JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB132365

Recombinant Human THRAP5 protein (GST tag N-Terminus)

Be the first to review this product! Submit a review

|

(1 Publication)

Recombinant Human THRAP5 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 860 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

DRIP92, THRAP5, MED16, Mediator of RNA polymerase II transcription subunit 16, Mediator complex subunit 16, Thyroid hormone receptor-associated protein 5, Thyroid hormone receptor-associated protein complex 95 kDa component, Vitamin D3 receptor-interacting protein complex 92 kDa component, Trap95

1 Images
SDS-PAGE - Recombinant Human THRAP5 protein (GST tag N-Terminus) (AB132365)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human THRAP5 protein (GST tag N-Terminus) (AB132365)

12.5% SDS-PAGE analysis of ab132365 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Accession

Q9Y2X0

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

style
left-column

Complementary Products

Primary Antibodies

AB130996

Anti-THRAP5 antibody

Immunoprecipitation - Anti-THRAP5 antibody (AB130996)

  • 5
  • 1
Proteins & Peptides

AB139631

Recombinant Human LCAT protein (denatured) (His tag N-Terminus)

SDS-PAGE - Recombinant Human LCAT protein (denatured) (His tag N-Terminus) (AB139631)

  • 0
  • 0
Proteins & Peptides

AB131700

Recombinant Human Dkk3 protein (denatured) (His tag N-Terminus)

SDS-PAGE - Recombinant Human Dkk3 protein (denatured) (His tag N-Terminus) (AB131700)

  • 0
  • 0
Assay Kits

AB267631

Human HGFA ELISA Kit

Sandwich ELISA - Human HGFA ELISA Kit (AB267631)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MCDLRRPAAGGMMDLAYVCEWEKWSKSTHCPSVPLACAWSCRNLIAFTMDLRSDDQDLTRMIHILDTEHPWDLHSIPSEHHEAITCLEWDQSGSRLLSADADGQIKCWSMADHLANSWESSVGSLVEGDPIVALSWLHNGVKLALHVEKSGASSFGEKFSRVKFSPSLTLFGGKPMEGWIAVTVSGLVTVSLLKPSGQVLTSTESLCRLRGRVALADIAFTGGGNIVVATADGSSASPVQFYKVCVSVVSEKCRIDTEILPSLFMRCTTDLNRKDKFPAITHLKFLARDMSEQVLLCASSQTSSIVECWSLRKEGLPVNNIFQQISPVVGDKQPTILKWRILSATNDLDRVSAVALPKLPISLTNTDLKVASDTQFYPGLGLALAFHDGSVHIVHRLSLQTMAVFYSSAAPRPVDEPAMKRPRTAGPAVHLKAMQLSWTSLALVGIDSHGKLSVLRLSPSMGHPLEVGLALRHLLFLLEYCMVTGYDWWDILLHVQPSMVQSLVEKLHEEYTRQTAALQQVLSTRILAMKASLCKLSPCTVTRVCDYHTKLFLIAISSTLKSLLRPHFLNTPDKSPGDRLTEICTKITDVDIDKVMINLKTEEFVLDMNTLQALQQLLQWVGDFVLYLLASLPNQGSLLRPGHSFLRDGTSLGMLRELMVVIRIWGLLKPSCLPVYTATSDTQDSMSLLFRLLTKLWICFPSTGPCSVWVLLGWQPLPGRDEGPASEPDEALVDECCLLPSQLLIPSLDWLPASDGLVSRLQPKQPLRLQFGRAPTLPGSAATLQLDGLARAPGQPKIDHLRRLHLGACPTEECKACTRCGCVTMLKSPNRTTAVKQWEQRWIKNCLCGGLWWRVPLSYP","proteinLength":"Full Length","predictedMolecularWeight":"121.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":860,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9Y2X0","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

THRAP5 also known as TRIP13 and thyroid hormone receptor-associated protein 5 is a protein with a molecular mass of approximately 36 kDa. This protein usually operates in the nucleus as a part of transcriptional regulation processes. It is strongly expressed in various tissues including the liver kidney and brain indicating its widespread presence in the body. Mechanically THRAP5 interacts with nuclear hormone receptors and acts as a coactivator which means that it helps facilitate the transcription of specific genes.
Biological function summary

THRAP5 functions as part of a larger complex called the Mediator complex which is essential for the regulation of gene expression. This complex serves as a bridge linking transcriptional activators and the RNA polymerase II machinery thereby influencing the transcription of genes downstream. THRAP5 is involved in various regulatory roles that include assisting in the activation of nuclear receptors. Its involvement in the complex structure of Mediator indicates its role in controlling gene expression.

Pathways

THRAP5 involves itself in the regulation of transcriptional processes and may influence the ERK/MAPK signaling pathway. This pathway is important for cell growth proliferation and differentiation. The protein interacts with transcription factors and other proteins like ERK1/2 to modulate these pathways. Its role in facilitating communication between hormone receptors and transcriptional machinery positions it as an important regulatory hub in cellular signaling.

THRAP5 interacts with various proteins involved in cancer and metabolic disorders. Studies have linked its aberrant expression to certain cancers including breast cancer where it might play a role in tumor progression. Additionally metabolic conditions like obesity have shown connections to THRAP5 potentially through interactions with PPARs (peroxisome proliferator-activated receptors) which are important for metabolic regulation. Understanding the contribution of THRAP5 in these diseases could offer insights into novel therapeutic targets.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Sequence similarities

Belongs to the Mediator complex subunit 16 family.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
See full target information MED16
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 8: PubMed32046307

2020

A Novel Combined Scientific and Artistic Approach for the Advanced Characterization of Interactomes: The Akirin/Subolesin Model.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Artigas-Jerónimo,Juan J Pastor Comín,Margarita Villar,Marinela Contreras,Pilar Alberdi,Israel León Viera,Leandro Soto,Raúl Cordero,James J Valdés,Alejandro Cabezas-Cruz,Agustín Estrada-Peña,José de la Fuente
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com