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AB105197

Recombinant human Tec protein

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Recombinant human Tec protein is a Human Full Length protein, in the 1 to 631 aa range, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

PSCTK4, TEC, Tyrosine-protein kinase Tec

4 Images
Functional Studies - Recombinant human Tec protein (AB105197)
  • FuncS

Unknown

Functional Studies - Recombinant human Tec protein (AB105197)

The specific activity of Tec (ab105197) was determined to be 8.5 nmol/min/mg as per activity assay protocol

Functional Studies - Recombinant human Tec protein (AB105197)
  • FuncS

Unknown

Functional Studies - Recombinant human Tec protein (AB105197)

The Specific activity of ab105197 was determined to be 10 nmol/min/mg.

SDS-PAGE - Recombinant human Tec protein (AB105197)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Tec protein (AB105197)

SDS-PAGE showing ab105197 at approximately 103kDa.

SDS-PAGE - Recombinant human Tec protein (AB105197)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Tec protein (AB105197)

SDS PAGE analysis of ab105197

Key facts

Purity

>75% Densitometry

Purity was determined to be >75% by densitometry. Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS, WB

applications

Biologically active

Yes

Biological activity

The Specific activity of ab105197 was determined to be 10 nmol/min/mg.

Accession

P42680

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Primary Antibodies

AB32368

Anti-Tec antibody [Y398]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Tec antibody [Y398] (AB32368)

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Primary Antibodies

AB4223

FITC Anti-C1q antibody

Immunocytochemistry - FITC Anti-C1q antibody (AB4223)

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Primary Antibodies

AB200999

Anti-C3 antibody [EPR19394]

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Western blot - Anti-C3 antibody [EPR19394] (AB200999)

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Primary Antibodies

AB198242

Anti-NDUFA6 antibody

Western blot - Anti-NDUFA6 antibody (AB198242)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"103 kDa","actualMolecularWeight":null,"aminoAcidEnd":631,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P42680","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tec also known as Tec protein tyrosine kinase functions as a non-receptor tyrosine kinase involved in signal transduction. Its molecular mass is approximately 72 kDa. Tec is expressed predominantly in hematopoietic cells such as B cells T cells and myeloid lineage cells. This protein plays a significant role in mediating cellular communication by transferring phosphate groups from ATP to specific tyrosine residues on target proteins.
Biological function summary

Tec influences cellular processes like proliferation differentiation and survival. Tec belongs to the Tec kinase family which also includes Btk Itk Rlk and Bmx. Though Tec does not form permanent complexes it interacts dynamically with various signaling proteins. These interactions allow Tec to modulate immune cell development and function significantly impacting the adaptive immune response.

Pathways

Tec is an integral part of the B-cell receptor (BCR) signaling pathway and the PI3K/Akt signaling pathway. Upon activation of BCR Tec aids in signal propagation leading to B cell activation and proliferation. Tec operates synergistically with proteins like Src family kinases leading to the activation of downstream signaling pathways. The protein also facilitates the Akt pathway which controls cell growth and survival by interacting with phosphatidylinositol 3-kinase (PI3K).

Tec links to immunodeficiencies and cancers such as leukemia. Dysfunctional Tec signaling can impair immune responses contributing to conditions like X-linked agammaglobulinemia a disorder often connected to mutations in Bruton's tyrosine kinase (Btk). Overactive Tec signaling may promote leukemogenesis linking Tec to leukemias where it cooperates with proteins such as STAT5 which is involved in cell growth and survival pathways.
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General info

Function

Non-receptor tyrosine kinase that contributes to signaling from many receptors and participates as a signal transducer in multiple downstream pathways, including regulation of the actin cytoskeleton. Plays a redundant role to ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. Required for TCR-dependent IL2 gene induction. Phosphorylates DOK1, one CD28-specific substrate, and contributes to CD28-signaling. Mediates signals that negatively regulate IL2RA expression induced by TCR cross-linking. Plays a redundant role to BTK in BCR-signaling for B-cell development and activation, especially by phosphorylating STAP1, a BCR-signaling protein. Required in mast cells for efficient cytokine production. Involved in both growth and differentiation mechanisms of myeloid cells through activation by the granulocyte colony-stimulating factor CSF3, a critical cytokine to promoting the growth, differentiation, and functional activation of myeloid cells. Participates in platelet signaling downstream of integrin activation. Cooperates with JAK2 through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. GRB10, a negative modifier of the FOS activation pathway, is another substrate of TEC. TEC is involved in G protein-coupled receptor- and integrin-mediated signalings in blood platelets. Plays a role in hepatocyte proliferation and liver regeneration and is involved in HGF-induced ERK signaling pathway. TEC also regulates FGF2 unconventional secretion (endoplasmic reticulum (ER)/Golgi-independent mechanism) under various physiological conditions through phosphorylation of FGF2 'Tyr-215'. May also be involved in the regulation of osteoclast differentiation.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.

Post-translational modifications

Following B-cell or T-cell receptors engagement, translocates to the plasma membrane where it gets phosphorylated at Tyr-519. Undergoes also tyrosine phosphorylation during platelet activation.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Non-receptor tyrosine kinase that contributes to signaling from many receptors and participates as a signal transducer in multiple downstream pathways, including regulation of the actin cytoskeleton. Plays a redundant role to ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. Required for TCR-dependent IL2 gene induction. Phosphorylates DOK1, one CD28-specific substrate, and contributes to CD28-signaling. Mediates signals that negatively regulate IL2RA expression induced by TCR cross-linking. Plays a redundant role to BTK in BCR-signaling for B-cell development and activation, especially by phosphorylating STAP1, a BCR-signaling protein. Required in mast cells for efficient cytokine production. Involved in both growth and differentiation mechanisms of myeloid cells through activation by the granulocyte colony-stimulating factor CSF3, a critical cytokine to promoting the growth, differentiation, and functional activation of myeloid cells. Participates in platelet signaling downstream of integrin activation. Cooperates with JAK2 through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. GRB10, a negative modifier of the FOS activation pathway, is another substrate of TEC. TEC is involved in G protein-coupled receptor- and integrin-mediated signalings in blood platelets. Plays a role in hepatocyte proliferation and liver regeneration and is involved in HGF-induced ERK signaling pathway. TEC also regulates FGF2 unconventional secretion (endoplasmic reticulum (ER)/Golgi-independent mechanism) under various physiological conditions through phosphorylation of FGF2 'Tyr-215'. May also be involved in the regulation of osteoclast differentiation.
See full target information TEC
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