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AB291057

Recombinant Human TIMP2 Protein (Active)

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Recombinant Human TIMP2 Protein (Active) is a Human Full Length protein, in the 27 to 220 aa range, with >95%, suitable for FuncS.

View Alternative Names

Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2

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Mass Spectrometry - Recombinant Human TIMP2 Protein (Active) (AB291057)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human TIMP2 Protein (Active) (AB291057)
Biological Activity - Recombinant Human TIMP2 Protein (Active) (AB291057)
  • Biological Activity

Supplier Data

Biological Activity - Recombinant Human TIMP2 Protein (Active) (AB291057)

Fully active determined by its ability to inhibit human MMP2 cleavage of a chromogenic peptide substrate (Mca-PLGLDpa-AR-NH2). ED50 is ≤ 4.9 ng/ml, corresponding to a specific activity of 2.0 x 105 units/mg.

SDS-PAGE - Recombinant Human TIMP2 Protein (Active) (AB291057)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TIMP2 Protein (Active) (AB291057)

SDS-PAGE analysis of ab291057

Key facts

Purity

>95% SDS-PAGE

Tags

Tag free

Applications

FuncS

applications

Biologically active

Yes

Biological activity

Fully active determined by its ability to inhibit human MMP2 cleavage of a chromogenic peptide substrate (Mca-PLGLDpa-AR-NH2).

ED50 is ≤ 4.9 ng/ml, corresponding to a specific activity of 2.0 x 105 units/mg.

Accession

P16035

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }
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Sequence info

[{"sequence":"CSCSPVHPQQAFCNADVVIRAKAVSEKEVDSGNDIYGNPIKRIQYEIKQIKMFKGPEKDIEFIYTAPSSAVCGVSLDVGGKKEYLIAGKAEGDGKMHITLCDFIVPWDTLSTTQKKSLNHRYQMGCECKITRCPMIPCYISSPDECLWMDWVTEKNINGHQAKFFACIKRSDGSCAWYRGAAPPKQEFLDIEDP","proteinLength":"Full Length","predictedMolecularWeight":"21.81 kDa","actualMolecularWeight":"21.81 kDa","aminoAcidEnd":220,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P16035","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIMP2 also known as Tissue Inhibitor of Metalloproteinases 2 mechanistically inhibits the activity of matrix metalloproteinases (MMPs). It binds to MMPs regulating the extracellular matrix's remodeling. The protein has a molecular mass of around 21 kDa. TIMP2 is expressed in various tissues including the brain liver and kidneys where it maintains a balance between matrix degradation and production.
Biological function summary

TIMP2 plays a significant role in tissue maintenance and repair by controlling extracellular matrix turnover. It forms complexes with MMPs such as MMP-2 inhibiting their proteolytic activities. The protein is also involved in cell proliferation and differentiation affecting processes like angiogenesis and wound healing. Its regulatory function aids in maintaining tissue homeostasis and preventing excessive matrix breakdown.

Pathways

TIMP2 is integral to the matrix metalloproteinase pathway and the regulation of endothelial cell migration. The protein interacts closely with other MMPs influencing their activation and modulation. Through these pathways TIMP2 holds an important position in cellular migration and signaling ensuring precise matrix remodeling in response to physiological needs.

TIMP2 has significant links with cancer and fibrosis. Dysregulation of TIMP2 expression or its imbalance with MMPs can contribute to tumor progression and metastasis due to uncontrolled matrix degradation. Additionally abnormal TIMP2 levels associate with fibrotic diseases where excessive tissue deposition occurs. TIMP2's interaction with proteins like MMP-9 and MMP-2 highlights its involvement in these pathological conditions.
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Specifications

Form

Lyophilized

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General info

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

Sequence similarities

Belongs to the protease inhibitor I35 (TIMP) family.

Post-translational modifications

The activity of TIMP2 is dependent on the presence of disulfide bonds.

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Product protocols

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Target data

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
See full target information TIMP2
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