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Recombinant human TIGIT protein (Fc Chimera Active) is a Human Fragment protein, in the 22 to 141 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA, Flow Cyt.

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Images

ELISA - Recombinant human TIGIT protein (Fc Chimera Active) (AB223110), expandable thumbnail
  • SDS-PAGE - Recombinant human TIGIT protein (Fc Chimera Active) (AB223110), expandable thumbnail
  • Functional Studies - Recombinant human TIGIT protein (Fc Chimera Active) (AB223110), expandable thumbnail
  • Functional Studies - Recombinant human TIGIT protein (Fc Chimera Active) (AB223110), expandable thumbnail
  • Functional Studies - Recombinant human TIGIT protein (Fc Chimera Active) (AB223110), expandable thumbnail

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

Fc tag C-Terminus

Applications

SDS-PAGE, FuncS, ELISA, Flow Cyt

Biologically active

Yes

Amino acid sequence

M M T G T I E T T G N I S A E K G G S I I L Q C H L S S T T A Q V T Q V N W E Q Q D Q L L A I C N A D L G W H I S P S F K D R V A P G P G L G L T L Q S L T V N D T G E Y F C I Y H T Y P D G T Y T G R I F L E V L E S S V A E H G A R F Q I P

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Application

Flow Cyt

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells (PubMed:19011627). Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades (PubMed:23154388). In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway (PubMed:24817116). Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity (PubMed:32503945).

Alternative names

Recommended products

Recombinant human TIGIT protein (Fc Chimera Active) is a Human Fragment protein, in the 22 to 141 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA, Flow Cyt.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, FuncS, ELISA, Flow Cyt

Biological activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant human Poliovirus Receptor/PVR protein (ab220561) at 5 μg/mL (100 μL/well) can bind ab223110 with a linear range of 0.5-8 ng/mL.

Measured by its binding ability in a BLI assay. Loaded ab223110 on Protein A Biosensor, can bind Recombinant human Poliovirus Receptor/PVR protein (ab155723) with an affinity constant of 0.92 μM as determined in BLI assay.

Accession
Q495A1-1
Animal free

No

Species

Human

Reconstitution

Reconstitute at 400 µg/mL in water

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 90% PBS, 10% Trehalose

Sequence info

Amino acid sequence

M M T G T I E T T G N I S A E K G G S I I L Q C H L S S T T A Q V T Q V N W E Q Q D Q L L A I C N A D L G W H I S P S F K D R V A P G P G L G L T L Q S L T V N D T G E Y F C I Y H T Y P D G T Y T G R I F L E V L E S S V A E H G A R F Q I P

Accession

Q495A1

Protein length

Fragment

Predicted molecular weight

40.1 kDa

Amino acids

22 to 141

Nature

Recombinant

Tags

Fc tag C-Terminus

Specifications

Form

Lyophilized

General info

Function

Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells (PubMed:19011627). Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades (PubMed:23154388). In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway (PubMed:24817116). Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity (PubMed:32503945).

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon reconstitution add a carrier protein (0.1% BSA)

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

This product is stable after storage at:

  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TIGIT also known as T cell immunoreceptor with Ig and ITIM domains is an immunoglobulin superfamily receptor. It weighs approximately 26 kDa. TIGIT commonly expresses on T cells especially on regulatory T cells (Tregs) and effector memory T cells. It also presents on natural killer (NK) cells. Among its alternate forms some notable ones also include the anti-TIGIT and biotinylated TIGIT antibodies used in experimental research to monitor immune responses.

Biological function summary

TIGIT acts as an inhibitory receptor modulating immune responses. It belongs to the CD28 family and binds to poliovirus receptor (PVR) and nectin-2 on antigen-presenting cells. This interaction inhibits T cell activation and proliferation and increases Treg cell activity maintaining immune homeostasis. TIGIT forms a complex with other inhibitory receptors like PD-1 2B4 and CD96 to further regulate immune functions.

Pathways

The interaction of TIGIT with its ligands significantly affects the immune checkpoint pathway. This involvement regulates T cell and NK cell activity. TIGIT is related to CD226 (DNAM-1) which competes for binding to its ligands influencing cellular signaling pathways. Through these pathways TIGIT signals through the downstream SHP-1 and SHP-2 proteins affecting immune cell signaling and response.

Associated diseases and disorders

TIGIT has implications in cancer and autoimmune diseases. Its inhibitory function can allow tumor progression by dampening effective anti-tumor immunity. In cancer TIGIT’s activity often pairs with PD-1 both contributing to immune checkpoint blockade resistance necessary for tumor evasion. In autoimmune diseases increased TIGIT expression could result in reduced immune cell attack against self tissues showing its potential to modulate treatment strategies.

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5 product images

  • ELISA - Recombinant human TIGIT protein (Fc Chimera Active) (ab223110), expandable thumbnail

    ELISA - Recombinant human TIGIT protein (Fc Chimera Active) (ab223110)

    Immobilized Recombinant human Poliovirus Receptor/PVR protein (Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) ab220561) at 5 μg/mL (100 μL/well) can bind ab223110 with a linear range of 0.5-8 ng/mL.

  • SDS-PAGE - Recombinant human TIGIT protein (Fc Chimera Active) (ab223110), expandable thumbnail

    SDS-PAGE - Recombinant human TIGIT protein (Fc Chimera Active) (ab223110)

    ab223110 analyzed by reduced SDS-PAGE and stained overnight with Coomassie Blue. The protein migrates as 42-50 kDa under reducing conditions due to glycosylation.

  • Functional Studies - Recombinant human TIGIT protein (Fc Chimera Active) (ab223110), expandable thumbnail

    Functional Studies - Recombinant human TIGIT protein (Fc Chimera Active) (ab223110)

    FACS assay shows that recombinant Human TIGIT Protein, Mouse IgG2a Fc Tag (ab223110) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.03 μg/ml

  • Functional Studies - Recombinant human TIGIT protein (Fc Chimera Active) (ab223110), expandable thumbnail

    Functional Studies - Recombinant human TIGIT protein (Fc Chimera Active) (ab223110)

    Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 μg/mL to 0.0097 μg/mL) were added into Human TIGIT (ab223110): Biotinylated Human CD112, Fc Tag (ab246048) binding reactions. Background was subtracted from data points before curve fitting.

  • Functional Studies - Recombinant human TIGIT protein (Fc Chimera Active) (ab223110), expandable thumbnail

    Functional Studies - Recombinant human TIGIT protein (Fc Chimera Active) (ab223110)

    Loaded ab223110 on Protein A Biosensor, can bind Recombinant human Poliovirus Receptor/PVR protein (Recombinant human Poliovirus Receptor/PVR protein (Active) ab155723) with an affinity constant of 0.92 μM as determined in BLI assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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