JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB134620

Recombinant Human TFB2M protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human TFB2M protein is a Human Full Length protein, in the 20 to 396 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

NS5ATP5, TFB2M, Hepatitis C virus NS5A-transactivated protein 5, Mitochondrial 12S rRNA dimethylase 2, Mitochondrial transcription factor B2, HCV NS5A-transactivated protein 5, h-mtTFB, h-mtTFB2, hTFB2M, mtTFB2

1 Images
SDS-PAGE - Recombinant Human TFB2M protein (AB134620)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human TFB2M protein (AB134620)

15% SDS-PAGE analysis of 3 μg ab134620.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9H5Q4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAGRFCILGSEAATRKHLPARNHCGLSDSSPQLWPEPDFRNPPRKASKASLDFKRYVTDRRLAETLAQIYLGKPSRPPHLLLECNPGPGILTQALLEAGAKVVALESDKTFIPHLESLGKNLDGKLRVIHCDFFKLDPRSGGVIKPPAMSSRGLFKNLGIEAVPWTADIPLKVVGMFPSRGEKRALWKLAYDLYSCTSIYKFGRIEVNMFIGEKEFQKLMADPGNPDLYHVLSVIWQLACEIKVLHMEPWSSFDIYTRKGPLENPKRRELLDQLQQKLYLIQMIPRQNLFTKNLTPMNYNIFFHLLKHCFGRRSATVIDHLRSLTPLDARDILMQIGKQEDEKVVNMHPQDFKTLFETIERSKDCAYKWLYDETLEDR","proteinLength":"Full Length","predictedMolecularWeight":"45.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":396,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9H5Q4","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column
style
left-column

Specifications

Form

Liquid

Additional notes

ab134620 is purified using conventional chromatography techniques.

style
left-column

General info

Function

S-adenosyl-L-methionine-dependent rRNA methyltransferase which may methylate two specific adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 12S mitochondrial rRNA (Probable). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed : 12068295, PubMed : 15526033, PubMed : 20410300, PubMed : 29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed : 15526033, PubMed : 29149603). Stimulates transcription independently of the methyltransferase activity (PubMed : 12897151).

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.

Subcellular localisation

Mitochondrion

style
left-column

Product protocols

style
left-column

Target data

S-adenosyl-L-methionine-dependent rRNA methyltransferase which may methylate two specific adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 12S mitochondrial rRNA (Probable). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed : 12068295, PubMed : 15526033, PubMed : 20410300, PubMed : 29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed : 15526033, PubMed : 29149603). Stimulates transcription independently of the methyltransferase activity (PubMed : 12897151).
See full target information TFB2M
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB103784

Recombinant Human mtTFA protein

SDS-PAGE - Recombinant Human mtTFA protein (AB103784)

  • 0
  • 0
Primary Antibodies

AB118321

Anti-TFB2M antibody

Western blot - Anti-TFB2M antibody (AB118321)

  • 0
  • 0
Primary Antibodies

AB185483

Anti-Secretory lectin ZG16 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Secretory lectin ZG16 antibody (AB185483)

  • 5
  • 1
Primary Antibodies

AB77616

Anti-Stra6 antibody

Western blot - Anti-Stra6 antibody (AB77616)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com