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AB123160

Recombinant Human TICAM2 protein (His tag N-Terminus)

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Recombinant Human TICAM2 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 235 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

TIRAP3, TIRP, TRAM, TICAM2, TIR domain-containing adapter molecule 2, TICAM-2, Putative NF-kappa-B-activating protein 502, TRIF-related adapter molecule, Toll-like receptor adaptor protein 3, Toll/interleukin-1 receptor domain-containing protein, MyD88-4

1 Images
SDS-PAGE - Recombinant Human TICAM2 protein (His tag N-Terminus) (AB123160)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TICAM2 protein (His tag N-Terminus) (AB123160)

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q86XR7

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMGIGKSKINSCPLSLSWGKRHSVDTSPGYHESDSKKSEDLSLCNVAEHSNTTEGPTGKQEGAQSVEEMFEEEAEEEVFLKFVILHAEDDTDEALRVQNLLQDDFGIKPGIIFAEMPCGRQHLQNLDDAVNGSAWTILLLTENFLRDTWCNFQFYTSLMNSVNRQHKYNSVIPMRPLNNPLPRERTPFALQTINALEEESRGFPTQVERIFQESVYKTQQTIWKETRNMVQRQFIA","proteinLength":"Full Length","predictedMolecularWeight":"29.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":235,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q86XR7","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TICAM2 also known as TRAM (TRIF-related adaptor molecule) plays an important role in the innate immune response by mediating signaling from Toll-like receptors (TLRs). It has a mass of approximately 27 kDa. TICAM2 is mainly expressed in immune cells such as macrophages and dendritic cells. It engages in complex interactions with TLRs specifically TLR4 to trigger downstream signaling cascades.
Biological function summary

The molecule participates in the modulation of immune responses. TICAM2 is a part of the MyD88-independent pathway interacting closely with TRIF (TIR-domain-containing adapter-inducing interferon-β) in signaling complexes. This interaction leads to the activation of transcription factors that stimulate the production of type I interferons and other cytokines critical for fighting viral infections.

Pathways

TICAM2 functions in the TLR signaling pathway bridging TLR4 and TRIF to regulate immune responses. It plays a significant role in the TRAM-TRIF pathway. This pathway is essential for activating NF-κB and IRF3 which lead to the expression of inflammatory genes. Proteins like TLR4 and TRIF are closely related to TICAM2 in these signaling pathways and contribute to immune regulation.

TICAM2 is associated with immune system-related conditions such as sepsis and autoimmune diseases. Its role in modulating inflammatory responses links it to these disorders. The interaction with TLR4 is significant as it implicates TICAM2 in the hyperactivation of inflammatory signaling pathways leading to excessive immune responses in sepsis. Similarly an overactive TICAM2-related pathway can contribute to autoimmune diseases by sustaining chronic inflammation.
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Specifications

Form

Liquid

Additional notes

ab123160 was purified using conventional chromatography techniques and is >85% pure by SDS PAGE.

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General info

Function

Functions as a sorting adapter in different signaling pathways to facilitate downstream signaling leading to type I interferon induction (PubMed : 16603631, PubMed : 16757566, PubMed : 25385819, PubMed : 25825441). In TLR4 signaling, physically bridges TLR4 and TICAM1 and functionally transmits signal to TICAM1 in early endosomes after endocytosis of TLR4. In TLR2 signaling, physically bridges TLR2 and MYD88 and is required for the TLR2-dependent movement of MYD88 to endosomes following ligand engagement (PubMed : 25385819). Involved in IL-18 signaling and is proposed to function as a sorting adapter for MYD88 in IL-18 signaling during adaptive immune response (PubMed : 22685567). Forms a complex with RAB11FIP2 that is recruited to the phagosomes to promote the activation of the actin-regulatory GTPases RAC1 and CDC42 and subsequent phagocytosis of Gram-negative bacteria (PubMed : 30883606).. Isoform 2. Proposed to inhibit LPS-TLR4 signaling at the late endosome by interaction with isoform 1 thereby disrupting the association of isoform 1 with TICAM1. May be involved in TLR4 degradation in late endosomes.

Post-translational modifications

Phosphorylated by PRKCE in response to LPS. Phosphorylation is essential for its function. It is depleted from the membrane upon phosphorylation. Tyrosine phosphorylation is inhibited by phosphatase PTPN4.. Isoform 1 is myristoylated. Required for membrane association which is critical for its ability to initiate efficient signaling.

Subcellular localisation

Early endosome membrane

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Product protocols

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Target data

Functions as a sorting adapter in different signaling pathways to facilitate downstream signaling leading to type I interferon induction (PubMed : 16603631, PubMed : 16757566, PubMed : 25385819, PubMed : 25825441). In TLR4 signaling, physically bridges TLR4 and TICAM1 and functionally transmits signal to TICAM1 in early endosomes after endocytosis of TLR4. In TLR2 signaling, physically bridges TLR2 and MYD88 and is required for the TLR2-dependent movement of MYD88 to endosomes following ligand engagement (PubMed : 25385819). Involved in IL-18 signaling and is proposed to function as a sorting adapter for MYD88 in IL-18 signaling during adaptive immune response (PubMed : 22685567). Forms a complex with RAB11FIP2 that is recruited to the phagosomes to promote the activation of the actin-regulatory GTPases RAC1 and CDC42 and subsequent phagocytosis of Gram-negative bacteria (PubMed : 30883606).. Isoform 2. Proposed to inhibit LPS-TLR4 signaling at the late endosome by interaction with isoform 1 thereby disrupting the association of isoform 1 with TICAM1. May be involved in TLR4 degradation in late endosomes.
See full target information TICAM2
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