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Recombinant Human TET3 protein (Tagged) is a Human Fragment protein, in the 824 to 1795 aa range, expressed in Baculovirus infected Sf9, with >=50% purity and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human TET3 protein (Tagged) (AB271756), expandable thumbnail

Key facts

Purity
>=50% SDS-PAGE
Expression system
Baculovirus infected Sf9 cells
Tags
DDDDK tag N-Terminus His tag C-Terminus
Applications
SDS-PAGE
Biologically active
No

Amino acid sequence

E F P T C D C V E Q I V E K D E G P Y Y T H L G S G P T V A S I R E L M E E R Y G E K G K A I R I E K V I Y T G K E G K S S R G C P I A K W V I R R H T L E E K L L C L V R H R A G H H C Q N A V I V I L I L A W E G I P R S L G D T L Y Q E L T D T L R K Y G N P T S R R C G L N D D R T C A C Q G K D P N T C G A S F S F G C S W S M Y F N G C K Y A R S K T P R K F R L A G D N P K E E E V L R K S F Q D L A T E V A P L Y K R L A P Q A Y Q N Q V T N E E I A I D C R L G L K E G R P F A G V T A C M D F C A H A H K D Q H N L Y N G C T V V C T L T K E D N R C V G K I P E D E Q L H V L P L Y K M A N T D E F G S E E N Q N A K V G S G A I Q V L T A F P R E V R R L P E P A K S C R Q R Q L E A R K A A A E K K K I Q K E K L S T P E K I K Q E A L E L A G I T S D P G L S L K G G L S Q Q G L K P S L K V E P Q N H F S S F K Y S G N A V V E S Y S V L G N C R P S D P Y S M N S V Y S Y H S Y Y A Q P S L T S V N G F H S K Y A L P S F S Y Y G F P S S N P V F P S Q F L G P G A W G H S G S S G S F E K K P D L H A L H N S L S P A Y G G A E F A E L P S Q A V P T D A H H P T P H H Q Q P A Y P G P K E Y L L P K A P L L H S V S R D P S P F A Q S S N C Y N R S I K Q E P V D P L T Q A E P V P R D A G K M G K T P L S E V S Q N G G P S H L W G Q Y S G G P S M S P K R T N G V G G S W G V F S S G E S P A I V P D K L S S F G A S C L A P S H F T D G Q W G L F P G E G Q Q A A S H S G G R L R G K P W S P C K F G N S T S A L A G P S L T E K P W A L G A G D F N S A L K G S P G F Q D K L W N P M K G E E G R I P A A G A S Q L D R A W Q S F G L P L G S S E K L F G A L K S E E K L W D P F S L E E G P A E E P P S K G A V K E E K G G G G A E E E E E E L W S D S E H N F L D E N I G G V A V A P A H G S I L I E C A R R E L H A T T P L K K P N R C H P T R I S L V F Y Q H K N L N Q P N H G L A L W E A K M K Q L A E R A R A R Q E E A A R L G L G Q Q E A K L Y G K K R K W G G T V V A E P Q Q K E K K G V V P T R Q A L A V P T D S A V T V S S Y A Y T

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization (PubMed:31928709). Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation (By similarity). Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes (PubMed:23217707). In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity). In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT (PubMed:23353889). Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG (PubMed:29276034).

Alternative names

Recommended products

Recombinant Human TET3 protein (Tagged) is a Human Fragment protein, in the 824 to 1795 aa range, expressed in Baculovirus infected Sf9, with >=50% purity and suitable for SDS-PAGE.

Key facts

Purity
>=50% SDS-PAGE
Expression system
Baculovirus infected Sf9 cells
Applications
SDS-PAGE
Accession
O43151-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

Sequence info

Amino acid sequence

E F P T C D C V E Q I V E K D E G P Y Y T H L G S G P T V A S I R E L M E E R Y G E K G K A I R I E K V I Y T G K E G K S S R G C P I A K W V I R R H T L E E K L L C L V R H R A G H H C Q N A V I V I L I L A W E G I P R S L G D T L Y Q E L T D T L R K Y G N P T S R R C G L N D D R T C A C Q G K D P N T C G A S F S F G C S W S M Y F N G C K Y A R S K T P R K F R L A G D N P K E E E V L R K S F Q D L A T E V A P L Y K R L A P Q A Y Q N Q V T N E E I A I D C R L G L K E G R P F A G V T A C M D F C A H A H K D Q H N L Y N G C T V V C T L T K E D N R C V G K I P E D E Q L H V L P L Y K M A N T D E F G S E E N Q N A K V G S G A I Q V L T A F P R E V R R L P E P A K S C R Q R Q L E A R K A A A E K K K I Q K E K L S T P E K I K Q E A L E L A G I T S D P G L S L K G G L S Q Q G L K P S L K V E P Q N H F S S F K Y S G N A V V E S Y S V L G N C R P S D P Y S M N S V Y S Y H S Y Y A Q P S L T S V N G F H S K Y A L P S F S Y Y G F P S S N P V F P S Q F L G P G A W G H S G S S G S F E K K P D L H A L H N S L S P A Y G G A E F A E L P S Q A V P T D A H H P T P H H Q Q P A Y P G P K E Y L L P K A P L L H S V S R D P S P F A Q S S N C Y N R S I K Q E P V D P L T Q A E P V P R D A G K M G K T P L S E V S Q N G G P S H L W G Q Y S G G P S M S P K R T N G V G G S W G V F S S G E S P A I V P D K L S S F G A S C L A P S H F T D G Q W G L F P G E G Q Q A A S H S G G R L R G K P W S P C K F G N S T S A L A G P S L T E K P W A L G A G D F N S A L K G S P G F Q D K L W N P M K G E E G R I P A A G A S Q L D R A W Q S F G L P L G S S E K L F G A L K S E E K L W D P F S L E E G P A E E P P S K G A V K E E K G G G G A E E E E E E L W S D S E H N F L D E N I G G V A V A P A H G S I L I E C A R R E L H A T T P L K K P N R C H P T R I S L V F Y Q H K N L N Q P N H G L A L W E A K M K Q L A E R A R A R Q E E A A R L G L G Q Q E A K L Y G K K R K W G G T V V A E P Q Q K E K K G V V P T R Q A L A V P T D S A V T V S S Y A Y T
Accession
O43151
Protein length
Fragment
Amino acids
824 to 1795
Nature
Recombinant
Tags
DDDDK tag N-Terminus, His tag C-Terminus

Specifications

Form
Liquid
Additional notes

Affinity purified.

General info

Function

Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization (PubMed:31928709). Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation (By similarity). Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes (PubMed:23217707). In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity). In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT (PubMed:23353889). Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG (PubMed:29276034).

Sequence similarities

Belongs to the TET family.

Post-translational modifications

Monoubiquitinated at Lys-994 by the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex called CRL4(VprBP) or CUL4A-RBX1-DDB1-DCAF1/VPRBP complex; this modification promotes binding to DNA.

Subcellular localisation
Nucleus

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle

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1 product image

  • SDS-PAGE - Recombinant Human TET3 protein (Tagged) (ab271756), expandable thumbnail

    SDS-PAGE - Recombinant Human TET3 protein (Tagged) (ab271756)

    SDS-PAGE analysis of ab271756.

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Product protocols

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