Recombinant human TGF alpha protein (Active) is a Human Full Length protein, in the 24 to 98 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, Biological Activity, HPLC, Cell Culture.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, MS, Biological Activity, HPLC, Cell Culture
- Biologically active
- Yes
Amino acid sequence
E N S T S P L S A D P P V A A A V V S H F N D C P D S H T Q F C F H G T C R F L V Q E D K P A C V C H S G Y V G A R C E H A D L L A V V A A S Q K K Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application Biological Activity | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Alternative names
Protransforming growth factor alpha, TGFA
Recommended products
Recombinant human TGF alpha protein (Active) is a Human Full Length protein, in the 24 to 98 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, Biological Activity, HPLC, Cell Culture.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, MS, Biological Activity, HPLC, Cell Culture
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependent proliferationof Balb/c-3T3 cells. ED50 is ≤ 0.20 ng/ml, corresponding to a specific activity of 5 x 106 units/mg.
- Accession
- P01135-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- E N S T S P L S A D P P V A A A V V S H F N D C P D S H T Q F C F H G T C R F L V Q E D K P A C V C H S G Y V G A R C E H A D L L A V V A A S Q K K Q
- Accession
- P01135
- Protein length
- Full Length
- Predicted molecular weight
- 17 kDa
- Amino acids
- 24 to 98
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
SDS-PAGE - Recombinant human TGF alpha protein (Active) (ab283419)
SDS-PAGE analysis of ab283419
Mass Spectrometry - Recombinant human TGF alpha protein (Active) (ab283419)
Mass determination by ESI-TOF.
Predicted MW is 8057 (+/- 10Da by ESI-TOF). MW observed is 8058.65.
HPLC - Recombinant human TGF alpha protein (Active) (ab283419)
HPLC analysis of ab283419
Biochemical assay - Recombinant human TGF alpha protein (Active) (ab283419)
Fully biologically active determined by the dose dependent proliferation of Balb/c-3T3 cells. ED50 <= 0.20 ng/ml, corresponding to a specific activity of 5x106 units/mg.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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