Recombinant Human Thrombomodulin Protein is a Human Fragment protein, in the 19 to 509 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, HPLC
- Biologically active
- No
Amino acid sequence
A P A E P Q P G G S Q C V E H D C F A L Y P G P A T F L N A S Q I C D G L R G H L M T V R S S V A A D V I S L L L N G D G G V G R R R L W I G L Q L P P G C G D P K R L G P L R G F Q W V T G D N N T S Y S R W A R L D L N G A P L C G P L C V A V S A A E A T V P S E P I W E E Q Q C E V K A D G F L C E F H F P A T C R P L A V E P G A A A A A V S I T Y G T P F A A R G A D F Q A L P V G S S A A V A P L G L Q L M C T A P P G A V Q G H W A R E A P G A W D C S V E N G G C E H A C N A I P G A P R C Q C P A G A A L Q A D G R S C T A S A T Q S C N D L C E H F C V P N P D Q P G S Y S C M C E T G Y R L A A D Q H R C E D V D D C I L E P S P C P Q R C V N T Q G G F E C H C Y P N Y D L V D G E C V E P V D P C F R A N C E Y Q C Q P L N Q T S Y L C V C A E G F A P I P H E P H R C Q M F C N Q T A C P A D C D P N T Q A S C E C P E G Y I L D D G F I C T D I D E C E N G G F C S G V C H N L P G T F E C I C G P D S A L A R H I G T D C D S G K V D G G D S G S G E P P P S P T P G S T L T P P A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis (PubMed:10761923). Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway (PubMed:29323190, PubMed:33836597, PubMed:9395524). Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition (PubMed:26663133). Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation (By similarity). Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response (PubMed:15841214).
Alternative names
CD141, THRM, THBD, Thrombomodulin, TM, Fetomodulin
Recommended products
Recombinant Human Thrombomodulin Protein is a Human Fragment protein, in the 19 to 509 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, HPLC
- Biologically active
- No
- Accession
- P07204-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- A P A E P Q P G G S Q C V E H D C F A L Y P G P A T F L N A S Q I C D G L R G H L M T V R S S V A A D V I S L L L N G D G G V G R R R L W I G L Q L P P G C G D P K R L G P L R G F Q W V T G D N N T S Y S R W A R L D L N G A P L C G P L C V A V S A A E A T V P S E P I W E E Q Q C E V K A D G F L C E F H F P A T C R P L A V E P G A A A A A V S I T Y G T P F A A R G A D F Q A L P V G S S A A V A P L G L Q L M C T A P P G A V Q G H W A R E A P G A W D C S V E N G G C E H A C N A I P G A P R C Q C P A G A A L Q A D G R S C T A S A T Q S C N D L C E H F C V P N P D Q P G S Y S C M C E T G Y R L A A D Q H R C E D V D D C I L E P S P C P Q R C V N T Q G G F E C H C Y P N Y D L V D G E C V E P V D P C F R A N C E Y Q C Q P L N Q T S Y L C V C A E G F A P I P H E P H R C Q M F C N Q T A C P A D C D P N T Q A S C E C P E G Y I L D D G F I C T D I D E C E N G G F C S G V C H N L P G T F E C I C G P D S A L A R H I G T D C D S G K V D G G D S G S G E P P P S P T P G S T L T P P A
- Accession
- P07204
- Protein length
- Fragment
- Predicted molecular weight
- 51.55 kDa
- Actual molecular weight
- 51.55 kDa
- Amino acids
- 19 to 509
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis (PubMed:10761923). Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway (PubMed:29323190, PubMed:33836597, PubMed:9395524). Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition (PubMed:26663133). Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation (By similarity). Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response (PubMed:15841214).
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Thrombomodulin also known as CD141 is a glycoprotein with a mass of approximately 74 kDa. It is an integral membrane protein expressed on the surface of endothelial cells. Thrombomodulin acts mechanically by binding thrombin an enzyme involved in blood coagulation to modulate its activity. This binding substantially alters thrombin's substrate specificity turning it into an anticoagulant enzyme that activates protein C instead of a procoagulant enzyme.
- Biological function summary
Thrombomodulin regulates blood coagulation and maintains hemostatic balance. It forms a complex with thrombin on endothelial cells facilitating the conversion of protein C to activated protein C (APC). Activated protein C plays a pivotal role in controlling blood clot formation by proteolytically inactivating Factors Va and VIIIa. This function is important for preventing excessive clotting while ensuring proper wound healing processes.
- Pathways
Thrombomodulin works chiefly within the anticoagulation pathway. It interacts directly with the thrombin-protein C pathway to achieve the activation of protein C. Additionally it interfaces with other pathways sharing mutual components such as endothelial cell proliferation and inflammation modulation. Related proteins include thrombin and protein C along with protein S which serves as a cofactor for APC's anticoagulant actions.
- Associated diseases and disorders
Thrombomodulin dysregulation can lead to thrombosis and vascular inflammation. A deficiency or altered expression of thrombomodulin has been linked to thromboembolic disorders and atherosclerosis. In these conditions improper activation of protein C in the presence of decreased thrombomodulin function can exacerbate clot formation. Monitoring the levels of proteins like thrombin and protein C within these diseases can provide insights into endothelial dysfunction and potential therapeutic approaches.
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2 product images
SDS-PAGE - Recombinant Human Thrombomodulin Protein (ab307351)
SDS-PAGE analysis of ab307351
HPLC - Recombinant Human Thrombomodulin Protein (ab307351)
HPLC analysis of ab307351
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