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Recombinant human TGF beta 1 protein (Active) is a Human Full Length protein, in the 279 to 390 aa range, expressed in CHO, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.


Publications

Key facts

Purity
>98% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
CHO cells
Tags
Tag free
Applications
SDS-PAGE, FuncS, HPLC
Biologically active
Yes

Amino acid sequence

A L D T N Y C F S S T E K N C C V R Q L Y I D F R K D L G W K W I H E P K G Y H A N F C L G P C P Y I W S L D T Q Y S K V L A L Y N Q H N P G A S A A P C C V P Q A L E P L P I V Y Y V G R K P K V E Q L S N M I V R S C K C S S S A L D T N Y C F S S T E K N C C V R Q L Y I D F R K D L G W K W I H E P K G Y H A N F C L G P C P Y I W S L D T Q Y S K V L A L Y N Q H N P G A S A A P C C V P Q A L E P L P I V Y Y V G R K P K V E Q L S N M I V R S C K C S

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

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Target data

Function

Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively. Latency-associated peptide. Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix (PubMed:28117447). Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1 (PubMed:19651619, PubMed:19750484, PubMed:2022183, PubMed:22278742, PubMed:8617200, PubMed:8939931). Interaction with LRRC33/NRROS regulates activation of TGF-beta-1 in macrophages and microglia (Probable). Interaction with LRRC32/GARP controls activation of TGF-beta-1 on the surface of activated regulatory T-cells (Tregs) (PubMed:19651619, PubMed:19750484, PubMed:22278742). Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1 (PubMed:22278742, PubMed:28117447). Transforming growth factor beta-1. Multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration (By similarity). Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains remain non-covalently linked rendering TGF-beta-1 inactive during storage in extracellular matrix (PubMed:29109152). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS that control activation of TGF-beta-1 and maintain it in a latent state during storage in extracellular milieus (PubMed:19651619, PubMed:19750484, PubMed:2022183, PubMed:22278742, PubMed:8617200, PubMed:8939931). TGF-beta-1 is released from LAP by integrins (ITGAV:ITGB6 or ITGAV:ITGB8): integrin-binding to LAP stabilizes an alternative conformation of the LAP bowtie tail and results in distortion of the LAP chain and subsequent release of the active TGF-beta-1 (PubMed:22278742, PubMed:28117447). Once activated following release of LAP, TGF-beta-1 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (PubMed:20207738). While expressed by many cells types, TGF-beta-1 only has a very localized range of action within cell environment thanks to fine regulation of its activation by Latency-associated peptide chain (LAP) and 'milieu molecules' (By similarity). Plays an important role in bone remodeling: acts as a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (By similarity). Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner (By similarity). At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development (By similarity). At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (By similarity). Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP) (PubMed:25310401). Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus (PubMed:25893292, PubMed:29483653, PubMed:30696809). Positively regulates odontoblastic differentiation in dental papilla cells, via promotion of IPO7-mediated translocation of phosphorylated SMAD2 to the nucleus and subsequent transcription of target genes (By similarity). Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (PubMed:25893292, PubMed:30696809).

Alternative names

Recommended products

Recombinant human TGF beta 1 protein (Active) is a Human Full Length protein, in the 279 to 390 aa range, expressed in CHO, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.

Key facts

Purity
>98% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
CHO cells
Applications
SDS-PAGE, FuncS, HPLC
Biological activity
The ED50, as determined by TGF beta 1's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells, is ≤ 0.05 ng/ml, corresponding to a specific activity of >2 x 107 units/mg
Accession
P01137-1
Animal free
No
Species
Human
Reconstitution
Centrifuge the vial prior to opening. For lot GR18648 Reconstitute in 10mM Citric Acid, pH 3.0 to 0.1-1.0 mg/ml. For all other lots reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. If you have any questions regaring the reconstitution of ab50038 please contact scientific support.
Concentration
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Sequence info

Amino acid sequence

A L D T N Y C F S S T E K N C C V R Q L Y I D F R K D L G W K W I H E P K G Y H A N F C L G P C P Y I W S L D T Q Y S K V L A L Y N Q H N P G A S A A P C C V P Q A L E P L P I V Y Y V G R K P K V E Q L S N M I V R S C K C S S S A L D T N Y C F S S T E K N C C V R Q L Y I D F R K D L G W K W I H E P K G Y H A N F C L G P C P Y I W S L D T Q Y S K V L A L Y N Q H N P G A S A A P C C V P Q A L E P L P I V Y Y V G R K P K V E Q L S N M I V R S C K C S
Accession
P01137
Protein length
Full Length
Predicted molecular weight
25 kDa
Amino acids
279 to 390
Nature
Recombinant

Specifications

Form
Lyophilized
Additional notes

>98% HPLC analyses.

General info

Function

Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively.

Sequence similarities

Belongs to the TGF-beta family.

Post-translational modifications

Transforming growth factor beta-1 proprotein: The precursor proprotein is cleaved in the Golgi apparatus by FURIN to form Transforming growth factor beta-1 (TGF-beta-1) and Latency-associated peptide (LAP) chains, which remain non-covalently linked, rendering TGF-beta-1 inactive.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA), Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.

This product is an active protein and may elicit a biological response in vivo, handle with caution.

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