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AB204777

Recombinant Human TFIIE alpha/GTF2E1 protein (6x His tag C-Terminus)

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Recombinant Human TFIIE alpha/GTF2E1 protein (6x His tag C-Terminus) is a Human Full Length protein, in the 1 to 439 aa range, expressed in Escherichia coli, with >70%, suitable for SDS-PAGE, WB, Mass Spec.

View Alternative Names

TF2E1, GTF2E1, General transcription factor IIE subunit 1, General transcription factor IIE 56 kDa subunit, Transcription initiation factor IIE subunit alpha, TFIIE-alpha

2 Images
Western blot - Recombinant Human TFIIE alpha/GTF2E1 protein (His tag) (AB204777)
  • WB

Supplier Data

Western blot - Recombinant Human TFIIE alpha/GTF2E1 protein (His tag) (AB204777)

Western blot analysis of ab204777.

All lanes:

Western blot - Recombinant Human TFIIE alpha/GTF2E1 protein (His tag) (ab204777)

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SDS-PAGE - Recombinant Human TFIIE alpha/GTF2E1 protein (His tag) (AB204777)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TFIIE alpha/GTF2E1 protein (His tag) (AB204777)

10% SDS-PAGE analysis of ab204777 stained with Coomassie Blue.

Key facts

Purity

>70%

Expression system

Escherichia coli

Tags

6x His tag C-Terminus

Applications

Mass Spec, SDS-PAGE, WB

applications

Biologically active

No

Accession

P29083

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.3 Preservative: 1.7% Imidazole Constituents: 0.88% Sodium chloride, 0.36% Sodium phosphate

storage-buffer

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB248917

Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (AB248917)

  • 0
  • 0
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5
Secondary Antibodies

AB99695

Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as TFIIE alpha
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Sequence info

[{"linker":null,"sequence":"MADPDVLTEVPAALKRLAKYVIRGFYGIEHALALDILIRNSCVKEEDMLELLKFDRKQLRSVLNNLKGDKFIKCRMRVETAADGKTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERDSTNRASFKCPVCSSTFTDLEANQLFDPMTGTFRCTFCHTEVEEDESAMPKKDARTLLARFNEQIEPIYALLRETEDVNLAYEILEPEPTEIPALKQSKDHAATTAGAASLAGGHHREAWATKGPSYEDLYTQNVVINMDDQEDLHRASLEGKSAKERPIWLRESTVQGAYGSEDMKEGGIDMDAFQEREEGHAGPDDNEEVMRALLIHEKKTSSAMAGSVGAAAPVTAANGSDSESETSESDDDSPPRPAAVAVHKREEDEEEDDEFEEVADDPIVMVAGRPFSYSEVSQRPELVAQMTPEEKEAYIAMGQRMFEDLFE","proteinLength":"Full Length","predictedMolecularWeight":"51 kDa","actualMolecularWeight":null,"aminoAcidEnd":439,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P29083","tags":[{"tag":"6x His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab204777 was purified by Immobilized-metal affinity chromatography (IMAC) .

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General info

Function

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

Sequence similarities

Belongs to the TFIIE alpha subunit family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
See full target information GTF2E1
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Product promise

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