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AB283419

Recombinant human TGF alpha protein (Active)

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Recombinant human TGF alpha protein (Active) is a Human Full Length protein, in the 24 to 98 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, Biological Activity, HPLC, Cell Culture, FuncS.

View Alternative Names

Protransforming growth factor alpha, TGFA

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Biochemical assay - Recombinant human TGF alpha protein (Active) (AB283419)
  • Biochemical assay

Supplier Data

Biochemical assay - Recombinant human TGF alpha protein (Active) (AB283419)

Fully biologically active determined by the dose dependent proliferation of Balb/c-3T3 cells. ED50 <= 0.20 ng/ml, corresponding to a specific activity of 5x106 units/mg.

Mass Spectrometry - Recombinant human TGF alpha protein (Active) (AB283419)
  • Mass Spec

Unknown

Mass Spectrometry - Recombinant human TGF alpha protein (Active) (AB283419)

Mass determination by ESI-TOF.

Predicted MW is 8057 (+/- 10Da by ESI-TOF). MW observed is 8058.65.

SDS-PAGE - Recombinant human TGF alpha protein (Active) (AB283419)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human TGF alpha protein (Active) (AB283419)

SDS-PAGE analysis of ab283419

HPLC - Recombinant human TGF alpha protein (Active) (AB283419)
  • HPLC

Supplier Data

HPLC - Recombinant human TGF alpha protein (Active) (AB283419)

HPLC analysis of ab283419

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

FuncS, Biological Activity, HPLC, Cell Culture, SDS-PAGE, Mass Spec

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by the dose dependent proliferationof Balb/c-3T3 cells. ED50 is ≤ 0.20 ng/ml, corresponding to a specific activity of 5 x 106 units/mg.

Accession

P01135

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Biological Activity": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }
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Sequence info

[{"sequence":"ENSTSPLSADPPVAAAVVSHFNDCPDSHTQFCFHGTCRFLVQEDKPACVCHSGYVGARCEHADLLAVVAASQKKQ","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":98,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P01135","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

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General info

Function

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

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Product protocols

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Target data

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
See full target information Protransforming growth factor alpha
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Product promise

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