Recombinant Human TCP1 epsilon/CCT5 protein is a Human Full Length protein, in the 1 to 541 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
Wheat germ
- Tags
Tag free
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
Amino acid sequence
M A S M G T L A F D E Y G R P F L I I K D Q D R K S R L M G L E A L K S H I M A A K A V A N T M R T S L G P N G L D K M M V D K D G D V T V T N D G A T I L S M M D V D H Q I A K L M V E L S K S Q D D E I G D G T T G V V V L A G A L L E E A E Q L L D R G I H P I R I A D G Y E Q A A R V A I E H L D K I S D S V L V D I K D T E P L I Q T A K T T L G S K V V N S C H R Q M A E I A V N A V L T V A D M E R R D V D F E L I K V E G K V G G R L E D T K L I K G V I V D K D F S H P Q M P K K V E D A K I A I L T C P F E P P K P K T K H K L D V T S V E D Y K A L Q K Y E K E K F E E M I Q Q I K E T G A N L A I C Q W G F D D E A N H L L L Q N N L P A V R W V G G P E I E L I A I A T G G R I V P R F S E L T A E K L G F A G L V Q E I S F G T T K D K M L V I E Q C K N S R A V T I F I R G G N K M I I E E A K R S L H D A L C V I R N L I R D N R V V Y G G G A A E I S C A L A V S Q E A D K C P T L E Q Y A M R A F A D A L E V I P M A L S E N S G M N P I Q T M T E V R A R Q V K E M N P A L G I D C L H K G T N D M K Q Q H V I E T L I G K K Q Q I S L A T Q M V R M I L K I D D I R K P G E S E E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).
Alternative names
T-complex protein 1 subunit epsilon, TCP-1-epsilon, CCT-epsilon, KIAA0098, CCTE, CCT5
Recommended products
Recombinant Human TCP1 epsilon/CCT5 protein is a Human Full Length protein, in the 1 to 541 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
Wheat germ
- Tags
Tag free
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
- Accession
- P48643-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
M A S M G T L A F D E Y G R P F L I I K D Q D R K S R L M G L E A L K S H I M A A K A V A N T M R T S L G P N G L D K M M V D K D G D V T V T N D G A T I L S M M D V D H Q I A K L M V E L S K S Q D D E I G D G T T G V V V L A G A L L E E A E Q L L D R G I H P I R I A D G Y E Q A A R V A I E H L D K I S D S V L V D I K D T E P L I Q T A K T T L G S K V V N S C H R Q M A E I A V N A V L T V A D M E R R D V D F E L I K V E G K V G G R L E D T K L I K G V I V D K D F S H P Q M P K K V E D A K I A I L T C P F E P P K P K T K H K L D V T S V E D Y K A L Q K Y E K E K F E E M I Q Q I K E T G A N L A I C Q W G F D D E A N H L L L Q N N L P A V R W V G G P E I E L I A I A T G G R I V P R F S E L T A E K L G F A G L V Q E I S F G T T K D K M L V I E Q C K N S R A V T I F I R G G N K M I I E E A K R S L H D A L C V I R N L I R D N R V V Y G G G A A E I S C A L A V S Q E A D K C P T L E Q Y A M R A F A D A L E V I P M A L S E N S G M N P I Q T M T E V R A R Q V K E M N P A L G I D C L H K G T N D M K Q Q H V I E T L I G K K Q Q I S L A T Q M V R M I L K I D D I R K P G E S E E
- Accession
- Protein length
Full Length
- Predicted molecular weight
85.25 kDa
- Amino acids
1 to 541
- Nature
Recombinant
Specifications
- Form
Liquid
General info
- Function
Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).
- Sequence similarities
Belongs to the TCP-1 chaperonin family.
- Subcellular localisation
Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product was previously labelled as TCP1 epsilon.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TCP1 epsilon also known as CCT5 is an important component of the chaperonin-containing TCP1 complex. This protein weighs around 61 kDa and is expressed in a variety of tissues with higher prevalence in areas with significant protein synthesis and folding activities like the brain and testis. Mechanically TCP1 epsilon facilitates the folding of newly synthesized and stress-denatured proteins by providing an optimal environment for proper protein conformation. It does this by assembling into a barrel-shaped structure that encapsulates the substrate proteins.
- Biological function summary
CCT5 is part of the cytosolic chaperonin complex called the TRiC (TCP1 Ring Complex) which is essential for the folding of actin and tubulin. This complex ensures that cytoskeletal proteins achieve their correct tertiary structures necessary for maintaining cellular architecture and function. Additionally this complex prevents the misfolding and aggregation of proteins a process that helps maintain cellular homeostasis and prevent cellular stress.
- Pathways
CCT5 plays a significant role in the cytoskeleton modeling and protein homeostasis pathways. In the cytoskeleton pathway CCT5's interaction with actin and tubulin is vital ensuring these proteins attain functional conformation. In the protein homeostasis pathway CCT5 assists in degrading misfolded proteins working closely with other components like heat shock proteins (HSPs) to manage cellular protein load effectively. This interaction highlights its importance in preventing the accumulation of damaged proteins.
- Associated diseases and disorders
Mutations in CCT5 have been linked to some neurodegenerative conditions like Hereditary Sensory Neuropathy Type 2 (HSN2). The failure of CCT5 to assist in proper protein folding can lead to neuronal damage owing to protein aggregates. Additionally disruptions in CCT5 are associated with some forms of cancer where abnormal protein folding influences cell proliferation. CCT5 work in conjunction with other proteins like p53 to maintain cellular function and dysregulation in cancer highlights its role in cellular growth and apoptosis regulation.
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1 product image
SDS-PAGE - Recombinant Human TCP1 epsilon/CCT5 protein (ab132283)
12.5% SDS-PAGE stained with Coomassie Blue showing ab132283.
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