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AB95378

Recombinant Human Thymidylate Synthase protein (His tag N-Terminus)

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(1 Publication)

Recombinant Human Thymidylate Synthase protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 313 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

TS, OK/SW-cl.29, TYMS, Thymidylate synthase, TSase

2 Images
Western blot - Recombinant Human Thymidylate Synthase protein (His tag N-Terminus) (AB95378)
  • WB

Unknown

Western blot - Recombinant Human Thymidylate Synthase protein (His tag N-Terminus) (AB95378)

All lanes:

Anti-Thymidylate Synthase antibody (<a href='/en-us/products/unavailable/thymidylate-synthase-antibody-ab58287'>ab58287</a>) at 1 µg/mL

Lane 1:

Western blot - Recombinant Human Thymidylate Synthase protein (His tag N-Terminus) (ab95378) at 0.1 µg

Lane 2:

Western blot - Recombinant Human Thymidylate Synthase protein (His tag N-Terminus) (ab95378) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

true

Exposure time: 10s

SDS-PAGE - Recombinant Human Thymidylate Synthase protein (His tag N-Terminus) (AB95378)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Thymidylate Synthase protein (His tag N-Terminus) (AB95378)

SDS-PAGE image generated using 3ug ab95378

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P04818

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.5844% Sodium chloride, 0.315% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab95378 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/thymidylate-synthase-antibody-ab58287'>ab58287</a>.</p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMPVAGSELPRRPLPPAAQERDAEPRPPHGELQYLGQIQHILRCGVRKDDRTGTGTLSVFGMQARYSLRDEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVKIWDANGSRDFLDSLGFSTREEGDLGPVYGFQWRHFGAEYRDMESDYSGQGVDQLQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIEGYNPHPTIKMEMAV","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":313,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P04818","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thymidylate Synthase (TS) also known as TYMS or TSase is an important enzyme involved in the de novo synthesis of thymidylate a nucleotide necessary for DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) using 510-methylenetetrahydrofolate as a cofactor. It has an approximate molecular weight of 36 kDa. The enzyme is expressed in the cytoplasm of proliferating cells predominantly in tissues with high cellular turnover like bone marrow and the intestinal lining.
Biological function summary

Thymidylate synthase plays a critical role in DNA synthesis and cell division. As a member of the folate cycle it indirectly influences the supply of thymidine for DNA synthesis affecting cell proliferation. The enzyme generally functions as a homodimer which is necessary for its activity. Increased expression of TS usually correlates with high rates of cell division making it an important factor in rapidly growing tissues and tumors.

Pathways

Several important biological networks involve thymidylate synthase especially the folate and pyrimidine metabolism pathways. These pathways are essential for the maintenance of nucleotide pools within the cell. TS is functionally related to proteins like dihydrofolate reductase (DHFR) which also play roles in folate metabolism and DNA synthesis. Collectively these proteins ensure the proper balance of nucleotide precursors for DNA replication and repair.

Thymidylate synthase is closely associated with cancer and certain autoimmunity conditions. Overexpression of TS is commonly observed in various cancers as tumor cells require increased nucleotide synthesis for rapid proliferation. This makes TS a target for several anticancer drugs like 5-fluorouracil which inhibits TS activity. Additionally aberrations in enzymes like dihydropyrimidine dehydrogenase (DPD) could affect TS function leading to chemotherapeutic agent sensitivity in cancer treatments.
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Specifications

Form

Liquid

Additional notes

purified by using conventional chromatography techniques.

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General info

Function

Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.

Sequence similarities

Belongs to the thymidylate synthase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
See full target information TYMS
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of pharmaceutical sciences : offi 138:105008 PubMed31302213

2019

Pemetrexed-conjugated hyaluronan for the treatment of malignant pleural mesothelioma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Amano,Seiichi Ohta,Kazuma L Sakura,Taichi Ito
View all publications
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