Recombinant Human THRAP5 protein is a Human Full Length protein, in the 1 to 860 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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- Vaccines 8:2020A Novel Combined Scientific and Artistic Approach for the Advanced Characterization of Interactomes: The Akirin/Subolesin Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Artigas-Jerónimo et. al.PubMed 32046307
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M C D L R R P A A G G M M D L A Y V C E W E K W S K S T H C P S V P L A C A W S C R N L I A F T M D L R S D D Q D L T R M I H I L D T E H P W D L H S I P S E H H E A I T C L E W D Q S G S R L L S A D A D G Q I K C W S M A D H L A N S W E S S V G S L V E G D P I V A L S W L H N G V K L A L H V E K S G A S S F G E K F S R V K F S P S L T L F G G K P M E G W I A V T V S G L V T V S L L K P S G Q V L T S T E S L C R L R G R V A L A D I A F T G G G N I V V A T A D G S S A S P V Q F Y K V C V S V V S E K C R I D T E I L P S L F M R C T T D L N R K D K F P A I T H L K F L A R D M S E Q V L L C A S S Q T S S I V E C W S L R K E G L P V N N I F Q Q I S P V V G D K Q P T I L K W R I L S A T N D L D R V S A V A L P K L P I S L T N T D L K V A S D T Q F Y P G L G L A L A F H D G S V H I V H R L S L Q T M A V F Y S S A A P R P V D E P A M K R P R T A G P A V H L K A M Q L S W T S L A L V G I D S H G K L S V L R L S P S M G H P L E V G L A L R H L L F L L E Y C M V T G Y D W W D I L L H V Q P S M V Q S L V E K L H E E Y T R Q T A A L Q Q V L S T R I L A M K A S L C K L S P C T V T R V C D Y H T K L F L I A I S S T L K S L L R P H F L N T P D K S P G D R L T E I C T K I T D V D I D K V M I N L K T E E F V L D M N T L Q A L Q Q L L Q W V G D F V L Y L L A S L P N Q G S L L R P G H S F L R D G T S L G M L R E L M V V I R I W G L L K P S C L P V Y T A T S D T Q D S M S L L F R L L T K L W I C F P S T G P C S V W V L L G W Q P L P G R D E G P A S E P D E A L V D E C C L L P S Q L L I P S L D W L P A S D G L V S R L Q P K Q P L R L Q F G R A P T L P G S A A T L Q L D G L A R A P G Q P K I D H L R R L H L G A C P T E E C K A C T R C G C V T M L K S P N R T T A V K Q W E Q R W I K N C L C G G L W W R V P L S Y P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Alternative names
DRIP92, THRAP5, MED16, Mediator of RNA polymerase II transcription subunit 16, Mediator complex subunit 16, Thyroid hormone receptor-associated protein 5, Thyroid hormone receptor-associated protein complex 95 kDa component, Vitamin D3 receptor-interacting protein complex 92 kDa component, Trap95
Recommended products
Recombinant Human THRAP5 protein is a Human Full Length protein, in the 1 to 860 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q9Y2X0-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M C D L R R P A A G G M M D L A Y V C E W E K W S K S T H C P S V P L A C A W S C R N L I A F T M D L R S D D Q D L T R M I H I L D T E H P W D L H S I P S E H H E A I T C L E W D Q S G S R L L S A D A D G Q I K C W S M A D H L A N S W E S S V G S L V E G D P I V A L S W L H N G V K L A L H V E K S G A S S F G E K F S R V K F S P S L T L F G G K P M E G W I A V T V S G L V T V S L L K P S G Q V L T S T E S L C R L R G R V A L A D I A F T G G G N I V V A T A D G S S A S P V Q F Y K V C V S V V S E K C R I D T E I L P S L F M R C T T D L N R K D K F P A I T H L K F L A R D M S E Q V L L C A S S Q T S S I V E C W S L R K E G L P V N N I F Q Q I S P V V G D K Q P T I L K W R I L S A T N D L D R V S A V A L P K L P I S L T N T D L K V A S D T Q F Y P G L G L A L A F H D G S V H I V H R L S L Q T M A V F Y S S A A P R P V D E P A M K R P R T A G P A V H L K A M Q L S W T S L A L V G I D S H G K L S V L R L S P S M G H P L E V G L A L R H L L F L L E Y C M V T G Y D W W D I L L H V Q P S M V Q S L V E K L H E E Y T R Q T A A L Q Q V L S T R I L A M K A S L C K L S P C T V T R V C D Y H T K L F L I A I S S T L K S L L R P H F L N T P D K S P G D R L T E I C T K I T D V D I D K V M I N L K T E E F V L D M N T L Q A L Q Q L L Q W V G D F V L Y L L A S L P N Q G S L L R P G H S F L R D G T S L G M L R E L M V V I R I W G L L K P S C L P V Y T A T S D T Q D S M S L L F R L L T K L W I C F P S T G P C S V W V L L G W Q P L P G R D E G P A S E P D E A L V D E C C L L P S Q L L I P S L D W L P A S D G L V S R L Q P K Q P L R L Q F G R A P T L P G S A A T L Q L D G L A R A P G Q P K I D H L R R L H L G A C P T E E C K A C T R C G C V T M L K S P N R T T A V K Q W E Q R W I K N C L C G G L W W R V P L S Y P
- Accession
- Q9Y2X0
- Protein length
- Full Length
- Predicted molecular weight
- 121.4 kDa
- Amino acids
- 1 to 860
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
- Sequence similarities
Belongs to the Mediator complex subunit 16 family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
THRAP5 also known as TRIP13 and thyroid hormone receptor-associated protein 5 is a protein with a molecular mass of approximately 36 kDa. This protein usually operates in the nucleus as a part of transcriptional regulation processes. It is strongly expressed in various tissues including the liver kidney and brain indicating its widespread presence in the body. Mechanically THRAP5 interacts with nuclear hormone receptors and acts as a coactivator which means that it helps facilitate the transcription of specific genes.
- Biological function summary
THRAP5 functions as part of a larger complex called the Mediator complex which is essential for the regulation of gene expression. This complex serves as a bridge linking transcriptional activators and the RNA polymerase II machinery thereby influencing the transcription of genes downstream. THRAP5 is involved in various regulatory roles that include assisting in the activation of nuclear receptors. Its involvement in the complex structure of Mediator indicates its role in controlling gene expression.
- Pathways
THRAP5 involves itself in the regulation of transcriptional processes and may influence the ERK/MAPK signaling pathway. This pathway is important for cell growth proliferation and differentiation. The protein interacts with transcription factors and other proteins like ERK1/2 to modulate these pathways. Its role in facilitating communication between hormone receptors and transcriptional machinery positions it as an important regulatory hub in cellular signaling.
- Associated diseases and disorders
THRAP5 interacts with various proteins involved in cancer and metabolic disorders. Studies have linked its aberrant expression to certain cancers including breast cancer where it might play a role in tumor progression. Additionally metabolic conditions like obesity have shown connections to THRAP5 potentially through interactions with PPARs (peroxisome proliferator-activated receptors) which are important for metabolic regulation. Understanding the contribution of THRAP5 in these diseases could offer insights into novel therapeutic targets.
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1 product image
SDS-PAGE - Recombinant Human THRAP5 protein (ab132365)
12.5% SDS-PAGE analysis of ab132365 stained with Coomassie Blue.
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