Recombinant Human TIMP3 protein is a Human Full Length protein, in the 1 to 211 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M T P W L G L I V L L G S W S L G D W G A E A C T C S P S H P Q D A F C N S D I V I R A K V V G K K L V K E G P F G T L V Y T I K Q M K M Y R G F T K M P H V Q Y I H T E A S E S L C G L K L E V N K Y Q Y L L T G R V Y D G K M Y T G L C N F V E R W D Q L T L S Q R K G L N Y R Y H L G C N C K I K S C Y Y L P C F V T S K N E C L W T D M L S N F G Y P G Y Q S K H Y A C I R Q K G G Y C S W Y R G W A P P D K S I I N A T D P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
Alternative names
Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3
Recommended products
Recombinant Human TIMP3 protein is a Human Full Length protein, in the 1 to 211 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P35625-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M T P W L G L I V L L G S W S L G D W G A E A C T C S P S H P Q D A F C N S D I V I R A K V V G K K L V K E G P F G T L V Y T I K Q M K M Y R G F T K M P H V Q Y I H T E A S E S L C G L K L E V N K Y Q Y L L T G R V Y D G K M Y T G L C N F V E R W D Q L T L S Q R K G L N Y R Y H L G C N C K I K S C Y Y L P C F V T S K N E C L W T D M L S N F G Y P G Y Q S K H Y A C I R Q K G G Y C S W Y R G W A P P D K S I I N A T D P
- Accession
- P35625
- Protein length
- Full Length
- Amino acids
- 1 to 211
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
- Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tissue inhibitor of metalloproteinases 3 (TIMP3) is widely recognized for its role in inhibiting the activity of matrix metalloproteinases (MMPs). It has an approximate mass of 24 kDa and is often referred to by its alternate name MIG. TIMP3 is expressed in various tissues especially those involved in extracellular matrix composition such as the kidney and lungs. Its expression helps maintain the balance between matrix deposition and degradation which is critical for normal tissue homeostasis.
- Biological function summary
The function of TIMP3 extends beyond mere inhibition of MMPs; it is also involved in regulating angiogenesis and apoptosis. TIMP3 forms complexes with extracellular matrix components acting as an anchor that modulates cell-matrix interactions. This capacity to bind to matrix elements enhances its inhibitory activity against proteases making it an important regulator of extracellular matrix integrity.
- Pathways
TIMP3 plays significant roles in extracellular matrix remodeling and the inflammatory response. It participates in pathways involving structural organization and signaling mediation such as the NF-kB signaling cascade. TIMP3 regulates proteins like TNF-alpha and MMPs which are central to these pathways balancing inflammatory responses and tissue remodeling processes.
- Associated diseases and disorders
The imbalance of TIMP3 activity has been linked to chronic obstructive pulmonary disease (COPD) and age-related macular degeneration (AMD). In COPD impaired regulation of ECM turnover by TIMP3 leads to destructive lung tissue remodeling. In AMD the regulation of angiogenesis and degradation of ECM around the retina by TIMP3 is disturbed influencing disease progression. Its interaction with proteins such as VEGF is critical in these pathological contexts where altered TIMP3 expression or function can exacerbate disease states.
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1 product image
SDS-PAGE - Recombinant Human TIMP3 protein (ab159704)
ab159704 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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