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AB151666

Recombinant Human TIE1 protein

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Recombinant Human TIE1 protein is a Human Fragment protein, in the 22 to 760 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

TIE, TIE1, Tyrosine-protein kinase receptor Tie-1

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, HPLC

applications

Biologically active

No

Accession

P35590

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 5.5 Constituents: 0.88% Sodium chloride, 0.4% MES

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

  • 5
  • 1
Assay Kits

AB100649

Human TIE1 ELISA Kit

Sandwich ELISA - Human TIE1 ELISA Kit (AB100649)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Primary Antibodies

AB151704

Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (AB151704)

  • 0
  • 0
Assay Kits

AB279955

Phosphotyrosine TIE1 ELISA Kit

Sandwich ELISA - Phosphotyrosine TIE1 ELISA Kit (AB279955)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months
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Sequence info

[{"sequence":"AVDLTLLANLRLTDPQRFFLTCVSGEAGAGRGSDAWGPPLLLEKDDRIVRTPPGPPLRLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVIYVHNSPGAHLLPDKVTHTVNKGDTAVLSARVHKEKQTDVIWKSNGSYFYTLDWHEAQDGRFLLQLPNVQPPSSGIYSATYLEASPLGSAFFRLIVRGCGAGRWGPGCTKECPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGISGCRGLTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRIPQILNMASELEFNLETMPRINCAAAGNPFPVRGSIELRKPDGTVLLSTKAIVEPEKTTAEFEVPRLVLADSGFWECRVSTSGGQDSRRFKVNVKVPPVPLAAPRLLTKQSRQLVVSPLVSFSGDGPISTVRLHYRPQDSTMDWSTIVVDPSENVTLMNLRPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPLLQPWLEGWHVEGTDRLRVSWSLPLVPGPLVGDGFLLRLWDGTRGQERRENVSSPQARTALLTGLTPGTHYQLDVQLYHCTLLGPASPPAHVLLPPSGPPAPRHLHAQALSDSEIQLTWKHPEALPGPISKYVVEVQVAGGAGDPLWIDVDRPEETSTIIRGLNASTRYLFRMRASIQGLGDWSNTVEESTLGNGLQAEGPVQESRAAEEGLDQQVDHHHHHH","proteinLength":"Fragment","predictedMolecularWeight":"81 kDa","actualMolecularWeight":null,"aminoAcidEnd":760,"aminoAcidStart":22,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P35590","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
A few weeks
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIE1 also known as Tyrosine Kinase with Immunoglobulin-like and EGF-like Domains 1 plays a role as a receptor tyrosine kinase. It has a molecular mass of approximately 130 kDa. TIE1 is mainly expressed in vascular endothelial cells and is involved in the vascular system. It shares structural characteristics with other tyrosine kinases providing a framework for signaling and interaction. TIE1 activation involves phosphorylation leading to subsequent signaling cascades in endothelial cells.
Biological function summary

TIE1 associates with angiogenesis and blood vessel integrity. It forms a complex with other proteins notably TIE2 within the endothelial cells where it modulates endothelial cell survival and proliferation. Its role becomes particularly significant in dynamic environments where new blood vessel formation or remodeling occurs. TIE1 interacts with angiopoietins providing a network essential for maintaining vascular homeostasis and integrity.

Pathways

TIE1 interacts within angiopoietin-TIE signaling pathways. This pathway plays a critical role in vascular maturation and stability. TIE1 working in conjunction with TIE2 and the angiopoietins orchestrates the intricate balance between vessel stabilization and sprouting needed for healthy vascular function. These interactions link TIE1 with core processes in endothelial cell communication and vascular development.

TIE1 shows association with cancer and atherosclerosis. Its interaction with TIE2 and angiopoietin proteins contributes to pathological angiogenesis often observed in tumor growth and progression. In atherosclerosis TIE1 influences endothelial dysfunction and vascular inflammation. These diseases highlight TIE1's contribution to pathological processes highlighting the importance of its precise regulation in maintaining vascular health.
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Specifications

Form

Lyophilized

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General info

Function

Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.

Post-translational modifications

Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.

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Product protocols

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Target data

Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
See full target information TIE1
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Product promise

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