Recombinant Human TIM 4 protein (Tagged) is a Human Fragment protein, in the 25 to 315 aa range, expressed in HEK 293, with >=90% purity and suitable for WB, SDS-PAGE.
Images
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus His tag C-Terminus
- Applications
- WB, SDS-PAGE
- Biologically active
- No
Amino acid sequence
E T V V T E V L G H R V T L P C L Y S S W S H N S N S M C W G K D Q C P Y S G C K E A L I R T D G M R V T S R K S A K Y R L Q G T I P R G D V S L T I L N P S E S D S G V Y C C R I E V P G W F N D V K I N V R L N L Q R A S T T T H R T A T T T T R R T T T T S P T T T R Q M T T T P A A L P T T V V T T P D L T T G T P L Q M T T I A V F T T A N T C L S L T P S T L P E E A T G L L T P E P S K E G P I L T A E S E T V L P S D S W S S V E S T S A D T V L L T S K E S K V W D L P S T S H V S M W K T S D S V S S P Q P G A S D T A V P E Q N K T T K T G Q M D G I P M S M K N E M P I S Q L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes This protein runs at a higher MW by SDS-PAGE due to glycosylation. |
Target data
Function
Phosphatidylserine receptor that plays different role in immune response including phagocytosis of apoptotic cells and T-cell regulation. Controls T-cell activation in a bimodal fashion, decreasing the activation of naive T-cells by inducing cell cycle arrest, while increasing proliferation of activated T-cells by activating AKT1 and ERK1/2 phosphorylations and subsequent signaling pathways (By similarity). Also plays a role in efferocytosis which is the process by which apoptotic cells are removed by phagocytic cells (PubMed:32703939, PubMed:34067457). Mechanistically, promotes the engulfment of apoptotic cells or exogenous particles by securing them to phagocytes through direct binding to phosphatidylserine present on apoptotic cells, while other engulfment receptors such as MERTK efficiently recognize apoptotic cells and mediate their ingestion (PubMed:32640697). Additionally, promotes autophagy process by suppressing NLRP3 inflammasome activity via activation of LKB1/PRKAA1 pathway in a phosphatidylserine-dependent mechanism (By similarity). (Microbial infection) Plays a positive role in exosome-mediated trafficking of HIV-1 virus and its entry into immune cells.
Alternative names
TIM4, TIMD4, T-cell immunoglobulin and mucin domain-containing protein 4, TIMD-4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4, TIM-4
Recommended products
Recombinant Human TIM 4 protein (Tagged) is a Human Fragment protein, in the 25 to 315 aa range, expressed in HEK 293, with >=90% purity and suitable for WB, SDS-PAGE.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus His tag C-Terminus
- Applications
- WB, SDS-PAGE
- Biologically active
- No
- Accession
- Q96H15-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- E T V V T E V L G H R V T L P C L Y S S W S H N S N S M C W G K D Q C P Y S G C K E A L I R T D G M R V T S R K S A K Y R L Q G T I P R G D V S L T I L N P S E S D S G V Y C C R I E V P G W F N D V K I N V R L N L Q R A S T T T H R T A T T T T R R T T T T S P T T T R Q M T T T P A A L P T T V V T T P D L T T G T P L Q M T T I A V F T T A N T C L S L T P S T L P E E A T G L L T P E P S K E G P I L T A E S E T V L P S D S W S S V E S T S A D T V L L T S K E S K V W D L P S T S H V S M W K T S D S V S S P Q P G A S D T A V P E Q N K T T K T G Q M D G I P M S M K N E M P I S Q L
- Accession
- Q96H15
- Protein length
- Fragment
- Amino acids
- 25 to 315
- Nature
- Recombinant
- Tags
- Avi tag C-Terminus, His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Phosphatidylserine receptor that plays different role in immune response including phagocytosis of apoptotic cells and T-cell regulation. Controls T-cell activation in a bimodal fashion, decreasing the activation of naive T-cells by inducing cell cycle arrest, while increasing proliferation of activated T-cells by activating AKT1 and ERK1/2 phosphorylations and subsequent signaling pathways (By similarity). Also plays a role in efferocytosis which is the process by which apoptotic cells are removed by phagocytic cells (PubMed:32703939, PubMed:34067457). Mechanistically, promotes the engulfment of apoptotic cells or exogenous particles by securing them to phagocytes through direct binding to phosphatidylserine present on apoptotic cells, while other engulfment receptors such as MERTK efficiently recognize apoptotic cells and mediate their ingestion (PubMed:32640697). Additionally, promotes autophagy process by suppressing NLRP3 inflammasome activity via activation of LKB1/PRKAA1 pathway in a phosphatidylserine-dependent mechanism (By similarity).
- Sequence similarities
Belongs to the immunoglobulin superfamily. TIM family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TIM 4 also known as T cell immunoglobulin and mucin domain 4 is a protein of significant interest in immunology. It belongs to the TIM family of proteins which includes TIM 1 TIM 2 and TIM 3. TIM 4 weighs approximately 70 kDa and is mainly expressed on antigen-presenting cells including dendritic cells and macrophages. This protein plays an important role in immune response through its ability to recognize phosphatidylserine on apoptotic cells facilitating their clearance.
- Biological function summary
The function of TIM 4 extends to regulating immune homeostasis and tolerance. TIM 4 does not possess signaling motifs itself but often associates with other adaptor proteins to exert its biological effects. It acts independently or forms part of complexes that modulate cell signaling events influencing the outcome of immune responses. The protein plays important roles in maintaining the delicate balance of immune activation and suppression which is critical to preventing autoimmunity.
- Pathways
TIM 4 interaction occurs in pathways like the phosphatidylserine-mediated phagocytosis pathway which involves clearance of apoptotic cells. TIM 4 engages with phosphatidylserine a process important to maintaining tissue homeostasis and preventing inflammation. It works alongside other proteins in similar pathways including TIM 3 where together they mediate distinct and overlapping cellular processes involved in immune regulation.
- Associated diseases and disorders
TIM 4 presents significant implications in conditions including autoimmune diseases and cancer. Aberrant expression or function of TIM 4 associates with autoimmune disorders where it may contribute to the insufficient clearance of apoptotic cells subsequently promoting autoimmunity. In cancer altered TIM 4 activity can influence tumor progression by affecting immune evasion mechanisms. As TIM 4 interacts with TIM 3 these proteins together play roles in the development and progression of these diseases.
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2 product images
SDS-PAGE - Recombinant Human TIM 4 protein (Tagged) (ab271767)
SDS-PAGE analysis of 4 μg ab271767.
This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Western blot - Recombinant Human TIM 4 protein (Tagged) (ab271767)
All lanes: Western blot - Recombinant Human TIM 4 protein (Tagged) (ab271767) at 4 µg
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