Recombinant Human TGF beta 3 protein (denatured) is a Human Full Length protein, in the 24 to 412 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S L S T C T T L D F G H I K K K R V E A I R G Q I L S K L R L T S P P E P T V M T H V P Y Q V L A L Y N S T R E L L E E M H G E R E E G C T Q E N T E S E Y Y A K E I H K F D M I Q G L A E H N E L A V C P K G I T S K V F R F N V S S V E K N R T N L F R A E F R V L R V P N P S S K R N E Q R I E L F Q I L R P D E H I A K Q R Y I G G K N L P T R G T A E W L S F D V T D T V R E W L L R R E S N L G L E I S I H C P C H T F Q P N G D I L E N I H E V M E I K F K G V D N E D D H G R G D L G R L K K Q K D H H N P H L I L M M I P P H R L D N P G Q G G Q R K K R A L D T N Y C F R N L E E N C C V R P L Y I D F R Q D L G W K W V H E P K G Y Y A N F C S G P C P Y L R S A D T T H S T V L G L Y N T L N P E A S A S P C C V P Q D L E P L T I L Y Y V G R T P K V E Q L S N M V V K S C K C S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transforming growth factor beta-3 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains, which constitute the regulatory and active subunit of TGF-beta-3, respectively.Latency-associated peptideRequired to maintain the Transforming growth factor beta-3 (TGF-beta-3) chain in a latent state during storage in extracellular matrix (By similarity). Associates non-covalently with TGF-beta-3 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-3 (By similarity). Interaction with integrins results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-3 (By similarity).Transforming growth factor beta-3: Multifunctional protein that regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development (By similarity). Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains remain non-covalently linked rendering TGF-beta-3 inactive during storage in extracellular matrix (By similarity). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP that control activation of TGF-beta-3 and maintain it in a latent state during storage in extracellular milieus (By similarity). TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3 (By similarity). Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (By similarity).
Alternative names
Transforming growth factor beta-3 proprotein, TGFB3
Recommended products
Recombinant Human TGF beta 3 protein (denatured) is a Human Full Length protein, in the 24 to 412 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P10600-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl
Sequence info
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S L S T C T T L D F G H I K K K R V E A I R G Q I L S K L R L T S P P E P T V M T H V P Y Q V L A L Y N S T R E L L E E M H G E R E E G C T Q E N T E S E Y Y A K E I H K F D M I Q G L A E H N E L A V C P K G I T S K V F R F N V S S V E K N R T N L F R A E F R V L R V P N P S S K R N E Q R I E L F Q I L R P D E H I A K Q R Y I G G K N L P T R G T A E W L S F D V T D T V R E W L L R R E S N L G L E I S I H C P C H T F Q P N G D I L E N I H E V M E I K F K G V D N E D D H G R G D L G R L K K Q K D H H N P H L I L M M I P P H R L D N P G Q G G Q R K K R A L D T N Y C F R N L E E N C C V R P L Y I D F R Q D L G W K W V H E P K G Y Y A N F C S G P C P Y L R S A D T T H S T V L G L Y N T L N P E A S A S P C C V P Q D L E P L T I L Y Y V G R T P K V E Q L S N M V V K S C K C S
- Accession
- P10600
- Protein length
Full Length
- Predicted molecular weight
47.2 kDa
- Amino acids
24 to 412
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
ab202611 was purified by using conventional chromatography techniques.
General info
- Function
Transforming growth factor beta-3 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains, which constitute the regulatory and active subunit of TGF-beta-3, respectively.
- Sequence similarities
Belongs to the TGF-beta family.
- Post-translational modifications
Transforming growth factor beta-3 proprotein: The precursor proprotein is cleaved in the Golgi apparatus to form Transforming growth factor beta-3 (TGF-beta-3) and Latency-associated peptide (LAP) chains, which remain non-covalently linked, rendering TGF-beta-3 inactive.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human TGF beta 3 protein (denatured) (ab202611)
15% SDS-PAGE analysis of ab202611 (3μg).
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