Recombinant human TIGIT protein (Fc Chimera Active) is a Human Fragment protein, in the 22 to 141 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA, Flow Cyt.
>95% SDS-PAGE
< 1 EU/µg
HEK 293 cells
Fc tag C-Terminus
SDS-PAGE, FuncS, ELISA, Flow Cyt
Yes
M M T G T I E T T G N I S A E K G G S I I L Q C H L S S T T A Q V T Q V N W E Q Q D Q L L A I C N A D L G W H I S P S F K D R V A P G P G L G L T L Q S L T V N D T G E Y F C I Y H T Y P D G T Y T G R I F L E V L E S S V A E H G A R F Q I P
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells (PubMed:19011627). Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades (PubMed:23154388). In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway (PubMed:24817116). Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity (PubMed:32503945).
VSIG9, VSTM3, VSTM3, TIGIT, VSIG9, T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3
Recombinant human TIGIT protein (Fc Chimera Active) is a Human Fragment protein, in the 22 to 141 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA, Flow Cyt.
>95% SDS-PAGE
< 1 EU/µg
HEK 293 cells
Fc tag C-Terminus
SDS-PAGE, FuncS, ELISA, Flow Cyt
Yes
Measured by its binding ability in a functional ELISA. Immobilized Recombinant human Poliovirus Receptor/PVR protein (ab220561) at 5 μg/mL (100 μL/well) can bind ab223110 with a linear range of 0.5-8 ng/mL.
Measured by its binding ability in a BLI assay. Loaded ab223110 on Protein A Biosensor, can bind Recombinant human Poliovirus Receptor/PVR protein (ab155723) with an affinity constant of 0.92 μM as determined in BLI assay.
No
Human
Reconstitute at 400 µg/mL in water
pH: 7.4
Constituents: 90% PBS, 10% Trehalose
M M T G T I E T T G N I S A E K G G S I I L Q C H L S S T T A Q V T Q V N W E Q Q D Q L L A I C N A D L G W H I S P S F K D R V A P G P G L G L T L Q S L T V N D T G E Y F C I Y H T Y P D G T Y T G R I F L E V L E S S V A E H G A R F Q I P
Fragment
40.1 kDa
22 to 141
Recombinant
Fc tag C-Terminus
Lyophilized
Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells (PubMed:19011627). Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades (PubMed:23154388). In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway (PubMed:24817116). Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity (PubMed:32503945).
Blue Ice
-20°C
-20°C
Upon reconstitution add a carrier protein (0.1% BSA)
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
This product is stable after storage at:
This supplementary information is collated from multiple sources and compiled automatically.
TIGIT also known as T cell immunoreceptor with Ig and ITIM domains is an immunoglobulin superfamily receptor. It weighs approximately 26 kDa. TIGIT commonly expresses on T cells especially on regulatory T cells (Tregs) and effector memory T cells. It also presents on natural killer (NK) cells. Among its alternate forms some notable ones also include the anti-TIGIT and biotinylated TIGIT antibodies used in experimental research to monitor immune responses.
TIGIT acts as an inhibitory receptor modulating immune responses. It belongs to the CD28 family and binds to poliovirus receptor (PVR) and nectin-2 on antigen-presenting cells. This interaction inhibits T cell activation and proliferation and increases Treg cell activity maintaining immune homeostasis. TIGIT forms a complex with other inhibitory receptors like PD-1 2B4 and CD96 to further regulate immune functions.
The interaction of TIGIT with its ligands significantly affects the immune checkpoint pathway. This involvement regulates T cell and NK cell activity. TIGIT is related to CD226 (DNAM-1) which competes for binding to its ligands influencing cellular signaling pathways. Through these pathways TIGIT signals through the downstream SHP-1 and SHP-2 proteins affecting immune cell signaling and response.
TIGIT has implications in cancer and autoimmune diseases. Its inhibitory function can allow tumor progression by dampening effective anti-tumor immunity. In cancer TIGIT’s activity often pairs with PD-1 both contributing to immune checkpoint blockade resistance necessary for tumor evasion. In autoimmune diseases increased TIGIT expression could result in reduced immune cell attack against self tissues showing its potential to modulate treatment strategies.
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Immobilized Recombinant human Poliovirus Receptor/PVR protein (Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) ab220561) at 5 μg/mL (100 μL/well) can bind ab223110 with a linear range of 0.5-8 ng/mL.
ab223110 analyzed by reduced SDS-PAGE and stained overnight with Coomassie Blue. The protein migrates as 42-50 kDa under reducing conditions due to glycosylation.
FACS assay shows that recombinant Human TIGIT Protein, Mouse IgG2a Fc Tag (ab223110) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.03 μg/ml
Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 μg/mL to 0.0097 μg/mL) were added into Human TIGIT (ab223110): Biotinylated Human CD112, Fc Tag (ab246048) binding reactions. Background was subtracted from data points before curve fitting.
Loaded ab223110 on Protein A Biosensor, can bind Recombinant human Poliovirus Receptor/PVR protein (Recombinant human Poliovirus Receptor/PVR protein (Active) ab155723) with an affinity constant of 0.92 μM as determined in BLI assay.
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