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AB151809

Recombinant Human Tissue kallikrein protein

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Recombinant Human Tissue kallikrein protein is a Human Full Length protein, in the 19 to 262 aa range, expressed in HEK 293 cells, with >95%, < 0.1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

Kallikrein-1, Kidney/pancreas/salivary gland kallikrein, Tissue kallikrein, KLK1

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 0.1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

HPLC, SDS-PAGE

applications

Biologically active

No

Accession

P06870

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% Calcium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Protein previously labeled as Kallikrein 1.
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Sequence info

[{"sequence":"PPIQSRIVGGWECEQHSQPWQAALYHFSTFQCGGILVHRQWVLTAAHCISDNYQLWLGRHNLFDDENTAQFVHVSESFPHPGFNMSLLENHTRQADEDYSHDLMLLRLTEPADTITDAVKVVELPTQEPEVGSTCLASGWGSIEPENFSFPDDLQCVDLKILPNDECKKVHVQKVTDFMLCVGHLEGGKDTCVGDSGGPLMCDGVLQGVTSWGYVPCGTPNKPSVAVRVLSYVKWIEDTIAENSVDHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"28.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":262,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P06870","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue kallikrein also known as KLK1 is a serine protease enzyme that plays an important role in the cleavage of kininogen to produce kinins. This enzyme with a molecular weight of approximately 30 kDa exists in various tissues like the kidney pancreas and salivary glands. The protein is part of the kallikrein family and is expressed prominently in these areas. You can commonly measure its levels through ELISA assays labeled "kallikrein ELISA" or specific assays like the "kallikrein 3 ELISA."
Biological function summary

Tissue kallikrein contributes to vasodilation and blood pressure regulation by producing bradykinin a peptide with strong vasodilator properties. It does not function alone but may interact with other enzymes to amplify its effect. Tissue kallikrein belongs to a larger family of kallikrein-related peptidases which perform various physiological roles adding complexity to its functional network within the human body.

Pathways

This enzyme participates extensively in the kallikrein-kinin system. A significant interaction happens within the renin-angiotensin-aldosterone system contributing to blood pressure regulation fluid balance and cardiovascular function. It engages with related proteins such as kininogen and bradykinin establishing important checkpoints within these pathways.

Abnormalities in tissue kallikrein regulation link to hypertension and inflammatory diseases like rheumatoid arthritis. Dysregulation may alter interactions with proteins like kininogen leading to altered inflammatory responses or blood pressure issues. Understanding these relationships can inform therapeutic strategies targeting the kallikrein-kinin system for improved disease management.
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Specifications

Form

Liquid

Additional notes

Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.Supplied as a 0.2 µM filtered solution.

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General info

Function

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.. (Microbial infection) Cleaves Neisseria meningitidis NHBA in saliva; Neisseria is an obligate commensal of the nasopharyngeal mucosa.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Post-translational modifications

The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.

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Product protocols

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Target data

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.. (Microbial infection) Cleaves Neisseria meningitidis NHBA in saliva; Neisseria is an obligate commensal of the nasopharyngeal mucosa.
See full target information KLK1
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