Recombinant Human TIMP2 protein is a Human Full Length protein, in the 27 to 220 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
Images
Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, MS, HPLC
- Biologically active
No
Amino acid sequence
C S C S P V H P Q Q A F C N A D V V I R A K A V S E K E V D S G N D I Y G N P I K R I Q Y E I K Q I K M F K G P E K D I E F I Y T A P S S A V C G V S L D V G G K K E Y L I A G K A E G D G K M H I T L C D F I V P W D T L S T T Q K K S L N H R Y Q M G C E C K I T R C P M I P C Y I S S P D E C L W M D W V T E K N I N G H Q A K F F A C I K R S D G S C A W Y R G A A P P K Q E F L D I E D P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
Alternative names
Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2
Recommended products
Recombinant Human TIMP2 protein is a Human Full Length protein, in the 27 to 220 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
Alternative names
Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2
Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, MS, HPLC
- Biologically active
No
- Accession
- P16035-1
- Animal free
Yes
- Carrier free
Yes
- Species
Human
- Reconstitution
Reconstitute with phosphate buffered saline. Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
C S C S P V H P Q Q A F C N A D V V I R A K A V S E K E V D S G N D I Y G N P I K R I Q Y E I K Q I K M F K G P E K D I E F I Y T A P S S A V C G V S L D V G G K K E Y L I A G K A E G D G K M H I T L C D F I V P W D T L S T T Q K K S L N H R Y Q M G C E C K I T R C P M I P C Y I S S P D E C L W M D W V T E K N I N G H Q A K F F A C I K R S D G S C A W Y R G A A P P K Q E F L D I E D P
- Accession
- Protein length
Full Length
- Predicted molecular weight
21.81 kDa
- Actual molecular weight
21.81 kDa
- Amino acids
27 to 220
- Nature
Recombinant
Specifications
- Form
Lyophilized
- Additional notes
Purity by HPLC >=95%
General info
- Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
- Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
- Post-translational modifications
The activity of TIMP2 is dependent on the presence of disulfide bonds.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
Ambient
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TIMP2 also known as Tissue Inhibitor of Metalloproteinases 2 mechanistically inhibits the activity of matrix metalloproteinases (MMPs). It binds to MMPs regulating the extracellular matrix's remodeling. The protein has a molecular mass of around 21 kDa. TIMP2 is expressed in various tissues including the brain liver and kidneys where it maintains a balance between matrix degradation and production.
- Biological function summary
TIMP2 plays a significant role in tissue maintenance and repair by controlling extracellular matrix turnover. It forms complexes with MMPs such as MMP-2 inhibiting their proteolytic activities. The protein is also involved in cell proliferation and differentiation affecting processes like angiogenesis and wound healing. Its regulatory function aids in maintaining tissue homeostasis and preventing excessive matrix breakdown.
- Pathways
TIMP2 is integral to the matrix metalloproteinase pathway and the regulation of endothelial cell migration. The protein interacts closely with other MMPs influencing their activation and modulation. Through these pathways TIMP2 holds an important position in cellular migration and signaling ensuring precise matrix remodeling in response to physiological needs.
- Associated diseases and disorders
TIMP2 has significant links with cancer and fibrosis. Dysregulation of TIMP2 expression or its imbalance with MMPs can contribute to tumor progression and metastasis due to uncontrolled matrix degradation. Additionally abnormal TIMP2 levels associate with fibrotic diseases where excessive tissue deposition occurs. TIMP2's interaction with proteins like MMP-9 and MMP-2 highlights its involvement in these pathological conditions.
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3 product images
SDS-PAGE - Recombinant Human TIMP2 protein (ab287940)
SDS-PAGE analysis of ab287940
Mass Spectrometry - Recombinant Human TIMP2 protein (ab287940)
ESI-TOF analysis ab287940.
Predicted MW is 21812.05 Da (+/- 1-0 Da by ESI TOF). Observed MW is 21812.41 Da.
HPLC - Recombinant Human TIMP2 protein (ab287940)
HPLC analysis of ab287940.
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