Recombinant Human TDGF1 Protein is a Human Full Length protein, in the 31 to 172 aa range, expressed in HEK 293, with >=95% purity, >= 0.005 EU/µg endotoxin level and suitable for HPLC, MS.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- >= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, MS
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
Amino acid sequence
L G H Q E F A R P S R G Y L A F R D D S I W P Q E E P A I R P R S S Q R V P P M G I Q H S K E L N R T C C L N G G T C M L G S F C A C P P S F Y G R N C E H D V R K E N C G S V P H D T W L P K K C S L C K C W H G Q L R C F P Q A F L P G C D G L V M D E H L V A S R T P E L P P S A R T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by TMEM8A modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells (PubMed:27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed:11909953).
Alternative names
CRIPTO-1, TDGF1, CRIPTO, Protein Cripto, Cripto-1 growth factor, Epidermal growth factor-like cripto protein CR1, Teratocarcinoma-derived growth factor 1, CRGF
Recommended products
Recombinant Human TDGF1 Protein is a Human Full Length protein, in the 31 to 172 aa range, expressed in HEK 293, with >=95% purity, >= 0.005 EU/µg endotoxin level and suitable for HPLC, MS.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- >= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, MS
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
- Accession
- P13385-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- L G H Q E F A R P S R G Y L A F R D D S I W P Q E E P A I R P R S S Q R V P P M G I Q H S K E L N R T C C L N G G T C M L G S F C A C P P S F Y G R N C E H D V R K E N C G S V P H D T W L P K K C S L C K C W H G Q L R C F P Q A F L P G C D G L V M D E H L V A S R T P E L P P S A R T
- Accession
- P13385
- Protein length
- Full Length
- Predicted molecular weight
- 16 kDa
- Actual molecular weight
- 16.15 kDa
- Amino acids
- 31 to 172
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by TMEM8A modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells (PubMed:27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed:11909953).
- Sequence similarities
Belongs to the EGF-CFC (Cripto-1/FRL1/Cryptic) family.
- Post-translational modifications
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, it is processed by GPI processing phospholipase A2 (TMEM8A), removing an acyl-chain at the sn-2 position of GPI and releasing CRIPTO as a lysophosphatidylinositol-bearing form, which is further cleaved by phospholipase D (GPLD1) into a soluble form.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Mass Spectrometry - Recombinant Human TDGF1 Protein (ab314699)
Mass determination by ESI-TOF.
Predicted MW is 16000.00 Da (+/-10 Da by ESI-TOF). Observed MW is 16146.55
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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