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AB124553

Recombinant Human THAP1 protein (denatured) (His tag N-Terminus)

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Recombinant Human THAP1 protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 213 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

THAP domain-containing protein 1, THAP1

1 Images
SDS-PAGE - Recombinant Human THAP1 protein (denatured) (His tag N-Terminus) (AB124553)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human THAP1 protein (denatured) (His tag N-Terminus) (AB124553)

15% SDS-PAGE (3 μg loaded)

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9NVV9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 12.01% Urea, 10% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMVQSCSAYGCKNRYDKDKPVSFHKFPLTRPSLCKEWEAAVRRKNFKPTKYSSICSEHFTPDCFKRECNNKLLKENAVPTIFLCTEPHDKKEDLLEPQEQLPPPPLPPPVSQVDAAIGLLMPPLQTPVNLSVFCDHNYTVEDTMHQRKRIHQLEQQVEKLRKKLKTAQQRCRRQERQLEKLKEVVHFQKEKDDVSERGYVILPNDYFEIVEVPA","proteinLength":"Full Length","predictedMolecularWeight":"27.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":213,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NVV9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

THAP1 also known as Thanatos-associated protein domain-containing apoptosis-associated protein 1 is a DNA-binding protein characterized by its THAP domain. It has a molecular mass of approximately 27 kDa. THAP1 is expressed widely in human tissues with significant presence in neurons and cardiac muscle. Mechanically it acts as a transcription factor regulating gene expression by binding to specific DNA sequences.
Biological function summary

THAP1 functions as an important regulator of gene transcription and is a part of various transcription complexes. It plays a role in cell proliferation apoptosis and the response to stress signals. This protein controls the expression of several genes involved in key cellular functions making it essential for normal cell cycle control and differentiation.

Pathways

THAP1 is involved in the regulation of the cell cycle and apoptosis pathways. It interacts with components of the retinoblastoma protein pathway to influence cell proliferation. THAP1 also has a regulatory relationship with E2F target genes which are important for controlling the progression of cells through the cell cycle.

Mutations in THAP1 associate with DYT6 dystonia a neurological movement disorder. The protein's dysfunction affects downstream targets in the dopamine metabolism pathway highlighting its role in maintaining normal dopaminergic function. Beyond this variations in THAP1 impact pulmonary hypertension by interacting with the BMPR2 signaling pathway indicating the protein's importance in vascular endothelial function.
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Specifications

Form

Liquid

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General info

Function

DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modulating expression of pRB-E2F cell-cycle target genes, including RRM1. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. May also have pro-apoptotic activity by potentiating both serum-withdrawal and TNF-induced apoptosis.

Sequence similarities

Belongs to the THAP1 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modulating expression of pRB-E2F cell-cycle target genes, including RRM1. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. May also have pro-apoptotic activity by potentiating both serum-withdrawal and TNF-induced apoptosis.
See full target information THAP1
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