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AB187470

Recombinant Human TIAL1 protein (His tag N-Terminus)

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Recombinant Human TIAL1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 375 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

Nucleolysin TIAR, TIA-1-related protein, TIAL1

1 Images
SDS-PAGE - Recombinant Human TIAL1 protein (His tag N-Terminus) (AB187470)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TIAL1 protein (His tag N-Terminus) (AB187470)

15% SDS-PAGE analysis of ab187470 (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q01085

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.03% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMMEDDGQPRTLYVGNLSRDVTEVLILQLFSQIGPCKSCKMITEHTSNDPYCFVEFYEHRDAAAALAAMNGRKILGKEVKVNWATTPSSQKKDTSNHFHVFVGDLSPEITTEDIKSAFAPFGKISDARVVKDMATGKSKGYGFVSFYNKLDAENAIVHMGGQWLGGRQIRTNWATRKPPAPKSTQENNTKQLRFEDVVNQSSPKNCTVYCGGIASGLTDQLMRQTFSPFGQIMEIRVFPEKGYSFVRFSTHESAAHAIVSVNGTTIEGHVVKCYWGKESPDMTKNFQQVDYSQWGQWSQVYGNPQQYGQYMANGWQVPPYGVYGQPWNQQGFGVDQSPSAAWMGGFGAQPPQGQAPPPVIPPPNQAGYGMASYQTQ","proteinLength":"Full Length","predictedMolecularWeight":"44 kDa","actualMolecularWeight":null,"aminoAcidEnd":375,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q01085","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIAL1 also known as TIA-1 related protein is a 42 kDa protein that plays a mechanical role in RNA processing. It belongs to the RNA-binding protein family and contains three RNA recognition motifs and a glutamine-rich domain. TIAL1 is widely expressed in various tissues including the spleen thymus and brain. It influences the regulation of mRNA stability and translation specifically recognizing and binding to U-rich elements in the 3’-untranslated regions of target mRNAs.
Biological function summary

TIAL1 influences gene expression by associating with stress granules cytoplasmic aggregates formed in response to cellular stress. This association helps inhibit translation initiation modulating the cellular stress response. TIAL1 is part of a complex with other RNA-binding proteins that integrate signaling events and manage mRNA turnover and stress granule dynamics. Through this interaction it affects apoptosis and contributes to cell survival under stress conditions.

Pathways

Studies demonstrate that TIAL1 plays a role in the apoptotic signaling pathway and the stress response pathway. By interacting with proteins such as eIF2α kinase TIAL1 helps mediate the cellular response to environmental and oxidative stress. These pathways maintain cellular homeostasis and TIAL1 assists in regulating translation during stress ensuring proper cell function.

TIAL1 has relevance in neurodegenerative diseases like Alzheimer's and amyotrophic lateral sclerosis (ALS). The formation of stress granules influenced by TIAL1 is connected to the pathological misfolding of proteins implicated by related proteins such as TDP-43 and FUS. Faulty regulation of these processes may lead to the progression of neurodegenerative conditions highlighting TIAL1's significant role in their development.
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Specifications

Form

Liquid

Additional notes

Purified using conventional chromatography techniques.

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General info

Function

RNA-binding protein involved in alternative pre-RNA splicing and in cytoplasmic stress granules formation (PubMed : 10613902, PubMed : 1326761, PubMed : 17488725, PubMed : 8576255). Shows a preference for uridine-rich RNAs (PubMed : 8576255). Activates splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on its own pre-mRNA and on TIA1 mRNA (By similarity). Promotes the inclusion of TIA1 exon 5 to give rise to the long isoform (isoform a) of TIA1 (PubMed : 17488725). Acts downstream of the stress-induced phosphorylation of EIF2S1/EIF2A to promote the recruitment of untranslated mRNAs to cytoplasmic stress granules (SG) (PubMed : 10613902). Possesses nucleolytic activity against cytotoxic lymphocyte target cells (PubMed : 1326761). May be involved in apoptosis (PubMed : 1326761).

Post-translational modifications

Phosphorylated by MAPK14 following DNA damage, releasing TIAR from GADD45A mRNA.

Subcellular localisation

Nucleus

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Product protocols

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Target data

RNA-binding protein involved in alternative pre-RNA splicing and in cytoplasmic stress granules formation (PubMed : 10613902, PubMed : 1326761, PubMed : 17488725, PubMed : 8576255). Shows a preference for uridine-rich RNAs (PubMed : 8576255). Activates splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on its own pre-mRNA and on TIA1 mRNA (By similarity). Promotes the inclusion of TIA1 exon 5 to give rise to the long isoform (isoform a) of TIA1 (PubMed : 17488725). Acts downstream of the stress-induced phosphorylation of EIF2S1/EIF2A to promote the recruitment of untranslated mRNAs to cytoplasmic stress granules (SG) (PubMed : 10613902). Possesses nucleolytic activity against cytotoxic lymphocyte target cells (PubMed : 1326761). May be involved in apoptosis (PubMed : 1326761).
See full target information Nucleolysin TIAR
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