Recombinant Human TFB1M protein is a Human Full Length protein, in the 28 to 346 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M Q A A K Q L S Q N F L L D L R L T D K I V R K A G N L T N A Y V Y E V G P G P G G I T R S I L N A D V A E L L V V E K D T R F I P G L Q M L S D A A P G K L R I V H G D V L T F K V E K A F S E S L K R P W E D D P P N V H I I G N L P F S V S T P L I I K W L E N I S C R D G P F V Y G R T Q M T L T F Q K E V A E R L A A N T G S K Q R S R L S V M A Q Y L C N V R H I F T I P G Q A F V P K P E V D V G V V H F T P L I Q P K I E Q P F K L V E K V V Q N V F Q F R R K Y C H R G L R M L F P E A Q R L E S T G R L L E L A D I D P T L R P R Q L S I S H F K S L C D V Y R K M C D E D P Q L F A Y N F R E E L K R R K S K N E E K E E D D A E N Y R L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Mitochondrial methyltransferase which uses S-adenosyl methionine to dimethylate two highly conserved adjacent adenosine residues (A1583 and A1584) within the loop of helix 45 at the 3-prime end of 12S rRNA, thereby regulating the assembly or stability of the small subunit of the mitochondrial ribosome (PubMed:12496758, PubMed:25305075, PubMed:31251801). Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity (PubMed:11809803, PubMed:12068295, PubMed:12897151).
Alternative names
CGI-75, TFB1M, Mitochondrial 12S rRNA dimethylase 1, Mitochondrial transcription factor B1, h-mtTFB, h-mtTFB1, hTFB1M, mtTFB1
Recommended products
Recombinant Human TFB1M protein is a Human Full Length protein, in the 28 to 346 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- No
- Accession
- Q8WVM0-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M Q A A K Q L S Q N F L L D L R L T D K I V R K A G N L T N A Y V Y E V G P G P G G I T R S I L N A D V A E L L V V E K D T R F I P G L Q M L S D A A P G K L R I V H G D V L T F K V E K A F S E S L K R P W E D D P P N V H I I G N L P F S V S T P L I I K W L E N I S C R D G P F V Y G R T Q M T L T F Q K E V A E R L A A N T G S K Q R S R L S V M A Q Y L C N V R H I F T I P G Q A F V P K P E V D V G V V H F T P L I Q P K I E Q P F K L V E K V V Q N V F Q F R R K Y C H R G L R M L F P E A Q R L E S T G R L L E L A D I D P T L R P R Q L S I S H F K S L C D V Y R K M C D E D P Q L F A Y N F R E E L K R R K S K N E E K E E D D A E N Y R L
- Accession
- Q8WVM0
- Protein length
- Full Length
- Predicted molecular weight
- 38.8 kDa
- Amino acids
- 28 to 346
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab113141 was purified using conventional chromatography.
General info
- Function
Mitochondrial methyltransferase which uses S-adenosyl methionine to dimethylate two highly conserved adjacent adenosine residues (A1583 and A1584) within the loop of helix 45 at the 3-prime end of 12S rRNA, thereby regulating the assembly or stability of the small subunit of the mitochondrial ribosome (PubMed:12496758, PubMed:25305075, PubMed:31251801). Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity (PubMed:11809803, PubMed:12068295, PubMed:12897151).
- Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.
- Subcellular localisation
- Mitochondrion
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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1 product image
SDS-PAGE - Recombinant Human TFB1M protein (ab113141)
15% SDS-PAGE analysis of ab113141 (3μg)
Downloads
Product protocols
- Visit the general protocols
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