Recombinant human Tissue Plasminogen Activator protein, Single Chain (Active)

Specifications

Form

Liquid

General info

Function

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (By similarity).

Sequence similarities

Belongs to the peptidase S1 family.

Post-translational modifications

The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.. Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.. N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.. Characterization of O-linked glycan was studied in Bowes melanoma cell line.