Recombinant Human TCF-4/TCF7L2 protein is a Human Full Length protein, in the 1 to 455 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M P Q L N G G G G D D L G A N D E L I S F K D E G E Q E E K S S E N S S A E R D L A D V K S S L V N E S E T N Q N S S S D S E A E R R P P P R S E S F R D K S R E S L E E A A K R Q D G G L F K G P P Y P G Y P F I M I P D L T S P Y L P N G S L S P T A R T Y L Q M K W P L L D V Q A G S L Q S R Q A L K D A R S P S P A H I V S N K V P V V Q H P H H V H P L T P L I T Y S N E H F T P G N P P P H L P A D V D P K T G I P R P P H P P D I S P Y Y P L S P G T V G Q I P H P L G W Q G Q P V Y P I T T G G F R H P Y P T A L T V N A S M S R F P P H M V P P H H T L H T T G I P H P A I V T P T V K Q E S S Q S D V G S L H S S K H Q D S K K E E E K K K P H I K K P P N A F M L Y M K E M R A K V V A E C T L K E S A A I N Q I L G R R W H A L S R E E Q A K Y Y E L A R K E R Q L H M Q L Y P G W S A R D N Y G K K K K R K R D K Q P G E T N E H S E C F L N P C L S L P P I T G E K K S A F A T Y K V K A A A S A H P L Q M E A Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as a repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
Alternative names
TCF4, TCF7L2, Transcription factor 7-like 2, HMG box transcription factor 4, T-cell-specific transcription factor 4, T-cell factor 4, TCF-4, hTCF-4
Recommended products
Recombinant Human TCF-4/TCF7L2 protein is a Human Full Length protein, in the 1 to 455 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9NQB0-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M P Q L N G G G G D D L G A N D E L I S F K D E G E Q E E K S S E N S S A E R D L A D V K S S L V N E S E T N Q N S S S D S E A E R R P P P R S E S F R D K S R E S L E E A A K R Q D G G L F K G P P Y P G Y P F I M I P D L T S P Y L P N G S L S P T A R T Y L Q M K W P L L D V Q A G S L Q S R Q A L K D A R S P S P A H I V S N K V P V V Q H P H H V H P L T P L I T Y S N E H F T P G N P P P H L P A D V D P K T G I P R P P H P P D I S P Y Y P L S P G T V G Q I P H P L G W Q G Q P V Y P I T T G G F R H P Y P T A L T V N A S M S R F P P H M V P P H H T L H T T G I P H P A I V T P T V K Q E S S Q S D V G S L H S S K H Q D S K K E E E K K K P H I K K P P N A F M L Y M K E M R A K V V A E C T L K E S A A I N Q I L G R R W H A L S R E E Q A K Y Y E L A R K E R Q L H M Q L Y P G W S A R D N Y G K K K K R K R D K Q P G E T N E H S E C F L N P C L S L P P I T G E K K S A F A T Y K V K A A A S A H P L Q M E A Y
- Accession
- Q9NQB0
- Protein length
- Full Length
- Amino acids
- 1 to 455
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as a repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
- Sequence similarities
Belongs to the TCF/LEF family.
- Post-translational modifications
In vitro, phosphorylated by TNIK.
- Subcellular localisation
- Nucleus, PML body
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TCF-4 also known as TCF7L2 is a transcription factor that plays an important mechanical role in the Wnt signaling pathway. It is a protein with a molecular mass of approximately 66 kDa and is expressed in many tissues including the intestine pancreas and brain. TCF-4 acts by binding to DNA at specific sites and regulating the transcription of target genes. This transcription regulation occurs when TCF-4 partners with beta-catenin altering gene expression in response to Wnt signals.
- Biological function summary
TCF-4 influences processes like cell proliferation differentiation and apoptosis. It functions as part of a transcriptional complex with members such as beta-catenin and other cofactors which are essential for its regulatory activities. Through its control of gene expression TCF-4 affects tissue homeostasis and has implications in embryonic development. It modulates the expression of genes that are involved in important cellular processes making it a significant player in maintaining cellular equilibrium.
- Pathways
TCF-4 is essential in the Wnt/beta-catenin signaling pathway a critical pathway that regulates cell fate and patterning during development. This pathway involves the accumulation of beta-catenin in the cytoplasm and its subsequent translocation into the nucleus. In the nucleus beta-catenin partners with TCF-4 to regulate gene transcription. Additionally TCF-4 interacts with other pathway proteins such as LEF1 (Lymphoid Enhancer-Binding Factor 1) to modulate Wnt target genes illustrating its integration within complex signaling networks.
- Associated diseases and disorders
TCF-4 has strong connections to type 2 diabetes and colorectal cancer. Variations in the TCF7L2 gene have been linked to increased risk of type 2 diabetes potentially due to its role in glucose metabolism. In colorectal cancer aberrant activation of the Wnt signaling pathway often involving TCF-4 and beta-catenin leads to uncontrolled cell growth. Understanding these connections can provide insights into the pathogenesis of these diseases and inform treatment strategies targeting TCF-4 and its associated pathways.
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1 product image
SDS-PAGE - Recombinant Human TCF-4/TCF7L2 protein (ab159648)
ab159648 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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