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AB277760

Recombinant human TGF beta 2 protein (Active)

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(1 Publication)

Recombinant human TGF beta 2 protein (Active) is a Human Full Length protein, in the 21 to 414 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.

View Alternative Names

Transforming growth factor beta-2 proprotein, Cetermin, Glioblastoma-derived T-cell suppressor factor, G-TSF, TGFB2

4 Images
Mass Spectrometry - Recombinant human TGF beta 2 protein (Active) (AB277760)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human TGF beta 2 protein (Active) (AB277760)

Mass determination by ESI TOF.

Predicted mass is 45592.13 Da (+/- 1-Da by ESI TOF). Multiple peaks are due to proteolytic cleavage during expression. Peak1 (12720 Da, aa303-414); Peak 2 (32170 DA,aa 21-297; Peak 3 (45592 Da, aa 21-414).

Functional Studies - Recombinant human TGF beta 2 protein (Active) (AB277760)
  • FuncS

Supplier Data

Functional Studies - Recombinant human TGF beta 2 protein (Active) (AB277760)

Fully biologically active determoned by the dose-dependent inhibition of IL-4 dependent HT-2 cell proliferation.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3373524-1

SDS-PAGE - Recombinant human TGF beta 2 protein (Active) (AB277760)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human TGF beta 2 protein (Active) (AB277760)

SDS-PAGE analysis of ab277760.

HPLC - Recombinant human TGF beta 2 protein (Active) (AB277760)
  • HPLC

Supplier Data

HPLC - Recombinant human TGF beta 2 protein (Active) (AB277760)

HPLC analysis of ab277760.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, HPLC, FuncS, SDS-PAGE, Cell Culture

applications

Biologically active

Yes

Biological activity

Fully biologically active determoned by the dose-dependent inhibition of IL-4 dependent HT-2 cell proliferation.

Accession

P61812

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"LSTCSTLDMDQFMRKRIEAIRGQILSKLKLTSPPEDYPEPEEVPPEVISIYNSTRDLLQEKASRRAAACERERSDEEYYAKEVYKIDMPPFFPSENAIPPTFYRPYFRIVRFDVSAMEKNASNLVKAEFRVFRLQNPKARVPEQRIELYQILKSKDLTSPTQRYIDSKVVKTRAEGEWLSFDVTDAVHEWLHHKDRNLGFKISLHCPCCTFVPSNNYIIPNKSEELEARFAGIDGTSTYTSGDQKTIKSTRKKNSGKTPHLLLMLLPSYRLESQQTNRRKKRALDAAYCFRNVQDNCCLRPLYIDFKRDLGWKWIHEPKGYNANFCAGACPYLWSSDTQHSRVLSLYNTINPEASASPCCVSQDLEPLTILYYIGKTPKIEQLSNMIVKSCKCS","proteinLength":"Full Length","predictedMolecularWeight":"45.59 kDa","actualMolecularWeight":"45.59 kDa","aminoAcidEnd":414,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P61812","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

Additional notes

=95%  by HPLC

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General info

Function

Transforming growth factor beta-2 proprotein. Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.. Latency-associated peptide. Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix (By similarity). Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 (By similarity).. Transforming growth factor beta-2. Multifunctional protein that regulates various processes such as angiogenesis and heart development (PubMed : 22772368, PubMed : 22772371). Activation into mature form follows different steps : following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix (By similarity). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus (By similarity). Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (By similarity).

Sequence similarities

Belongs to the TGF-beta family.

Post-translational modifications

Transforming growth factor beta-2 proprotein. The precursor proprotein is cleaved in the Golgi apparatus to form Transforming growth factor beta-2 (TGF-beta-2) and Latency-associated peptide (LAP) chains, which remain non-covalently linked, rendering TGF-beta-2 inactive.

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Product protocols

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Target data

Transforming growth factor beta-2 proprotein. Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.. Latency-associated peptide. Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix (By similarity). Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 (By similarity).. Transforming growth factor beta-2. Multifunctional protein that regulates various processes such as angiogenesis and heart development (PubMed : 22772368, PubMed : 22772371). Activation into mature form follows different steps : following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix (By similarity). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus (By similarity). Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (By similarity).
See full target information TGFB2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 12:4374-4384 PubMed34304709

2021

Krüppel-like factor 1 (KLF1) promoted the proliferation, migration and invasion of human lens epithelial cells by enhancing the expression of Zinc Finger and BTB Domain Containing 7A (ZBTB7A) and activating Wnt/β-catenin pathway.

Applications

FuncS

Species

Unspecified reactive species

Guangming Shi,Feng Yang
View all publications
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