Recombinant Human TET3 protein (Tagged) is a Human Fragment protein, in the 824 to 1795 aa range, expressed in Baculovirus infected Sf9, with >=50% purity and suitable for SDS-PAGE.
E F P T C D C V E Q I V E K D E G P Y Y T H L G S G P T V A S I R E L M E E R Y G E K G K A I R I E K V I Y T G K E G K S S R G C P I A K W V I R R H T L E E K L L C L V R H R A G H H C Q N A V I V I L I L A W E G I P R S L G D T L Y Q E L T D T L R K Y G N P T S R R C G L N D D R T C A C Q G K D P N T C G A S F S F G C S W S M Y F N G C K Y A R S K T P R K F R L A G D N P K E E E V L R K S F Q D L A T E V A P L Y K R L A P Q A Y Q N Q V T N E E I A I D C R L G L K E G R P F A G V T A C M D F C A H A H K D Q H N L Y N G C T V V C T L T K E D N R C V G K I P E D E Q L H V L P L Y K M A N T D E F G S E E N Q N A K V G S G A I Q V L T A F P R E V R R L P E P A K S C R Q R Q L E A R K A A A E K K K I Q K E K L S T P E K I K Q E A L E L A G I T S D P G L S L K G G L S Q Q G L K P S L K V E P Q N H F S S F K Y S G N A V V E S Y S V L G N C R P S D P Y S M N S V Y S Y H S Y Y A Q P S L T S V N G F H S K Y A L P S F S Y Y G F P S S N P V F P S Q F L G P G A W G H S G S S G S F E K K P D L H A L H N S L S P A Y G G A E F A E L P S Q A V P T D A H H P T P H H Q Q P A Y P G P K E Y L L P K A P L L H S V S R D P S P F A Q S S N C Y N R S I K Q E P V D P L T Q A E P V P R D A G K M G K T P L S E V S Q N G G P S H L W G Q Y S G G P S M S P K R T N G V G G S W G V F S S G E S P A I V P D K L S S F G A S C L A P S H F T D G Q W G L F P G E G Q Q A A S H S G G R L R G K P W S P C K F G N S T S A L A G P S L T E K P W A L G A G D F N S A L K G S P G F Q D K L W N P M K G E E G R I P A A G A S Q L D R A W Q S F G L P L G S S E K L F G A L K S E E K L W D P F S L E E G P A E E P P S K G A V K E E K G G G G A E E E E E E L W S D S E H N F L D E N I G G V A V A P A H G S I L I E C A R R E L H A T T P L K K P N R C H P T R I S L V F Y Q H K N L N Q P N H G L A L W E A K M K Q L A E R A R A R Q E E A A R L G L G Q Q E A K L Y G K K R K W G G T V V A E P Q Q K E K K G V V P T R Q A L A V P T D S A V T V S S Y A Y T
Application | Reactivity | Dilution info | Notes |
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Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization (PubMed:31928709). Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation (By similarity). Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes (PubMed:23217707). In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity). In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT (PubMed:23353889). Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG (PubMed:29276034).
KIAA0401, TET3, Methylcytosine dioxygenase TET3
Recombinant Human TET3 protein (Tagged) is a Human Fragment protein, in the 824 to 1795 aa range, expressed in Baculovirus infected Sf9, with >=50% purity and suitable for SDS-PAGE.
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride
Affinity purified.
Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization (PubMed:31928709). Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation (By similarity). Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes (PubMed:23217707). In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity). In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT (PubMed:23353889). Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG (PubMed:29276034).
Belongs to the TET family.
Monoubiquitinated at Lys-994 by the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex called CRL4(VprBP) or CUL4A-RBX1-DDB1-DCAF1/VPRBP complex; this modification promotes binding to DNA.
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SDS-PAGE analysis of ab271756.
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