Recombinant Human TGT protein
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Recombinant Human TGT protein is a Human Full Length protein, in the 1 to 403 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
TGT, TGUT, QTRT1, Queuine tRNA-ribosyltransferase catalytic subunit 1, Guanine insertion enzyme, tRNA-guanine transglycosylase
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human TGT protein (AB202188)
15% SDS-PAGE analysis of ab202188 (3 μg).
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The TGT catalyzed guanine exchange on tRNA enhances the correct folding of tRNA and supports the accuracy of protein translation. The protein is a part of an RNA-modifying enzyme family and does not typically form complexes but acts independently on its tRNA substrates. Its activity ensures proper tRNA structure influencing protein synthesis efficiency and overall cellular protein homeostasis.
Pathways
TGT plays an important role in the tRNA modification pathway which is important for post-transcriptional RNA processing. It connects with pathways involved in protein synthesis and RNA stability. TGT's function is integrally linked with enzymes like tRNA synthetases which are responsible for charging tRNAs with their corresponding amino acids establishing a pivotal link in the genetic code translation process.
Specifications
Form
Liquid
Additional notes
ab202188 was purified using conventional chromatography techniques.
General info
Function
Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed : 11255023, PubMed : 20354154, PubMed : 34009357, PubMed : 34241577). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity). Modification of cytoplasmic tRNAs with queuosine controls the elongation speed of cognate codons, thereby ensuring the correct folding of nascent proteins to maintain proteome integrity (PubMed : 30093495).
Sequence similarities
Belongs to the queuine tRNA-ribosyltransferase family.
Subcellular localisation
Mitochondrion outer membrane
Target data
Complementary Products
Product promise
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