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AB314699

Recombinant Human TDGF1 Protein

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Recombinant Human TDGF1 Protein is a Human Full Length protein, in the 31 to 172 aa range, expressed in HEK 293 cells, with >95%, >0.005 EU/µg endotoxin level, suitable for HPLC, Mass Spec.

View Alternative Names

CRIPTO-1, TDGF1, CRIPTO, Protein Cripto, Cripto-1 growth factor, Epidermal growth factor-like cripto protein CR1, Teratocarcinoma-derived growth factor 1, CRGF

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Mass Spectrometry - Recombinant Human TDGF1 Protein (AB314699)
  • Mass Spec

Lab

Mass Spectrometry - Recombinant Human TDGF1 Protein (AB314699)

Mass determination by ESI-TOF. Predicted MW is 16000.00 Da (+/-10 Da by ESI-TOF). Observed MW is 16146.55

HPLC - Recombinant Human TDGF1 Protein (AB314699)
  • HPLC

Supplier Data

HPLC - Recombinant Human TDGF1 Protein (AB314699)

HPLC analysis of ab314699

Key facts

Purity

>95% HPLC

Endotoxin level

>0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

HPLC, Mass Spec

applications

Biologically active

No

Mass Spectrometry

LC-MS/MS

Accession

P13385

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"LGHQEFARPSRGYLAFRDDSIWPQEEPAIRPRSSQRVPPMGIQHSKELNRTCCLNGGTCMLGSFCACPPSFYGRNCEHDVRKENCGSVPHDTWLPKKCSLCKCWHGQLRCFPQAFLPGCDGLVMDEHLVASRTPELPPSART","proteinLength":"Full Length","predictedMolecularWeight":"16 kDa","actualMolecularWeight":"16.15 kDa","aminoAcidEnd":172,"aminoAcidStart":31,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P13385","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
False
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Specifications

Form

Lyophilized

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General info

Function

GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by TMEM8A modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells (PubMed : 27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed : 11909953).

Sequence similarities

Belongs to the EGF-CFC (Cripto-1/FRL1/Cryptic) family.

Post-translational modifications

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, it is processed by GPI processing phospholipase A2 (TMEM8A), removing an acyl-chain at the sn-2 position of GPI and releasing CRIPTO as a lysophosphatidylinositol-bearing form, which is further cleaved by phospholipase D (GPLD1) into a soluble form.

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Product protocols

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Target data

GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by TMEM8A modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells (PubMed : 27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed : 11909953).
See full target information CRIPTO
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