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AB132283

Recombinant Human TCP1 epsilon/CCT5 protein (GST tag N-Terminus)

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Recombinant Human TCP1 epsilon/CCT5 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 541 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

CCTE, KIAA0098, CCT5, T-complex protein 1 subunit epsilon, TCP-1-epsilon, CCT-epsilon, Chaperonin containing T-complex polypeptide 1 subunit 5

1 Images
SDS-PAGE - Recombinant Human TCP1 epsilon/CCT5 protein (GST tag N-Terminus) (AB132283)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human TCP1 epsilon/CCT5 protein (GST tag N-Terminus) (AB132283)

12.5% SDS-PAGE stained with Coomassie Blue showing ab132283.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

P48643

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

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Recombinant Human TCP1 theta protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human TCP1 theta protein (GST tag N-Terminus) (AB161115)

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Primary Antibodies

AB129016

Anti-TCP1 epsilon/CCT5 antibody [EPR7562]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (AB129016)

  • 5
  • 1
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Recombinant Human DNAJB2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human DNAJB2 protein (His tag N-Terminus) (AB101215)

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Proteins & Peptides

AB101203

Recombinant Human HMGE protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human HMGE protein (His tag N-Terminus) (AB101203)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as TCP1 epsilon.
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Sequence info

[{"sequence":"MASMGTLAFDEYGRPFLIIKDQDRKSRLMGLEALKSHIMAAKAVANTMRTSLGPNGLDKMMVDKDGDVTVTNDGATILSMMDVDHQIAKLMVELSKSQDDEIGDGTTGVVVLAGALLEEAEQLLDRGIHPIRIADGYEQAARVAIEHLDKISDSVLVDIKDTEPLIQTAKTTLGSKVVNSCHRQMAEIAVNAVLTVADMERRDVDFELIKVEGKVGGRLEDTKLIKGVIVDKDFSHPQMPKKVEDAKIAILTCPFEPPKPKTKHKLDVTSVEDYKALQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIAIATGGRIVPRFSELTAEKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNKMIIEEAKRSLHDALCVIRNLIRDNRVVYGGGAAEISCALAVSQEADKCPTLEQYAMRAFADALEVIPMALSENSGMNPIQTMTEVRARQVKEMNPALGIDCLHKGTNDMKQQHVIETLIGKKQQISLATQMVRMILKIDDIRKPGESEE","proteinLength":"Full Length","predictedMolecularWeight":"85.25 kDa","actualMolecularWeight":null,"aminoAcidEnd":541,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P48643","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCP1 epsilon also known as CCT5 is an important component of the chaperonin-containing TCP1 complex. This protein weighs around 61 kDa and is expressed in a variety of tissues with higher prevalence in areas with significant protein synthesis and folding activities like the brain and testis. Mechanically TCP1 epsilon facilitates the folding of newly synthesized and stress-denatured proteins by providing an optimal environment for proper protein conformation. It does this by assembling into a barrel-shaped structure that encapsulates the substrate proteins.
Biological function summary

CCT5 is part of the cytosolic chaperonin complex called the TRiC (TCP1 Ring Complex) which is essential for the folding of actin and tubulin. This complex ensures that cytoskeletal proteins achieve their correct tertiary structures necessary for maintaining cellular architecture and function. Additionally this complex prevents the misfolding and aggregation of proteins a process that helps maintain cellular homeostasis and prevent cellular stress.

Pathways

CCT5 plays a significant role in the cytoskeleton modeling and protein homeostasis pathways. In the cytoskeleton pathway CCT5's interaction with actin and tubulin is vital ensuring these proteins attain functional conformation. In the protein homeostasis pathway CCT5 assists in degrading misfolded proteins working closely with other components like heat shock proteins (HSPs) to manage cellular protein load effectively. This interaction highlights its importance in preventing the accumulation of damaged proteins.

Mutations in CCT5 have been linked to some neurodegenerative conditions like Hereditary Sensory Neuropathy Type 2 (HSN2). The failure of CCT5 to assist in proper protein folding can lead to neuronal damage owing to protein aggregates. Additionally disruptions in CCT5 are associated with some forms of cancer where abnormal protein folding influences cell proliferation. CCT5 work in conjunction with other proteins like p53 to maintain cellular function and dysregulation in cancer highlights its role in cellular growth and apoptosis regulation.
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Specifications

Form

Liquid

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General info

Function

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed : 25467444, PubMed : 36493755, PubMed : 35449234, PubMed : 37193829). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed : 20080638).

Sequence similarities

Belongs to the TCP-1 chaperonin family.

Post-translational modifications

Ubiquitinated by the DCX(DCAF12) complex specifically recognizes the diglutamate (Glu-Glu) at the C-terminus, leading to its degradation.

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Product protocols

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Target data

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed : 25467444, PubMed : 36493755, PubMed : 35449234, PubMed : 37193829). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed : 20080638).
See full target information CCT5
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