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AB160939

Recombinant Human TCIRG1 protein (GST tag N-Terminus)

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Recombinant Human TCIRG1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 614 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

ATP6N1C, ATP6V0A3, TCIRG1, V-type proton ATPase 116 kDa subunit a 3, V-ATPase 116 kDa subunit a 3, Osteoclastic proton pump 116 kDa subunit, T-cell immune regulator 1, T-cell immune response cDNA7 protein, Vacuolar proton translocating ATPase 116 kDa subunit a isoform 3, OC-116 kDa, OC116, TIRC7

1 Images
SDS-PAGE - Recombinant Human TCIRG1 protein (GST tag N-Terminus) (AB160939)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human TCIRG1 protein (GST tag N-Terminus) (AB160939)

ab160939 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q13488

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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SDS-PAGE - Recombinant Human ZP2 protein (GST tag N-Terminus) (AB159886)

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Primary Antibodies

AB139812

Anti-TCIRG1 antibody

Western blot - Anti-TCIRG1 antibody (AB139812)

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AB162402

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SDS-PAGE - Recombinant Human Zinc finger protein 691 (GST tag N-Terminus) (AB162402)

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AB161146

Recombinant Human BS69 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human BS69 protein (GST tag N-Terminus) (AB161146)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MTFLISYWGEQIGQKIRKITDCFHCHVFPFLQQEEARLGALQQLQQQSQELQEVLGETERFLSQVLGRVLQLLPPGQVQVHKMKAVYLALNQCSVSTTHKCLIAEAWCSVRDLPALQEALRDSSMEEGVSAVAHRIPCRDMPPTLIRTNRFTASFQGIVDAYGVGRYQEVNPAPYTIITFPFLFAVMFGDVGHGLLMFLFALAMVLAENRPAVKAAQNEIWQTFFRGRYLLLLMGLFSIYTGFIYNECFSRATSIFPSGWSVAAMANQSGWSDAFLAQHTMLTLDPNVTGVFLGPYPFGIDPIWSLAANHLSFLNSFKMKMSVILGVVHMAFGVVLGVFNHVHFGQRHRLLLETLPELTFLLGLFGYLVFLVIYKWLCVWAARAASAPSILIHFINMFLFSHSPSNRLLYPRQEVVQATLVVLALAMVPILLLGTPLHLLHRHRRRLRRRPADRQEENKAGLLDLPDASVNGWSSDEEKAGGLDDEEEAELVPSEVLMHQAIHTIEFCLGCVSNTASYLRLWALSLAHAQLSEVLWAMVMRIGLGLGREVGVAAVVLVPIFAAFAVMTVAILLVMEGLSAFLHALRLHWVEFQNKFYSGTGYKLSPFTFAATDD","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":614,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q13488","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCIRG1 also known as T-cell immune regulator 1 or ATP6V0A3 functions as a component of the vacuolar-type H+-ATPase (V-ATPase) complex. This protein which has a mass of approximately 116 kDa acts by facilitating proton translocation across membranes important for cellular pH regulation. TCIRG1 is expressed mainly in osteoclasts and other cells with high endocytosis rates like renal epithelial cells and macrophages. It contributes to acidification necessary for protein degradation and nutrient uptake.
Biological function summary

The protein participates in bone resorption by osteoclasts. It forms part of the V-ATPase complex that is composed of several subunits coordinating with these subunits to pump protons across the cell membrane. This action aids in creating an acidic microenvironment essential for dissolving bone mineral matrices. TCIRG1 is instrumental in maintaining the acidic conditions required for various cellular processes including lysosomal digestion and neurotransmitter release.

Pathways

TCIRG1 is significantly involved in the lysosomal degradation pathway and bone remodeling process. It partners with proteins like VATP1 and ATP6V1C1 to efficiently drive the proton pump activity. Through its role in bone resorption TCIRG1 integrates into the RANK/RANKL signaling pathway where it assists in the downstream effects of osteoclast differentiation and activity. This involvement is important for maintaining calcium homeostasis and skeletal integrity.

Mutations in TCIRG1 lead to autosomal recessive osteopetrosis a condition characterized by impaired bone resorption due to defective osteoclast function. This disorder results from the failure to create the acidic environment required for bone matrix degradation. Another condition associated with TCIRG1 includes renal tubular acidosis a result of disrupted acid-base balance in kidney function. The RANK protein interacts within these disease contexts influencing osteoclast activity and bone density manifestations.
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Specifications

Form

Liquid

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General info

Function

Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (By similarity). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). Seems to be directly involved in T-cell activation (PubMed : 10329006).

Sequence similarities

Belongs to the V-ATPase 116 kDa subunit family.

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Product protocols

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Target data

Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (By similarity). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). Seems to be directly involved in T-cell activation (PubMed : 10329006).
See full target information TCIRG1
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