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AB81891

Recombinant Human TFIIF protein

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Recombinant Human TFIIF protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, GSA, EMSA.

View Alternative Names

RAP74, GTF2F1, General transcription factor IIF subunit 1, General transcription factor IIF 74 kDa subunit, Transcription initiation factor IIF subunit alpha, Transcription initiation factor RAP74, TFIIF-alpha

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

GSA, SDS-PAGE, EMSA

applications

Biologically active

No

Accession

P35269

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.9 Constituents: 20% Glycerol (glycerin, glycerine), 0.75% Potassium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00584% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "GSA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "EMSA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

1 unit equals 1 nanogram of purified protein.
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P35269","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

Recombinant RAP74 protein is isolated from an E. coli strain that carries the coding sequence of the human RAP74 subunit of TFIIF under the control of T7 promoter; recombinant RAP30 protein is isolated from an E. coli strain that carries the coding sequence of the human RAP30 subunit of TFIIF under the control of T7 promoter; TFIIF is reconstituted by combining equimolar quantities of recombinant RAP30 and RAP74.

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General info

Function

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.

Sequence similarities

Belongs to the TFIIF alpha subunit family.

Post-translational modifications

Phosphorylated on Ser and other residues by TAF1 and casein kinase II-like kinases.

Subcellular localisation

Nucleus

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Product protocols

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Target data

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.
See full target information GTF2F1
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Product promise

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