Recombinant Human TCF12 protein is a Human Fragment protein, in the 364 to 453 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
V G S P S P L T G T S Q W P R P G G Q A P S S P S Y E N S L H S L K N R V E Q Q L H E H L Q D A M S F L K D V C E Q S R M E D R L D R L D D A I H V L R N H A V G P S T S L P A G H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3') (By similarity). May be involved in the functional network that regulates the development of the GnRH axis (PubMed:32620954).
Alternative names
BHLHB20, HEB, HTF4, TCF12, Transcription factor 12, TCF-12, Class B basic helix-loop-helix protein 20, DNA-binding protein HTF4, E-box-binding protein, Transcription factor HTF-4, bHLHb20
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Recombinant Human TCF12 protein is a Human Fragment protein, in the 364 to 453 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q99081-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- V G S P S P L T G T S Q W P R P G G Q A P S S P S Y E N S L H S L K N R V E Q Q L H E H L Q D A M S F L K D V C E Q S R M E D R L D R L D D A I H V L R N H A V G P S T S L P A G H
- Accession
- Q99081
- Protein length
- Fragment
- Predicted molecular weight
- 35.64 kDa
- Amino acids
- 364 to 453
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3') (By similarity). May be involved in the functional network that regulates the development of the GnRH axis (PubMed:32620954).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TCF12 also known as transcription factor 12 or Heb functions as a basic helix-loop-helix (bHLH) transcription factor. It plays roles in DNA binding and regulation of gene expression. The protein has a molecular mass of approximately 75 kDa. TCF12 expresses in various tissues with notable levels observed in the brain and skeletal muscle. It influences the development and differentiation of different cell types due to its transcriptional regulation activities.
- Biological function summary
TCF12 controls the transcription of genes involved in cellular differentiation proliferation and development. It frequently functions as part of heterodimeric complexes with other bHLH proteins such as E2A. These complexes allow TCF12 to regulate a variety of target genes involved in tissue development and cell fate decisions. TCF12's ability to form these complexes enhances its functional diversity and impact on biological processes.
- Pathways
The role of TCF12 extends to the Notch signaling pathway where it interacts with proteins like Notch receptors to affect cell fate determination. The protein also participates in the Myogenesis pathway collaborating with MyoD to facilitate the differentiation of myoblasts into mature muscle fibers. These pathways highlight TCF12's involvement in developmental processes and lineage specification.
- Associated diseases and disorders
TCF12 mutations and dysregulation have associations with craniosynostosis a condition characterized by premature fusion of cranial sutures. TCF12 also relates to certain types of cancer due to its regulatory effects on cell proliferation. In these contexts TCF12 may interact with proteins like TWIST1 which also influences developmental and tumorigenic processes highlighting potential therapeutic targets for intervention.
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1 product image
SDS-PAGE - Recombinant Human TCF12 protein (ab152729)
12.5% SDS-PAGE analysis of ab152729 stained with Coomassie Blue.
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Product protocols
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