JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB101043

Recombinant Human TCP1 alpha/CCTA protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human TCP1 alpha/CCTA protein is a Human Full Length protein, in the 1 to 576 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

CCT1, CCTA, TCP1, T-complex protein 1 subunit alpha, TCP-1-alpha, CCT-alpha, Chaperonin containing T-complex polypeptide 1 subunit 1

1 Images
SDS-PAGE - Recombinant Human TCP1 alpha/CCTA protein (AB101043)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TCP1 alpha/CCTA protein (AB101043)

ab101043 at 3 µg being analysed by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P17987

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

This product was previously labelled as TCP1 alpha
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMEGPLSVFGDRSTGETIRSQNVMAAASIANIVKSSLGPVGLDKMLVDDIGDVTITNDGATILKLLEVEHPAAKVLCELADLQDKEVGDGTTSVVIIAAELLKNADELVKQKIHPTSVISGYRLACKEAVRYINENLIVNTDELGRDCLINAAKTSMSSKIIGINGDFFANMVVDAVLAIKYTDIRGQPRYPVNSVNILKAHGRSQMESMLISGYALNCVVGSQGMPKRIVNAKIACLDFSLQKTKMKLGVQVVITDPEKLDQIRQRESDITKERIQKILATGANVILTTGGIDDMCLKYFVEAGAMAVRRVLKRDLKRIAKASGATILSTLANLEGEETFEAAMLGQAEEVVQERICDDELILIKNTKARTSASIILRGANDFMCDEMERSLHDALCVVKRVLESKSVVPGGGAVEAALSIYLENYATSMGSREQLAIAEFARSLLVIPNTLAVNAAQDSTDLVAKLRAFHNEAQVNPERKNLKWIGLDLSNGKPRDNKQAGVFEPTIVKVKSLKFATEAAITILRIDDLIKLHPESKDDKHGSYEDAVHSGALND","proteinLength":"Full Length","predictedMolecularWeight":"62.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":576,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P17987","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCP1 alpha also known as CCTA or alpha TCP functions as a molecular chaperone within the cell. It assists in the folding of newly synthesized proteins specifically in large complexes. TCP1 alpha is one of eight subunits that form the chaperonin-containing TCP1 (CCT) complex weighing around 60 kDa. Researchers find TCP1 alpha in high concentrations in the cytoplasm expressing abundantly in tissues with high rates of protein synthesis such as testis and brain.
Biological function summary

The CCT complex which includes TCP1 alpha plays a significant role in maintaining cellular proteostasis by ensuring correct protein folding. It is essential in the folding of actin and tubulin both critical proteins for cytoskeletal functions. TCP1 alpha as part of the CCT complex prevents aggregation of polypeptides maintaining them in a functional state. By doing so TCP1 alpha influences numerous cellular processes including cell division and cytoskeletal arrangements.

Pathways

TCP1 alpha participates in essential cellular pathways such as the cytoskeleton organization and protein folding pathways. In cytoskeleton organization it ensures the correct folding of actin and tubulin which are important components for cytoskeletal integrity. This target also collaborates with heat shock proteins in protein folding pathways acting as a checkpoint to ensure only properly folded proteins advance through the pathway. Through these pathways TCP1 alpha keeps cellular structures and functions in check.

The malfunction of TCP1 alpha within the CCT complex has connections to neurodegenerative diseases and cancer. Aberrations in protein folding can lead to aggregations implicated in neurodegenerative disorders like Huntington's disease. Additionally the link between TCP1 alpha and imbalanced protein synthesis suggests its involvement in cancer cell proliferation where chaperone activity is upregulated. The interaction of TCP1 alpha with proteins like p53 a critical regulator in cancer highlights its role in disease development and progression.
style
left-column

Specifications

Form

Liquid

Additional notes

purified by using conventional chromatography.

style
left-column

General info

Function

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed : 25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed : 20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).

Sequence similarities

Belongs to the TCP-1 chaperonin family.

Subcellular localisation

Cytoskeleton

style
left-column

Product protocols

style
left-column

Target data

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed : 25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed : 20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).
See full target information TCP1
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB99263

Recombinant Human Tryptophanyl tRNA synthetase/WRS protein

SDS-PAGE - Recombinant Human Tryptophanyl tRNA synthetase/WRS protein (AB99263)

  • 0
  • 0
Proteins & Peptides

AB95890

Recombinant Human PC4 protein

SDS-PAGE - Recombinant Human PC4 protein (AB95890)

  • 0
  • 0
Primary Antibodies

AB92587

Anti-TCP1 alpha/CCTA antibody [EPR4081]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 alpha/CCTA antibody [EPR4081] (AB92587)

  • 0
  • 0
Proteins & Peptides

AB98233

Recombinant Human THTPA protein

SDS-PAGE - Recombinant Human THTPA protein (AB98233)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com