in clinical research and therapeutics.
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SDS-PAGE - Recombinant Human Tau441 protein - BSA and Azide free (ab172192)
SDS-PAGE analysis of ab172192
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