Recombinant Human TIMM23/TIM23 protein is a Human Full Length protein, in the 1 to 209 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M E G G G G S G N K T T G G L A G F F G A G G A G Y S H A D L A G V P L T G M N P L S P Y L N V D P R Y L V Q D T D E F I L P T G A N K T R G R F E L A F F T I G G C C M T G A A F G A M N G L R L G L K E T Q N M A W S K P R N V Q I L N M V T R Q G A L W A N T L G S L A L L Y S A F G V I I E K T R G A E D D L N T V A A G T M T G M L Y K C T G G L R G I A R G G L T G L T L T S L Y A L Y N N W E H M K G S L L Q Q S L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane (PubMed:10339406). Has a role in the activation of stress-induced mitophagy by protecting PINK1 from OMA1-mediated degradation and facilitating its accumulation at the outer mitochondrial membrane in response to depolarization (PubMed:37160114).
Alternative names
TIM23, TIMM23, Mitochondrial import inner membrane translocase subunit Tim23
Recommended products
Recombinant Human TIMM23/TIM23 protein is a Human Full Length protein, in the 1 to 209 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- O14925-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M E G G G G S G N K T T G G L A G F F G A G G A G Y S H A D L A G V P L T G M N P L S P Y L N V D P R Y L V Q D T D E F I L P T G A N K T R G R F E L A F F T I G G C C M T G A A F G A M N G L R L G L K E T Q N M A W S K P R N V Q I L N M V T R Q G A L W A N T L G S L A L L Y S A F G V I I E K T R G A E D D L N T V A A G T M T G M L Y K C T G G L R G I A R G G L T G L T L T S L Y A L Y N N W E H M K G S L L Q Q S L
- Accession
- O14925
- Protein length
- Full Length
- Amino acids
- 1 to 209
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane (PubMed:10339406). Has a role in the activation of stress-induced mitophagy by protecting PINK1 from OMA1-mediated degradation and facilitating its accumulation at the outer mitochondrial membrane in response to depolarization (PubMed:37160114).
- Sequence similarities
Belongs to the Tim17/Tim22/Tim23 family.
- Subcellular localisation
- Mitochondrion inner membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as TIMM23.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TIMM23 also known as TIM23 protein is a critical component of the mitochondrial import machinery. It weighs approximately 24 kDa and is expressed in the inner mitochondrial membrane. TIMM23 is part of the translocase of inner mitochondrial membrane (TIM) complex which is essential for translocating proteins from the mitochondrial intermembrane space into the matrix. The TIM23 complex facilitates the passage of pre-sequence or matrix-targeted proteins that have signal sequences recognizable by the mitochondrial import machinery.
- Biological function summary
TIMM23 impacts mitochondrial function by cooperating with the TIM complex to ensure the proper import and processing of essential mitochondrial proteins. The complex known to include other subunits like TIMM17 and TIMM50 organizes the translocase activity required for maintaining mitochondrial homeostasis. TIMM23’s function is pivotal for energy production as it enables the importation of proteins important to several enzymatic processes particularly in oxidative phosphorylation.
- Pathways
Proteins like TIMM23 play a role in maintaining mitochondrial integrity which is essential for metabolic pathways. In particular TIMM23 is integral to the oxidative phosphorylation pathway linking it with proteins such as ATP synthase involved in ATP production. The electron transport chain also depends indirectly on TIMM23 for ensuring that necessary proteins reach the inner mitochondrial membrane implicating TIMM23 in cellular respiration processes.
- Associated diseases and disorders
TIMM23 aberrations can result in mitochondrial dysfunction contributing to conditions such as mitochondrial myopathy. The disruption in protein import can affect energy metabolism which is a common feature in diseases like Leigh syndrome. Other proteins such as complex I and complex IV of the electron transport chain can also exhibit dysfunction when affected by a compromised TIMM23 function highlighting its role in maintaining cellular energy efficiency.
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1 product image
SDS-PAGE - Recombinant Human TIMM23/TIM23 protein (ab153080)
ab153080 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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