Recombinant Human THOC7 protein is a Human Full Length protein, in the 1 to 204 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M G A V T D D E V I R K R L L I D G D G A G D D R R I N L L V K S F I K W C N S G S Q E E G Y S Q Y Q R M L S T L S Q C E F S M G K T L L V Y D M N L R E M E N Y E K I Y K E I E C S I A G A H E K I A E C K K Q I L Q A K R I R K N R Q E Y D A L A K V I Q H H P D R H E T L K E L E A L G K E L E H L S H I K E S V E D K L E L R R K Q F H V L L S T I H E L Q Q T L E N D E K L S E V E E A Q E A S M E T D P K P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed:15833825, PubMed:15998806, PubMed:17190602). Required for efficient export of polyadenylated RNA (PubMed:23222130). Plays a key structural role in the oligomerization of the THO-DDX39B complex (PubMed:33191911). TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway (PubMed:15833825, PubMed:15998806, PubMed:17190602). (Microbial infection) The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.
Alternative names
NIF3L1BP1, THOC7, THO complex subunit 7, Functional spliceosome-associated protein 24, Ngg1-interacting factor 3-like protein 1-binding protein 1, hTREX30, fSAP24, NIF3L1-binding protein 1
Recombinant Human THOC7 protein is a Human Full Length protein, in the 1 to 204 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q6I9Y2-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M G A V T D D E V I R K R L L I D G D G A G D D R R I N L L V K S F I K W C N S G S Q E E G Y S Q Y Q R M L S T L S Q C E F S M G K T L L V Y D M N L R E M E N Y E K I Y K E I E C S I A G A H E K I A E C K K Q I L Q A K R I R K N R Q E Y D A L A K V I Q H H P D R H E T L K E L E A L G K E L E H L S H I K E S V E D K L E L R R K Q F H V L L S T I H E L Q Q T L E N D E K L S E V E E A Q E A S M E T D P K P
- Accession
- Q6I9Y2
- Protein length
- Full Length
- Predicted molecular weight
- 26.1 kDa
- Amino acids
- 1 to 204
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab202166 was purified using conventional chromatography techniques.
General info
- Function
Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed:15833825, PubMed:15998806, PubMed:17190602). Required for efficient export of polyadenylated RNA (PubMed:23222130). Plays a key structural role in the oligomerization of the THO-DDX39B complex (PubMed:33191911). TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway (PubMed:15833825, PubMed:15998806, PubMed:17190602).
- Sequence similarities
Belongs to the THOC7 family.
- Subcellular localisation
- Nucleus, Nucleus speckle
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
THOC7 also known as Transcription Export Protein 7 or HPR1 homolog is a protein involved in the mRNA export process from the nucleus to the cytoplasm. This protein weighs approximately 16 kDa. THOC7 is typically expressed in various human tissues including the heart kidney and skeletal muscle. The protein forms part of a multicomponent protein complex called the THO complex which integrates into the TRanscription-Export (TREX) complex.
- Biological function summary
THOC7 plays an important role in the stabilization and export of mRNA. Within the THO complex it contributes to maintaining RNA stability and is essential for efficient transcription elongation. It interacts closely with other components of the complex which ensures proper genomic expression regulation. During RNA processing THOC7's participation in the TREX complex is critical for attaching export-ready mRNA transcripts to the nuclear pore complex helping facilitate their export into the cytoplasm.
- Pathways
THOC7's involvement in the mRNA export pathway is indispensable for maintaining transcription homeostasis. It is essential for the coupling of transcription and mRNA processing interacting with proteins like THOC5 and those in the Aly/REF export factor family. THOC7 also engages in the cellular process of mRNA surveillance ensuring that only correctly processed mRNA molecules reach the cytoplasm for translation.
- Associated diseases and disorders
THOC7's malfunction can lead to disruptions in mRNA processing which may associate with cancer and neurodegenerative diseases. Defects or mutations in THOC7 or its associated TREX complex components have been observed in certain cancer types contributing to the abnormal regulation of gene expression. In neurodegenerative disorders improper RNA metabolism linked to THOC7 dysfunction can lead to neuronal degradation highlighting connections with proteins like TDP-43 which play a role in these conditions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human THOC7 protein (ab202166)
15% SDS-PAGE analysis of 3 μg ab202166.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com