Recombinant Human TCF-4 protein is a Human Fragment protein, in the 1 to 507 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M K F K Q C R C S D T G L C C L D H E G K A E V Y A P S A S T A D Y N R D S P G Y P S S K P A T S T F P S S F F M Q D G H H S S D P W S S S S G M N Q P G Y A G M L G N S S H I P Q S S S Y C S L H P H E R L S Y P S H S S A D I N S S L P P M S T F H R S G T N H Y S T S S C T P P A N G T D S I M A N R G S G A A G S S Q T G D A L G K A L A S I Y S P D H T N N S F S S N P S T P V G S P P S L S A G T A V W S R N G G Q A S S S P N Y E G P L H S L Q S R I E D R L E R L D D A I H V L R N H A V G P S T A M P G G H G D M H G I I G P S H N G A M G G L G S G Y G T G L L S A N R H S L M V G T H R E D G V A L R G S H S L L P N Q V P V P Q L P V Q S A T S P D L N P P Q D P Y R G M P P G L Q G Q S V S S G S S E I K S D D E G D E N L Q D T K S S E D K K L D D D K K D I K S I T S N N D D E D L T P E Q K A E R E K E R R M A N N A R E R L R V R D I N E A F K E L G R M V Q L H L K S D K P Q T K L L I L H Q A V A V I L S L E Q Q V R E R N L N P K A A C L K R R E E E K V S S E P P P L S L A G P H P G M G D A S N H M G Q M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.
Alternative names
BHLHB19, ITF2, SEF2, TCF4, Transcription factor 4, TCF-4, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2, bHLHb19, ITF-2, SEF-2
Recommended products
Recombinant Human TCF-4 protein is a Human Fragment protein, in the 1 to 507 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P15884-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M K F K Q C R C S D T G L C C L D H E G K A E V Y A P S A S T A D Y N R D S P G Y P S S K P A T S T F P S S F F M Q D G H H S S D P W S S S S G M N Q P G Y A G M L G N S S H I P Q S S S Y C S L H P H E R L S Y P S H S S A D I N S S L P P M S T F H R S G T N H Y S T S S C T P P A N G T D S I M A N R G S G A A G S S Q T G D A L G K A L A S I Y S P D H T N N S F S S N P S T P V G S P P S L S A G T A V W S R N G G Q A S S S P N Y E G P L H S L Q S R I E D R L E R L D D A I H V L R N H A V G P S T A M P G G H G D M H G I I G P S H N G A M G G L G S G Y G T G L L S A N R H S L M V G T H R E D G V A L R G S H S L L P N Q V P V P Q L P V Q S A T S P D L N P P Q D P Y R G M P P G L Q G Q S V S S G S S E I K S D D E G D E N L Q D T K S S E D K K L D D D K K D I K S I T S N N D D E D L T P E Q K A E R E K E R R M A N N A R E R L R V R D I N E A F K E L G R M V Q L H L K S D K P Q T K L L I L H Q A V A V I L S L E Q Q V R E R N L N P K A A C L K R R E E E K V S S E P P P L S L A G P H P G M G D A S N H M G Q M
- Accession
- P15884
- Protein length
- Fragment
- Predicted molecular weight
- 56.6 kDa
- Amino acids
- 1 to 507
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab177595 was purified using conventional chromatography.
General info
- Function
Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The transcription factor 4 (TCF-4) also known as TCF7L2 is an important regulator of transcriptional activity. This protein weighs approximately 68 kDa and is widely expressed in multiple tissues including the pancreas intestine and brain. Through its function as a transcription factor TCF-4 binds to specific DNA sequences influencing the expression of target genes. The regulation it provides is essential for various biological processes especially during developmental stages.
- Biological function summary
TCF-4 plays an important role in Wnt signaling a pathway critical for cell growth and differentiation. It partners with β-catenin to form a functional complex enabling it to activate gene expression. This interaction influences cellular processes such as cell cycle regulation fate determination and maintenance of stem cell populations. As such TCF-4 serves an important role in regulating a balance between proliferation and differentiation in developing tissues.
- Pathways
TCF-4 is an important component within the canonical Wnt/β-catenin signaling pathway. This pathway is pivotal in embryogenesis and is also involved in the control of cell communication processes. TCF-4 interacts with other pathway members such as the APC protein which are essential for stabilizing β-catenin enabling successful transcriptional regulation. Its activity connects it chemically to the TCF/LEF family of proteins which all participate actively in gene transcription related to Wnt signaling.
- Associated diseases and disorders
TCF-4 has been implicated in type 2 diabetes and certain forms of cancer. Research shows that mutations in the TCF7L2 gene alter its function leading to increased susceptibility to type 2 diabetes emphasizing its role in glucose metabolism. Moreover dysregulation of the Wnt signaling pathway in which TCF-4 is an actor has been associated with cancer pathogenesis including colorectal cancer. TCF-4 connects closely with β-catenin and APC in these conditions highlighting its involvement in tumorigenic processes when its regulation fails.
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SDS-PAGE - Recombinant Human TCF-4 protein (ab177595)
15% SDS-PAGE analysis of 3μg ab177595.
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