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AB99213

Recombinant Human Testin protein (His tag N-Terminus)

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Recombinant Human Testin protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 421 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Testin, TESS, TES

2 Images
SDS-PAGE - Recombinant Human Testin protein (AB99213)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Testin protein (AB99213)

SDS-PAGE analysis of ab99213

SDS-PAGE - Recombinant Human Testin protein (AB99213)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Testin protein (AB99213)

15% SDS-PAGE analysis of 3μg ab99213.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9UGI8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMDLENKVKKMGLGHEQGFGAPCLKCKEKCEGFELHFWRKICRNCKCGQEEHDVLLSNEEDRKVGKLFEDTKYTTLIAKLKSDGIPMYKRNVMILTNPVAAKKNVSINTVTYEWAPPVQNQALARQYMQMLPKEKQPVAGSEGAQYRKKQLAKQLPAHDQDPSKCHELSPREVKEMEQFVKKYKSEALGVGDVKLPCEMDAQGPKQMNIPGGDRSTPAAVGAMEDKSAEHKRTQYSCYCCKLSMKEGDPAIYAERAGYDKLWHPACFVCSTCHELLVDMIYFWKNEKLYCGRHYCDSEKPRCAGCDELIFSNEYTQAENQNWHLKHFCCFDCDSILAGEIYVMVNDKPVCKPCYVKNHAVVCQGCHNAIDPEVQRVTYNNFSWHASTECFLCSCCSKCLIGQKFMPVEGMVFCSVECKKRMS","proteinLength":"Full Length","predictedMolecularWeight":"50.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":421,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UGI8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Testin also known as TES is a protein with a mass of approximately 47 kDa. It functions as a scaffold protein involved in various cellular processes including cell adhesion and cytoskeletal organization. Testin predominantly localizes at cell-cell and cell-extracellular matrix adhesions providing structural stability and support. Expression of Testin occurs in numerous human tissues with higher levels in kidneys testes and pancreas suggesting its diverse role across the body.
Biological function summary

Testin plays a major role in regulating cell communication and morphology. It integrates into larger protein complexes that modulate focal adhesion dynamics and tissue architecture. By interacting with actin filaments and other cytoskeletal proteins Testin contributes to the maintenance of cell shape and motility. Its presence in complexes highlights its role in supporting cellular infrastructure and response to environmental signals.

Pathways

Testin participates notably in the integrin signaling and actin cytoskeleton rearrangement pathways. It acts as an important link between signaling molecules and structural components within cells. Interactions with proteins such as vinculin and paxillin lead to changes in cell adhesion and migration. These pathways are important because they connect cellular mechanics to responses influencing how cells move and bind in different contexts.

Testin is linked to cancer progression and neurodegenerative conditions. Altered expression of Testin has associations with tumor metastasis where it connects to proteins like FAK and Src which are known for driving cancer cell invasion. In neurodegenerative diseases Testin's role in maintaining cytoskeletal integrity and synaptic function suggests it might have protective or detrimental effects depending on its expression and interaction with proteins like tau or amyloid-beta.
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Specifications

Form

Liquid

Additional notes

ab99213 was purified using conventional chromatography techniques.

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General info

Function

Scaffold protein that may play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Plays a role in the regulation of cell proliferation. May act as a tumor suppressor. Inhibits tumor cell growth.

Sequence similarities

Belongs to the prickle / espinas / testin family.

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Product protocols

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Target data

Scaffold protein that may play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Plays a role in the regulation of cell proliferation. May act as a tumor suppressor. Inhibits tumor cell growth.
See full target information TES
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Complementary Products

Primary Antibodies

AB224375

Anti-Testin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Testin antibody (AB224375)

  • 0
  • 0
Assay Kits

AB113851

DCFDA / H2DCFDA - Cellular ROS Assay Kit

Cellular Activity - DCFDA / H2DCFDA - Cellular ROS Assay Kit (AB113851)

  • 5
  • 11
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB16502

Anti-NF-kB p65 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody (AB16502)

  • 4
  • 40

Product promise

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