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AB101158

Recombinant Human THEM2 protein

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Recombinant Human THEM2 protein is a Human Full Length protein, in the 1 to 140 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

THEM2, HT012, PNAS-27, ACOT13, Acyl-coenzyme A thioesterase 13, Acyl-CoA thioesterase 13, Hotdog-fold thioesterase superfamily member 2, Palmitoyl-CoA hydrolase, Thioesterase superfamily member 2

1 Images
SDS-PAGE - Recombinant Human THEM2 protein (AB101158)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human THEM2 protein (AB101158)

15% SDS-PAGE showing ab101158 at approximately 17.1kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9NPJ3

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMTSMTQSLREVIKAMTKARNFERVLGKITLVSAAPGKVICEMKVEEEHTNAIGTLHGGLTATLVDNISTMALLCTERGAPGVSVDMNITYMSPAKLGEDIVITAHVLKQGKTLAFTSVDLTNKATGKLIAQGRHTKHLGN","proteinLength":"Full Length","predictedMolecularWeight":"17.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":140,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NPJ3","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

THEM2 also known as thioesterase superfamily member 2 is a protein that belongs to the hotdog-fold enzyme superfamily. It acts as a thioesterase specifically hydrolyzing acyl-CoA thioesters. THEM2 has a molecular mass of approximately 15 kDa and can be found mainly expressed in skeletal muscle liver and adipose tissues. These tissues are key for metabolic activities indicating THEM2 plays a significant role in metabolism.
Biological function summary

THEM2 influences lipid metabolism and energy homeostasis. It does not function alone but operates as part of a larger enzyme complex. In particular THEM2 regulates lipid metabolic processes by controlling the levels of acyl-CoA substrates. Through these mechanisms THEM2 contributes to maintaining metabolic balance within the cells impacting both the storage and usage of energy resources.

Pathways

THEM2 participates in key metabolic pathways related to lipid metabolism. It is closely linked with the fatty acid metabolism pathway and impacts the regulation of fatty acid synthesis and degradation. THEM2 interacts with other proteins involved in these pathways such as Acyl-CoA Synthetase to maintain lipid homeostasis. This coordination ensures proper metabolic control integrating energy supply and demand.

THEM2 has correlations with metabolic disorders like obesity and type 2 diabetes. Abnormal THEM2 function can lead to imbalances in lipid metabolism contributing to the development of these conditions. It also shows connections with adiponectin a protein hormone involved in glucose regulation and fatty acid breakdown which may influence the progression of these disorders. Understanding THEM2 and its interactions helps identify potential therapeutic targets for treating metabolic diseases.
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Specifications

Form

Liquid

Additional notes

ab101158 is purified using conventional chromatography techniques.

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General info

Function

Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed : 16934754, PubMed : 19170545). Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates (By similarity) (PubMed : 16934754, PubMed : 19170545). Can also hydrolyze 3-hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro) (By similarity) (PubMed : 16934754, PubMed : 19170545). May play a role in controlling adaptive thermogenesis (By similarity).

Sequence similarities

Belongs to the thioesterase PaaI family.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed : 16934754, PubMed : 19170545). Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates (By similarity) (PubMed : 16934754, PubMed : 19170545). Can also hydrolyze 3-hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro) (By similarity) (PubMed : 16934754, PubMed : 19170545). May play a role in controlling adaptive thermogenesis (By similarity).
See full target information ACOT13
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Complementary Products

Primary Antibodies

AB254679

Anti-THEM2 antibody

Western blot - Anti-THEM2 antibody (AB254679)

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  • 0
Cell Lines & Lysates

AB301834

Human ACOT13 knockout K562 cell line

Advanced Validation

Western blot - Human ACOT13 knockout K562 cell line (AB301834)

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  • 0

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