Recombinant Human TEAD4 protein (His tag) is a Human Fragment protein, in the 74 to 434 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M Y G R N E L I A R Y I K L R T G K T R T R K Q V S S H I Q V L A R R K A R E I Q A K L K D Q A A K D K A L Q S M A A M S S A Q I I S A T A F H S S M A L A R G P G R P A V S G F W Q G A L P G Q A G T S H D V K P F S Q Q T Y A V Q P P L P L P G F E S P A G P A P S P S A P P A P P W Q G R S V A S S K L W M L E F S A F L E Q Q Q D P D T Y N K H L F V H I G Q S S P S Y S D P Y L E A V D I R Q I Y D K F P E K K G G L K D L F E R G P S N A F F L V K F W A D L N T N I E D E G S S F Y G V S S Q Y E S P E N M I I T C S T K V C S F G K Q V V E K V E T E Y A R Y E N G H Y S Y R I H R S P L C E Y M I N F I H K L K H L P E K Y M M N S V L E N F T I L Q V V T N R D T Q E T L L C I A Y V F E V S A S E H G A Q H H I Y R L V K E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.
Alternative names
RTEF1, TCF13L1, TEF3, TEAD4, Transcriptional enhancer factor TEF-3, TEA domain family member 4, Transcription factor 13-like 1, Transcription factor RTEF-1, TEAD-4
Recommended products
Recombinant Human TEAD4 protein (His tag) is a Human Fragment protein, in the 74 to 434 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q15561-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M Y G R N E L I A R Y I K L R T G K T R T R K Q V S S H I Q V L A R R K A R E I Q A K L K D Q A A K D K A L Q S M A A M S S A Q I I S A T A F H S S M A L A R G P G R P A V S G F W Q G A L P G Q A G T S H D V K P F S Q Q T Y A V Q P P L P L P G F E S P A G P A P S P S A P P A P P W Q G R S V A S S K L W M L E F S A F L E Q Q Q D P D T Y N K H L F V H I G Q S S P S Y S D P Y L E A V D I R Q I Y D K F P E K K G G L K D L F E R G P S N A F F L V K F W A D L N T N I E D E G S S F Y G V S S Q Y E S P E N M I I T C S T K V C S F G K Q V V E K V E T E Y A R Y E N G H Y S Y R I H R S P L C E Y M I N F I H K L K H L P E K Y M M N S V L E N F T I L Q V V T N R D T Q E T L L C I A Y V F E V S A S E H G A Q H H I Y R L V K E
- Accession
- Q15561
- Protein length
- Fragment
- Predicted molecular weight
- 44.7 kDa
- Amino acids
- 74 to 434
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TEAD4 also known as TEF-5 or TEF is a transcription factor that plays a significant mechanical role in gene regulation. It belongs to the TEAD family of proteins. TEAD4 has an approximate mass of 48 kDa and is ubiquitously expressed in various tissues. It interacts with different cofactors to regulate gene expression and influences several cellular processes. The protein's expression is especially noted in cancerous tissues emphasizing its role in cellular growth and proliferation.
- Biological function summary
TEAD4 is important for embryonic development and cell fate determination. It forms a complex with YAP (Yes-associated protein) to regulate transcriptional activities. The TEAD4-YAP complex binds to DNA driving the expression of genes involved in cell proliferation and survival. TEAD4 also coordinates with _tef_1 and several other transcription factors to regulate genes necessary for development and differentiation influencing processes such as muscle and neural tissue formation.
- Pathways
TEAD4 is primarily involved in the Hippo signaling pathway known to regulate organ size by controlling cell proliferation and apoptosis. TEAD4 interacts with proteins like YAP and TAZ which play integral roles within this pathway. The Hippo pathway influences cell growth and TEAD4 as a mediator of this action contributes to the balance between cell proliferation and apoptosis. TEAD4's role extends to pathways involving cell cycle regulation and response to cellular stress.
- Associated diseases and disorders
TEAD4 exhibits significant relevance in cancer and cardiovascular diseases. Overexpression or dysregulation of TEAD4 often through its interaction with YAP is linked with tumorigenesis in breast and liver cancers. It promotes cancer cell proliferation migration and invasion. In the cardiovascular system TEAD4 impacts heart development and function with implications in congenital heart defects. Its interaction with proteins like _tef_1 also highlights its role in these diseases making it a potential therapeutic target.
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1 product image
SDS-PAGE - Recombinant Human TEAD4 protein (His tag) (ab238222)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab238222.
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