Recombinant Human TIMP4 protein (His tag) is a Human Full Length protein, in the 30 to 224 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A D P C S C A P A H P Q Q H I C H S A L V I R A K I S S E K V V P A S A D P A D T E K M L R Y E I K Q I K M F K G F E K V K D V Q Y I Y T P F D S S L C G V K L E A N S Q K Q Y L L T G Q V L S D G K V F I H L C N Y I E P W E D L S L V Q R E S L N H H Y H L N C G C Q I T T C Y T V P C T I S A P N E C L W T D W L L E R K L Y G Y Q A Q H Y V C M K H V D G T C S W Y R G H L P L R K E F V D I V Q P H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
Alternative names
Metalloproteinase inhibitor 4, Tissue inhibitor of metalloproteinases 4, TIMP-4, TIMP4
Recommended products
Recombinant Human TIMP4 protein (His tag) is a Human Full Length protein, in the 30 to 224 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q99727-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A D P C S C A P A H P Q Q H I C H S A L V I R A K I S S E K V V P A S A D P A D T E K M L R Y E I K Q I K M F K G F E K V K D V Q Y I Y T P F D S S L C G V K L E A N S Q K Q Y L L T G Q V L S D G K V F I H L C N Y I E P W E D L S L V Q R E S L N H H Y H L N C G C Q I T T C Y T V P C T I S A P N E C L W T D W L L E R K L Y G Y Q A Q H Y V C M K H V D G T C S W Y R G H L P L R K E F V D I V Q P H H H H H H
- Accession
- Q99727
- Protein length
- Full Length
- Predicted molecular weight
- 23.5 kDa
- Amino acids
- 30 to 224
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
- Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tissue Inhibitor of Metalloproteinases 4 (TIMP4) a member of the TIMP family plays a mechanical role by inhibiting matrix metalloproteinases (MMPs). This inhibition helps regulate extracellular matrix (ECM) remodeling. TIMP4 weighs approximately 22 kDa and is expressed in various tissues including the heart brain and reproductive organs. It binds directly to MMPs forming stable complexes that prevent the degradation of ECM components.
- Biological function summary
TIMP4 contributes to maintaining tissue homeostasis and integrity. By regulating ECM degradation it influences processes such as cell migration proliferation and angiogenesis. TIMP4 does not work alone; it associates with enzymes like MMP-2 and MMP-14 modulating their activities to keep ECM dynamics in check. This regulation is essential for normal physiological functions and also during tissue repair.
- Pathways
TIMP4 participates in the balance between ECM synthesis and degradation. It takes part in the ECM receptor interaction and focal adhesion pathways. These pathways involve several proteins including integrins and collagens which interact with ECM components modulated by TIMP4 activity. TIMP4 helps coordinate cellular responses to environmental changes through these pathways thereby influencing cell behavior and tissue organization.
- Associated diseases and disorders
TIMP4 has associations with cardiovascular diseases and cancer. In cardiac contexts altered TIMP4 levels impact heart function and remodeling often linked to cardiovascular diseases. Its dysregulation can contribute to tumor progression in cancers by affecting ECM degradation and subsequent metastasis. TIMP4's interaction with proteins like MMP-9 links it to both tumor invasiveness and vascular remodeling in these disease states.
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1 product image
SDS-PAGE - Recombinant Human TIMP4 protein (His tag) (ab219421)
SDS-PAGE analysis of ab219421 (3μg) under reducing condition and visualized by coomassie blue stain.
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