Recombinant Human TIM 1 protein (Tagged) is a Human Fragment protein, in the 21 to 293 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
S V K V G G E A G P S V T L P C H Y S G A V T S M C W N R G S C S L F T C Q N G I V W T N G T H V T Y R K D T R Y K L L G D L S R R D V S L T I E N T A V S D S G V Y C C R V E H R G W F N D M K I T V S L E I V P P K V T T T P I V T T V P T V T T V R T S T T V P T T T T V P M T T V P T T T V P T T M S I P T T T T V L T T M T V S T T T S V P T T T S I P T T T S V P V T T T V S T F V P P M P L P R Q N H E P V A T S P S S P Q P A E T H P T T L Q G A I R R E P T S S P L Y S Y T T D G N D T V T E S S D G L W N N N Q T Q L F L E H S L L T A N T T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes This protein runs at a higher MW by SDS-PAGE due to glycosylation. |
Target data
Function
Phosphatidylserine receptor that plays an important functional role in regulatory B-cells homeostasis including generation, expansion and suppressor functions (By similarity). As P-selectin/SELPLG ligand, plays a specialized role in activated but not naive T-cell trafficking during inflammatory responses (PubMed:24703780). Controls thereby T-cell accumulation in the inflamed central nervous system (CNS) and the induction of autoimmune disease (PubMed:24703780). Regulates also expression of various anti-inflammatory cytokines and co-inhibitory ligands including IL10 (By similarity). Acts as a regulator of T-cell proliferation (By similarity). May play a role in kidney injury and repair (PubMed:17471468). (Microbial infection) Acts as a receptor for Hepatitis A virus. (Microbial infection) Acts as a receptor for Ebolavirus and Marburg virus by binding exposed phosphatidyl-serine at the surface of virion membrane (PubMed:21536871). Serves as a dual receptor for Ebolavirus by also interacting with envelope glycoprotein GP (PubMed:26487564). (Microbial infection) Acts as a receptor for Dengue virus by binding exposed phosphatidyl-serine at the surface of virion membrane (PubMed:23084921). TIM1 and Dengue virus are co-internalized during virus entry (PubMed:29742433). (Microbial infection) Acts as a receptor for Zika virus by binding to envelope protein E. (Microbial infection) Plays a positive role in Chikungunya virus cell entry.
Alternative names
CD365, KIM1, TIM1, TIMD1, HAVCR1, Hepatitis A virus cellular receptor 1, HAVcr-1, Kidney injury molecule 1, T-cell immunoglobulin and mucin domain-containing protein 1, T-cell immunoglobulin mucin receptor 1, T-cell membrane protein 1, KIM-1, TIMD-1, TIM, TIM-1
Recommended products
Recombinant Human TIM 1 protein (Tagged) is a Human Fragment protein, in the 21 to 293 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q96D42-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- S V K V G G E A G P S V T L P C H Y S G A V T S M C W N R G S C S L F T C Q N G I V W T N G T H V T Y R K D T R Y K L L G D L S R R D V S L T I E N T A V S D S G V Y C C R V E H R G W F N D M K I T V S L E I V P P K V T T T P I V T T V P T V T T V R T S T T V P T T T T V P M T T V P T T T V P T T M S I P T T T T V L T T M T V S T T T S V P T T T S I P T T T S V P V T T T V S T F V P P M P L P R Q N H E P V A T S P S S P Q P A E T H P T T L Q G A I R R E P T S S P L Y S Y T T D G N D T V T E S S D G L W N N N Q T Q L F L E H S L L T A N T T
- Accession
- Q96D42
- Protein length
- Fragment
- Amino acids
- 21 to 293
- Nature
- Recombinant
- Tags
- Avi tag C-Terminus, His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Phosphatidylserine receptor that plays an important functional role in regulatory B-cells homeostasis including generation, expansion and suppressor functions (By similarity). As P-selectin/SELPLG ligand, plays a specialized role in activated but not naive T-cell trafficking during inflammatory responses (PubMed:24703780). Controls thereby T-cell accumulation in the inflamed central nervous system (CNS) and the induction of autoimmune disease (PubMed:24703780). Regulates also expression of various anti-inflammatory cytokines and co-inhibitory ligands including IL10 (By similarity). Acts as a regulator of T-cell proliferation (By similarity). May play a role in kidney injury and repair (PubMed:17471468).
- Sequence similarities
Belongs to the immunoglobulin superfamily. TIM family.
- Post-translational modifications
Ubiquitinated at two lysine residues Lys-338 and Lys-346 on its cytoplasmic domain. Ubiquitination promotes receptor endocytosis and target receptors for lysosomal degradation and termination of receptor signaling.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TIM 1 also known as T-cell immunoglobulin and mucin domain 1 is a cell surface protein with a molecular mass of approximately 70 to 80 kDa. It is expressed predominantly in activated T cells and some epithelial cells. TIM 1 plays an important role in immune regulation and can be found at the surface of cells in various tissues including the liver and kidney. This protein can interact with other components which can influence immune response in the host.
- Biological function summary
The TIM 1 protein functions as a receptor involved in T cell activity modulation and innate immune response regulation. It is known to bind phosphatidylserine on apoptotic cells which fosters communication between dying cells and phagocytes. TIM 1 does not typically exist as part of a larger complex but its interactions with other molecules is important for its role in the immune system. The protein’s activity influences cytokine production and this can affect inflammation processes.
- Pathways
TIM 1 is involved in key immune response pathways like the signaling pathways of T cells and the broader adaptive immune response. This protein has been linked with the enhancement of Th2 cytokine production impacting allergic response signaling. It interacts with related proteins like TIM 3 and TIM 4 which together contribute to the fine-tuning of immune responses. Understanding TIM 1 within these pathways helps in grasping its regulatory potential in T-cell mediated immunity.
- Associated diseases and disorders
TIM 1 is implicated in conditions such as asthma and systemic lupus erythematosus (SLE). In asthma improperly regulated Th2 immune responses involve TIM 1 which can exacerbate allergic inflammations. This protein also plays a role in the pathogenesis of SLE a systemic autoimmune disease where TIM 1's regulation of immune tolerance and inflammation is critical. TIM 1’s involvement with other proteins like IL-4 and IL-13 in these disorders further highlights its significance in immune dysregulation processes.
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1 product image
SDS-PAGE - Recombinant Human TIM 1 protein (Tagged) (ab271762)
SDS-PAGE analysis of 3 μg ab271762.
This protein runs at a higher MW by SDS-PAGE due to glycosylation.
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