Recombinant human TIMP1 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB, FuncS.
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- Human molecular genetics 26:1230-12462017Therapeutic potential of AAV-mediated MMP-3 secretion from corneal endothelium in treating glaucoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeffrey O'Callaghan,Darragh E Crosbie,Paul S Cassidy,Joseph M Sherwood,Cassandra Flügel-Koch,Elke Lütjen-Drecoll,Marian M Humphries,Ester Reina-Torres,Deborah Wallace,Anna-Sophia Kiang,Matthew Campbell,W Daniel Stamer,Darryl R Overby,Colm O'Brien,Lawrence C S Tam,Peter HumphriesPubMed 28158775
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
C T C V P P H P Q T A F C N S D L V I R A K F V G T P E V N Q T T L Y Q R Y E I K M T K M Y K G F Q A L G D A A D I R F V Y T P A M E S V C G Y F H R S H N R S E E F L I A G K L Q D G L L H I T T C S F V A P W N S L S L A Q R R G F T K T Y T V G C E E C T V F P C L S I P C K L Q S G T H C L W T D Q L L Q G S E K G F Q S R H L A C L P R E P G L C T W Q S L R S Q I A
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab82104 can be used as a WB positive control in conjunction with ab1827. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.
Alternative names
CLGI, TIMP, TIMP1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinases 1, EPA, Collagenase inhibitor, TIMP-1
Recommended products
Recombinant human TIMP1 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- Biological Activity : TIMP1 activity was measured by its ability to inhibit human MMP1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP1 concentration of approximately 0.5 μg/ml, when using an MMP1 concentration of 1.6 μg/ml.
- Accession
- P01033-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in water
- Concentration
Sequence info
Amino acid sequence
- C T C V P P H P Q T A F C N S D L V I R A K F V G T P E V N Q T T L Y Q R Y E I K M T K M Y K G F Q A L G D A A D I R F V Y T P A M E S V C G Y F H R S H N R S E E F L I A G K L Q D G L L H I T T C S F V A P W N S L S L A Q R R G F T K T Y T V G C E E C T V F P C L S I P C K L Q S G T H C L W T D Q L L Q G S E K G F Q S R H L A C L P R E P G L C T W Q S L R S Q I A
- Accession
- P01033
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purity is greater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per µg (1EU/µg).
General info
- Function
Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.
- Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
- Post-translational modifications
The activity of TIMP1 is dependent on the presence of disulfide bonds.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- A few weeks
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex (can be stored at 4C for up to 1 week).
For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tissue Inhibitor of Metalloproteinase 1 (TIMP1) is a protein with a molecular mass of approximately 28 kDa. The protein inhibits metalloproteinases by binding to them preventing the breakdown of the extracellular matrix. This action regulates extracellular environment and tissue remodeling. TIMP1 is also known by the name Erythroid-Potentiating Activity (EPA). It is expressed in various tissues including the liver and the brain and can be found in high levels in the blood serum.
- Biological function summary
TIMP1 plays several essential roles beyond inhibiting metalloproteinases. It supports cell proliferation and influences apoptosis. It is part of a larger TIMP family and does not function as part of a complex per se but interacts closely with matrix metalloproteinases (MMPs). By regulating MMP activity TIMP1 balances processes like tissue growth and inflammatory responses which are important for normal development and repair.
- Pathways
TIMP1 participates in the regulation of the matrix metalloproteinase pathway impacting tissue homeostasis and repair. It closely associates with proteins such as MMP-9 and MMP-2 within this pathway. TIMP1 also plays a role in cytokine signaling pathways which influence immune responses by interacting with other signaling molecules and receptors that govern these pathways.
- Associated diseases and disorders
TIMP1's regulation of MMPs links it to cancer and cardiovascular disease progression. Overexpression of TIMP1 associates with tumor growth and metastasis where it can modulate interactions with other proteins like MMP-9 leading to altered tissue invasion and angiogenesis. Additionally TIMP1 imbalance connects to tissue fibrosis in cardiovascular disorders working in tandem with proteins such as MMP-2 which affects the structural remodeling and function of tissues within the cardiovascular system.
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2 product images
Western blot - Recombinant human TIMP1 protein (ab82104)
All lanes: Anti-TIMP1 antibody [102D1] (ab1827) at 1 µg/mL
All lanes: Western blot - Recombinant human TIMP1 protein (ab82104) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 5000 µg/mL
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3min
Sandwich ELISA - Recombinant human TIMP1 protein (ab82104)
Standard curve for TIMP1 (Analyte: ab82104); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [2E7.1] to TIMP1 (ab28261) at 1μg/ml and Detector Antibody Rabbit polyclonal to TIMP1 - Carboxyterminal end (Anti-TIMP1 antibody - Carboxyterminal end ab38978) at 0.5μg/ml.
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