Recombinant Human TIFA protein (His tag N-Terminus)
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Recombinant Human TIFA protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 184 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
T2BP, TIFA, TRAF-interacting protein with FHA domain-containing protein A, Putative MAPK-activating protein PM14, Putative NF-kappa-B-activating protein 20, TRAF2-binding protein
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human TIFA protein (His tag N-Terminus) (AB123199)
15% SDS-PAGE showing ab123199 (3μg).
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TIFA acts as a mediator in various cellular responses by forming signaling complexes. It possesses a threonine-phosphorylation motif necessary for the formation of oligomeric structures which facilitates its interactions with other proteins. Through this TIFA participates in immune response and inflammation regulation contributing to cellular resilience against external stresses.
Pathways
TIFA is an important player in the NF-κB signaling and Toll-like receptor pathways. It regulates protein interactions within these pathways such as TRAF2 and TRAF6 that help propagate proinflammatory signals. The induction of TIFA in these pathways leads to the activation of downstream effectors which result in targeted immune responses.
Specifications
Form
Liquid
Additional notes
ab123199 is purified using conventional chromatography techniques.
General info
Function
Adapter molecule that plays a key role in the activation of pro-inflammatory NF-kappa-B signaling following detection of bacterial pathogen-associated molecular pattern metabolites (PAMPs) (PubMed : 12566447, PubMed : 15492226, PubMed : 26068852, PubMed : 28222186, PubMed : 28877472, PubMed : 30111836). Promotes activation of an innate immune response by inducing the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism (PubMed : 15492226, PubMed : 26068852). TIFA-dependent innate immune response is triggered by ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria : ADP-Heptose is recognized by ALPK1, which phosphorylates TIFA at Thr-9, leading to TIFA homooligomerization and subsequent activation of pro-inflammatory NF-kappa-B signaling (PubMed : 30111836).
Sequence similarities
Belongs to the TIFA family.
Post-translational modifications
Phosphorylated at Thr-9 following detection of ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose) by ALPK1 (PubMed:30111836). Phosphorylation at Thr-9 by ALPK1 leads to the formation of an intermolecular binding between the FHA domain and phosphorylated Thr-9, promoting TIFA oligomerization and TIFA-mediated NF-kappa-B activation (PubMed:22566686, PubMed:26389808, PubMed:30111836).
Target data
Complementary Products
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Product promise
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