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AB130043

Recombinant Human Thymidine Kinase 2 protein (His tag N-Terminus)

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Recombinant Human Thymidine Kinase 2 protein (His tag N-Terminus) is a Human Full Length protein, in the 34 to 265 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

2'-deoxyuridine kinase TK2, Deoxycytidine kinase TK2, Mt-TK, TK2

1 Images
SDS-PAGE - Recombinant Human Thymidine Kinase 2 protein (His tag N-Terminus) (AB130043)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Thymidine Kinase 2 protein (His tag N-Terminus) (AB130043)

15% SDS-PAGE showing ab130043 (3 µg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O00142

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Preservative: 1.7% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 2.92% Sodium chloride, 0.32% Tris HCl, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMVQRRAWPPDKEQEKEKKSVICVEGNIASGKTTCLEFFSNATDVEVLTEPVSKWRNVRGHNPLGLMYHDASRWGLTLQTYVQLTMLDRHTRPQVSSVRLMERSIHSARYIFVENLYRSGKMPEVDYVVLSEWFDWILRNMDVSVDLIVYLRTNPETCYQRLKKRCREEEKVIPLEYLEAIHHLHEEWLIKGSLFPMAAPVLVIEADHHMERMLELFEQNRDRILTPENRKHCP","proteinLength":"Full Length","predictedMolecularWeight":"30.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":265,"aminoAcidStart":34,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O00142","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thymidine Kinase 2 (TK2) is an enzyme that catalyzes the phosphorylation of deoxythymidine to deoxythymidine monophosphate within mitochondria. Also known by its gene symbol TK2 this protein has a mass of approximately 25 kilodaltons. TK2 is mainly expressed in mitochondria of tissues with high energy demands such as muscle and neural tissue due to their reliance on mitochondrial DNA replication.
Biological function summary

The enzyme plays an important role in the maintenance and replication of mitochondrial DNA. TK2 forms a part of the mitochondrial nucleoside salvage pathway supporting the synthesis of nucleotides essential for DNA repair and replication. By ensuring a steady supply of nucleotides TK2 maintains the genetic integrity of mitochondrial DNA a critical process for cellular energy production and overall mitochondrial function.

Pathways

TK2 contributes to the pyrimidine salvage pathway connecting with enzymes such as deoxycytidine kinase. It processes thymidine and other nucleosides to generate triphosphate nucleotides needed for mitochondrial DNA synthesis. Another significant pathway involving TK2 includes its interaction with nucleotide kinase pathways that help balance the pool of nucleotides essential for efficient DNA polymerization.

TK2 mutations lead to mitochondrial DNA depletion syndrome causing myopathic symptoms. This deficiency disrupts energy metabolism and results in muscle weakness and neural dysfunction. TK2's interaction with other proteins such as twinkle helicase further influences mitochondrial DNA stability and impacts these symptoms. Understanding this connection helps develop therapeutic strategies targeting mitochondrial disorders.
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Specifications

Form

Liquid

Additional notes

ab130043 is purified using conventional chromatography techniques.

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General info

Function

Phosphorylates thymidine, deoxycytidine, and deoxyuridine in the mitochondrial matrix (PubMed : 11687801, PubMed : 9989599). In non-replicating cells, where cytosolic dNTP synthesis is down-regulated, mtDNA synthesis depends solely on TK2 and DGUOK (PubMed : 9989599). Widely used as target of antiviral and chemotherapeutic agents (PubMed : 9989599).

Sequence similarities

Belongs to the DCK/DGK family.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Phosphorylates thymidine, deoxycytidine, and deoxyuridine in the mitochondrial matrix (PubMed : 11687801, PubMed : 9989599). In non-replicating cells, where cytosolic dNTP synthesis is down-regulated, mtDNA synthesis depends solely on TK2 and DGUOK (PubMed : 9989599). Widely used as target of antiviral and chemotherapeutic agents (PubMed : 9989599).
See full target information TK2
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