Recombinant Human TIMP1 protein (His tag) (Active) is a Human Full Length protein, in the 24 to 207 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
C T C V P P H P Q T A F C N S D L V I R A K F V G T P E V N Q T T L Y Q R Y E I K M T K M Y K G F Q A L G D A A D I R F V Y T P A M E S V C G Y F H R S H N R S E E F L I A G K L Q D G L L H I T T C S F V A P W N S L S L A Q R R G F T K T Y T V G C E E C T V F P C L S I P C K L Q S G T H C L W T D Q L L Q G S E K G F Q S R H L A C L P R E P G L C T W Q S L R S Q I A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.
Alternative names
CLGI, TIMP, TIMP1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinases 1, EPA, Collagenase inhibitor, TIMP-1
Recommended products
Recombinant Human TIMP1 protein (His tag) (Active) is a Human Full Length protein, in the 24 to 207 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- Yes
- Biological activity
- Fully active determined by its ability to inhibit human MMP2 cleavage of a chromogenic peptide substrate (Mca-PLGL-Dpa-AR-NH2). ED50 is ≤ 3.4 ng/ml, corresponding to specific activity of 2.9 x 105 units/mg.
- Accession
- P01033-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- C T C V P P H P Q T A F C N S D L V I R A K F V G T P E V N Q T T L Y Q R Y E I K M T K M Y K G F Q A L G D A A D I R F V Y T P A M E S V C G Y F H R S H N R S E E F L I A G K L Q D G L L H I T T C S F V A P W N S L S L A Q R R G F T K T Y T V G C E E C T V F P C L S I P C K L Q S G T H C L W T D Q L L Q G S E K G F Q S R H L A C L P R E P G L C T W Q S L R S Q I A
- Accession
- P01033
- Protein length
- Full Length
- Predicted molecular weight
- 20.71 kDa
- Amino acids
- 24 to 207
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
=95% by HPLC
General info
- Function
Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.
- Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
- Post-translational modifications
The activity of TIMP1 is dependent on the presence of disulfide bonds.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tissue Inhibitor of Metalloproteinase 1 (TIMP1) is a protein with a molecular mass of approximately 28 kDa. The protein inhibits metalloproteinases by binding to them preventing the breakdown of the extracellular matrix. This action regulates extracellular environment and tissue remodeling. TIMP1 is also known by the name Erythroid-Potentiating Activity (EPA). It is expressed in various tissues including the liver and the brain and can be found in high levels in the blood serum.
- Biological function summary
TIMP1 plays several essential roles beyond inhibiting metalloproteinases. It supports cell proliferation and influences apoptosis. It is part of a larger TIMP family and does not function as part of a complex per se but interacts closely with matrix metalloproteinases (MMPs). By regulating MMP activity TIMP1 balances processes like tissue growth and inflammatory responses which are important for normal development and repair.
- Pathways
TIMP1 participates in the regulation of the matrix metalloproteinase pathway impacting tissue homeostasis and repair. It closely associates with proteins such as MMP-9 and MMP-2 within this pathway. TIMP1 also plays a role in cytokine signaling pathways which influence immune responses by interacting with other signaling molecules and receptors that govern these pathways.
- Associated diseases and disorders
TIMP1's regulation of MMPs links it to cancer and cardiovascular disease progression. Overexpression of TIMP1 associates with tumor growth and metastasis where it can modulate interactions with other proteins like MMP-9 leading to altered tissue invasion and angiogenesis. Additionally TIMP1 imbalance connects to tissue fibrosis in cardiovascular disorders working in tandem with proteins such as MMP-2 which affects the structural remodeling and function of tissues within the cardiovascular system.
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2 product images
SDS-PAGE - Recombinant Human TIMP1 protein (His tag) (Active) (ab280386)
SDS-PAGE analysis of ab280386.
Biological Activity - Recombinant Human TIMP1 protein (His tag) (Active) (ab280386)
Fully active determined by its ability to inhibit human MMP2 cleavage of a chromogenic peptide substrate (Mca-PLGL-Dpa-AR-NH2). ED50 is ≤ 3.4 ng/ml, corresponding to specific activity of 2.9 x 10^5 units/mg.
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