Recombinant Human TESK1 protein is a Human Full Length protein, in the 1 to 626 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, WB.
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Key facts
- Purity
- >70% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues (By similarity). Regulates the cellular cytoskeleton by enhancing actin stress fiber formation via phosphorylation of cofilin and by preventing microtubule breakdown via inhibition of TAOK1/MARKK kinase activity (By similarity). Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1 (By similarity). Positively regulates integrin-mediated cell spreading, via phosphorylation of cofilin (PubMed:15584898). Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of ciliary vesicle directional trafficking to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865). Probably plays a central role at and after the meiotic phase of spermatogenesis (By similarity).
Alternative names
Dual specificity testis-specific protein kinase 1, Testicular protein kinase 1, TESK1
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Recombinant Human TESK1 protein is a Human Full Length protein, in the 1 to 626 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >70% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- Q15569-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- Accession
- Q15569
- Protein length
- Full Length
- Predicted molecular weight
- 106 kDa
- Amino acids
- 1 to 626
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Purity was determined to be >70% by densitometry. Affinity purified.
General info
- Function
Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues (By similarity). Regulates the cellular cytoskeleton by enhancing actin stress fiber formation via phosphorylation of cofilin and by preventing microtubule breakdown via inhibition of TAOK1/MARKK kinase activity (By similarity). Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1 (By similarity). Positively regulates integrin-mediated cell spreading, via phosphorylation of cofilin (PubMed:15584898). Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of ciliary vesicle directional trafficking to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865). Probably plays a central role at and after the meiotic phase of spermatogenesis (By similarity).
- Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
- Post-translational modifications
Autophosphorylated on serine and tyrosine residues.
- Subcellular localisation
- Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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1 product image
SDS-PAGE - Recombinant Human TESK1 protein (ab125542)
SDS-PAGE analysis of ab125542.
Downloads
Product protocols
- Visit the general protocols
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