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AB167855

Recombinant Human Tef protein (His tag N-Terminus)

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Recombinant Human Tef protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 303 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

KIAA1655, TEF, Thyrotroph embryonic factor

1 Images
SDS-PAGE - Recombinant Human Tef protein (His tag N-Terminus) (AB167855)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Tef protein (His tag N-Terminus) (AB167855)

15% SDS-PAGE analysis of ab167855 (3µg). Molecular size on SDS-PAGE appears higher.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q10587

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMSDAGGGKKPPVDPQAGPGPGPGRAAGERGLSGSFPLVLKKLMENPPREARLDKEKGKEKLEEDEAAAASTMAVSASLMPPIWDKTIPYDGESFHLEYMDLDEFLLENGIPASPTHLAHNLLLPVAELEGKESASSSTASPPSSSTAIFQPSETVSSTESSLEKERETPSPIDPNCVEVDVNFNPDPADLVLSSVPGGELFNPRKHKFAEEDLKPQPMIKKAKKVFVPDEQKDEKYWTRRKKNNVAAKRSRDARRLKENQITIRAAFLEKENTALRTEVAELRKEVGKCKTIVSKYETKYGPL","proteinLength":"Full Length","predictedMolecularWeight":"35.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":303,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q10587","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 'Tef' protein also known as thyrotroph embryonic factor plays a mechanistic role as a transcription factor. It weighs approximately 31 kilodaltons. Tef is expressed in several tissues but most prominently within the liver as well as in the heart. This protein belongs to the PAR bZip (proline and acidic amino acid-rich basic leucine zipper) family which is recognized for its ability to bind to DNA sequences to regulate transcriptional activity.
Biological function summary

The 'Tef' protein engages in important cellular processes that control circadian rhythms. It operates in a complex with other transcription factors which include DBP and HLF. These factors coordinate to regulate the expression of genes that follow a circadian pattern. The ability of Tef protein to influence gene expression at different times of the day allows it to maintain the internal biological clock's precision affecting metabolic and sleep-related activities.

Pathways

There is considerable involvement of 'Tef' in the regulation of the circadian rhythm pathway. Additionally Tef integrates with the glucose metabolism pathway influencing gene expression tied to metabolic cycles. Proteins such as CLOCK and BMAL1 are related through these pathways orchestrating gene networks critical to diurnal variations and energy homeostasis.

'Tef' is linked to metabolic syndrome and sleep disorders. Its influence on circadian rhythms makes Tef an important protein in diseases related to the disruption of these cycles. Anomalies in its pathways may connect with other proteins like PER1 and CRY which factor into the development of symptoms associated with metabolic imbalance and disordered sleep patterns.
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Specifications

Form

Liquid

Additional notes

ab167855 was purified using conventional chromatography techniques.

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General info

Function

Transcription factor that binds to and transactivates the TSHB promoter. Binds to a minimal DNA-binding sequence 5'-[TC][AG][AG]TTA[TC][AG]-3'.

Sequence similarities

Belongs to the bZIP family. PAR subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Transcription factor that binds to and transactivates the TSHB promoter. Binds to a minimal DNA-binding sequence 5'-[TC][AG][AG]TTA[TC][AG]-3'.
See full target information TEF
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