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AB131686

Recombinant Human THG1L protein

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Recombinant Human THG1L protein is a Human Fragment protein, in the 30 to 298 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

ICF45, THG1L, Probable tRNA(His) guanylyltransferase, Induced in high glucose-1, Interphase cytoplasmic foci protein 45, tRNA-histidine guanylyltransferase, IHG-1

1 Images
SDS-PAGE - Recombinant Human THG1L protein (AB131686)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human THG1L protein (AB131686)

15% SDS PAGE analysis of 3 μg of ab131686.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9NWX6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAKSKFEYVRDFEADDTCLAHCWVVVRLDGRNFHRFAEKHNFAKPNDSRALQLMTKCAQTVMEELEDIVIAYGQSDEYSFVFKRKTNWFKRRASKFMTHVASQFASSYVFYWRDYFEDQPLLYPPGFDGRVVVYPSNQTLKDYLSWRQADCHINNLYNTVFWALIQQSGLTPVQAQGRLQGTLAADKNEILFSEFNINYNNELPMYRKGTVLIWQKVDEVMTKEIKLPTEMEGKKMAVTRTRTKPVPLHCDIIGDAFWKEHPEILDEDS","proteinLength":"Fragment","predictedMolecularWeight":"34 kDa","actualMolecularWeight":null,"aminoAcidEnd":298,"aminoAcidStart":30,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NWX6","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

THG1L also known as tRNA-histidine guanylyltransferase 1-like is an enzyme responsible for adding a guanine nucleotide to the 5' end of tRNA(His). This enzyme weighs approximately 48 kDa and is encoded by the THG1L gene. THG1L is highly conserved across eukaryotes and its expression is mostly observed in the mitochondria and cytosol of human cells. The protein ensures the proper modification and functioning of tRNA which is important for accurate protein synthesis.
Biological function summary

TRNA processing involves an important step facilitated by THG1L. This enzyme is important for maintaining the integrity of tRNA(His) by forming a specific guanine nucleotide cap at its 5' end. THG1L does not belong to any known large protein complex but works in coordination with other enzymes in the tRNA maturation process. It ensures the appropriate modifications in tRNAs which are essential for their correct recognition by ribosomes during translation.

Pathways

THG1L plays an essential role in the tRNA modification pathway particularly in histidine tRNA processing. The presence of THG1L facilitates the correct attachment of histidine to its tRNA therefore maintaining the fidelity of the translation process. This enzyme interacts with other proteins involved in the tRNA aminoacylation pathway including aminoacyl-tRNA synthetases which are important for the attachment of amino acids to their corresponding tRNAs during protein synthesis.

Researchers have investigated THG1L in the context of mitochondrial diseases and neurodegenerative disorders. Abnormalities in THG1L function can lead to improper tRNA processing which may result in mitochondrial dysfunction or contribute to the pathogenesis of neurological disorders. The interaction of THG1L with mitochondrial proteins such as those involved in oxidative phosphorylation suggests a potential link between mutations in THG1L and mitochondrial-related diseases.
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Specifications

Form

Liquid

Additional notes

ab131686 was purified using conventional chromatography techniques.

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General info

Function

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis (Probable). Also functions as a guanyl-nucleotide exchange factor/GEF for the MFN1 and MFN2 mitofusins thereby regulating mitochondrial fusion (PubMed : 25008184, PubMed : 27307223). By regulating both mitochondrial dynamics and bioenergetic function, it contributes to cell survival following oxidative stress (PubMed : 25008184, PubMed : 27307223).

Sequence similarities

Belongs to the tRNA(His) guanylyltransferase family.

Subcellular localisation

Mitochondrion outer membrane

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Product protocols

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Target data

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis (Probable). Also functions as a guanyl-nucleotide exchange factor/GEF for the MFN1 and MFN2 mitofusins thereby regulating mitochondrial fusion (PubMed : 25008184, PubMed : 27307223). By regulating both mitochondrial dynamics and bioenergetic function, it contributes to cell survival following oxidative stress (PubMed : 25008184, PubMed : 27307223).
See full target information THG1L
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