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AB123210

Recombinant Human ThrRS protein

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Recombinant Human ThrRS protein is a Human Full Length protein, in the 1 to 723 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

TARS, TARS1, Threonyl-tRNA synthetase, Threonyl-tRNA synthetase 1, ThrRS

1 Images
SDS-PAGE - Recombinant Human ThrRS protein (AB123210)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ThrRS protein (AB123210)

15% SDS-PAGE analysis of ab123210 (3 µg) under reducing condition and visualized by coomassie blue stain.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P26639

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Product previously known as Recombinant Human TARS protein.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMFEEKASSPSGKMGGEEKPIGAGEEKQKEGGKKKNKEGSGDGGRAELNPWPEYIYTRLEMYNILKAEHDSILAEKAEKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVIAKVNNVVWDLDRPLEEDCTLELLKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLEALCKKIIKEKQAFERLEVKKETLLAMFKYNKFKCRILNEKVNTPTTTVYRCGPLIDLCRGPHVRHTGKIKALKIHKNSSTYWEGKADMETLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKIGRDQELYFFHELSPGSCFFLPKGAYIYNALIEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSENMFSFEVEKELFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEVWDQAEKQLENSLNEFGEKWELNSGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQQFHDAKFMADIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKISGTVNIRTRDNKVHGERTISETIERLQQLKEFRSKQAEEEF","proteinLength":"Full Length","predictedMolecularWeight":"85.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":723,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P26639","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ThrRS also known as threonyl-tRNA synthetase is an enzyme that attaches the amino acid threonine to its corresponding tRNA (tRNA^Thr) an important step in the translation process during protein synthesis. ThrRS's molecular mass is approximately 80 kDa. It is expressed in various tissues across many organisms showing high conservation from bacteria to humans. This enzyme ensures accurate pairing of threonine with its tRNA which is critical for translating genetic information into functional proteins.
Biological function summary

Threonyl-tRNA synthetase plays a role in cellular function by ensuring proteins are synthesized accurately and efficiently. ThrRS is a standalone enzyme and does not form part of an enzyme complex. Its correct functioning is essential for maintaining cellular homeostasis and ensuring the fidelity of the genetic code. Incorrect aminoacylation by ThrRS can lead to translational errors which may manifest in malfunctioning proteins.

Pathways

The activity of threonyl-tRNA synthetase is integral to the protein synthesis pathway which is part of the larger biological process of gene expression. This pathway involves collaboration with other aminoacyl-tRNA synthetases and overlaps with translation mechanisms. ThrRS is related to proteins such as serine-tRNA synthetase and alanine-tRNA synthetase as they also participate in aminoacylation facilitating accurate protein synthesis by adding the right amino acids to tRNA molecules.

Problems associated with threonyl-tRNA synthetase can lead to conditions like Charcot-Marie-Tooth disease a disorder affecting peripheral nerves and certain types of cancer. Mutations in the ThrRS gene can alter its enzymatic activity or stability potentially disrupting the translation process. Moreover issues with ThrRS can interact with defective proteins like glycyl-tRNA synthetase further impacting cellular function and contributing to disease pathologies.
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Specifications

Form

Liquid

Additional notes

ab123210 was purified using conventional chromatography.

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General info

Function

Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction : threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr) (PubMed : 25824639, PubMed : 31374204). Also edits incorrectly charged tRNA(Thr) via its editing domain, at the post-transfer stage (By similarity).

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Post-translational modifications

ISGylated.

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Product protocols

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Target data

Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction : threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr) (PubMed : 25824639, PubMed : 31374204). Also edits incorrectly charged tRNA(Thr) via its editing domain, at the post-transfer stage (By similarity).
See full target information TARS1
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