Recombinant Human Thymidine Kinase 2 protein is a Human Full Length protein, in the 34 to 265 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M V Q R R A W P P D K E Q E K E K K S V I C V E G N I A S G K T T C L E F F S N A T D V E V L T E P V S K W R N V R G H N P L G L M Y H D A S R W G L T L Q T Y V Q L T M L D R H T R P Q V S S V R L M E R S I H S A R Y I F V E N L Y R S G K M P E V D Y V V L S E W F D W I L R N M D V S V D L I V Y L R T N P E T C Y Q R L K K R C R E E E K V I P L E Y L E A I H H L H E E W L I K G S L F P M A A P V L V I E A D H H M E R M L E L F E Q N R D R I L T P E N R K H C P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Phosphorylates thymidine, deoxycytidine, and deoxyuridine in the mitochondrial matrix (PubMed:11687801, PubMed:9989599). In non-replicating cells, where cytosolic dNTP synthesis is down-regulated, mtDNA synthesis depends solely on TK2 and DGUOK (PubMed:9989599). Widely used as target of antiviral and chemotherapeutic agents (PubMed:9989599).
Alternative names
2'-deoxyuridine kinase TK2, Deoxycytidine kinase TK2, Mt-TK, TK2
Recommended products
Recombinant Human Thymidine Kinase 2 protein is a Human Full Length protein, in the 34 to 265 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O00142-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Preservative: 1.7% Imidazole
Constituents: 20% Glycerol (glycerin, glycerine), 2.92% Sodium chloride, 0.32% Tris HCl, 0.002% PMSF
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M V Q R R A W P P D K E Q E K E K K S V I C V E G N I A S G K T T C L E F F S N A T D V E V L T E P V S K W R N V R G H N P L G L M Y H D A S R W G L T L Q T Y V Q L T M L D R H T R P Q V S S V R L M E R S I H S A R Y I F V E N L Y R S G K M P E V D Y V V L S E W F D W I L R N M D V S V D L I V Y L R T N P E T C Y Q R L K K R C R E E E K V I P L E Y L E A I H H L H E E W L I K G S L F P M A A P V L V I E A D H H M E R M L E L F E Q N R D R I L T P E N R K H C P
- Accession
- O00142
- Protein length
- Full Length
- Predicted molecular weight
- 30.2 kDa
- Amino acids
- 34 to 265
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab130043 is purified using conventional chromatography techniques.
General info
- Function
Phosphorylates thymidine, deoxycytidine, and deoxyuridine in the mitochondrial matrix (PubMed:11687801, PubMed:9989599). In non-replicating cells, where cytosolic dNTP synthesis is down-regulated, mtDNA synthesis depends solely on TK2 and DGUOK (PubMed:9989599). Widely used as target of antiviral and chemotherapeutic agents (PubMed:9989599).
- Sequence similarities
Belongs to the DCK/DGK family.
- Subcellular localisation
- Mitochondrion
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Thymidine Kinase 2 (TK2) is an enzyme that catalyzes the phosphorylation of deoxythymidine to deoxythymidine monophosphate within mitochondria. Also known by its gene symbol TK2 this protein has a mass of approximately 25 kilodaltons. TK2 is mainly expressed in mitochondria of tissues with high energy demands such as muscle and neural tissue due to their reliance on mitochondrial DNA replication.
- Biological function summary
The enzyme plays an important role in the maintenance and replication of mitochondrial DNA. TK2 forms a part of the mitochondrial nucleoside salvage pathway supporting the synthesis of nucleotides essential for DNA repair and replication. By ensuring a steady supply of nucleotides TK2 maintains the genetic integrity of mitochondrial DNA a critical process for cellular energy production and overall mitochondrial function.
- Pathways
TK2 contributes to the pyrimidine salvage pathway connecting with enzymes such as deoxycytidine kinase. It processes thymidine and other nucleosides to generate triphosphate nucleotides needed for mitochondrial DNA synthesis. Another significant pathway involving TK2 includes its interaction with nucleotide kinase pathways that help balance the pool of nucleotides essential for efficient DNA polymerization.
- Associated diseases and disorders
TK2 mutations lead to mitochondrial DNA depletion syndrome causing myopathic symptoms. This deficiency disrupts energy metabolism and results in muscle weakness and neural dysfunction. TK2's interaction with other proteins such as twinkle helicase further influences mitochondrial DNA stability and impacts these symptoms. Understanding this connection helps develop therapeutic strategies targeting mitochondrial disorders.
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1 product image
SDS-PAGE - Recombinant Human Thymidine Kinase 2 protein (ab130043)
15% SDS-PAGE showing ab130043 (3 µg).
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